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201272201-208AsianJournalofEcotoxicologyVol．72012No．2201-2082011-11-282012-01-28No．2009ZX07317-0061985-E-mailmarilyn0402@163．com*CorrespondingauthorE-maildfhe@des．ecnu．edu．cn1112211*1．2000622．200062。JSRiverSXRiver、。、VibrioQinghaiensisQ67。。。3SXJS。SXJS5－8TDO。。1673-589720122-201-08X171．5AStudyonBiotoxicityofTypicalUrbanBlack-odorRiverWaterChenRuirui1CaiXiaoyan1YangBo12ShiHuahong2HuangMinsheng1HeDefu1*1．EnvironmentalScienceandTechnologyEastChinaNormalUniversityShanghai200062China2．StateKeyLaboratoryofEstuarineandCoastalResearchShanghai200062ChinaReceived28November2011accepted12January2012AbstractThepollutionofurbanblack-odorriversreceivesattentionrecently．Surveyandanalysisonbiologicaltoxicityofblack-odorriverwaterarenecessarytoidentifyandevaluatethewaterquality．Inpresentstudybiologi-caltoxicityofwaterintwotypicalblack-odorriversinWenzhoucityJiushanwaiRiverJSandShanxiaRiverSXtoBrachydaniorerioLuminescentbacteriatestandXenopustropicalisembryosweremonthlydetected．Resultsindicatedthatwaterfromthetworiverswastoxictothethreeorganisms．Toxicologicaleffectsofblack-odorriverwatermeasuredusingmortalityofzebrafishwasingoodagreementwithmeasuredusingtherelativeinhibitorylightrateofLuminescentbacteria．ComparativelyLuminescentbacteriaweremoresensitivetoblack-odorriverwaterthanzebrafish．InXenopustropicalisembryostesttoxicologicaleffectsofblack-odorwaterwerecharacterizedbyembryossurvivalrateandteratogenicrate．AllofthethreespeciestestsshowedthattoxicityofSXwaterwashigherthanthatofJSwaterwhichwasconsistentwiththeblack-odordegreeoftherivers．StatisticalanalysisresultsofLuminescentbacteriatoxicitytestshowedthatthecomprehensivetoxicityofSXandJSwashigherinsummerfromMaytoAugustthanthatinwinterandspring．Theseasonalchangesofwatertoxicityoftheblack-odorriverswas2027closelyrelatedtotemperatureanddissolvedoxygenvaluesDOofwater．Thepresentstudycanprovidetheevi-denceforthebio-toxicologicaltestofcontaminativewatertoevaluateandtreatblack-odorriverwater．KeywordsbiotoxicityBrachydaniorerioVibrioQinghaiensisXenopustropicalisblack-odorriverswaterquality1-2。80%3-56。7。。“”、、、8。ⅣVV9-12。JSSX1800m25．6m2034m9．8m。。。、、13。14。、1516。、、。1Materialsandmethods1．1Brachydaniorerio2±1cm500。Q67VibrioQinghaiQ67。XenopustropicalisNF10～11。1．217105。2010112011101。4℃18。1。JS1、JS2、JS3、JS4、JS5、JS6、JS7、JS8、JS9JS10。SX1、SX2、SX3、SX4SX5。1Fig．1SamplingsitesinJiushanwaiRiverJSandShanxiaRiverSXinWenzhoucity1．31．3．17d5%220317。24h。150、180、200、250280mg·L－15K2Cr2O71319。48h20。pH、。1．3．24℃10min4℃5mL0．8%NaCl21。3mL50μL15min9901LUMINOMETER。40．8%NaCl。“I%=IT－IT/IT×100%”。20、40、80、100、120、160、200、250300mg·L－122。1．3．315HCG23。2HCG2036h1003NF10～1124。4。5mL1025℃24h。48h。2Results2．1JSSXK2Cr2O7K2Cr2O72。K2Cr2O725180mg·L－1K2Cr2O7180～200mg·L－1K2Cr2O7200mg·L－1K2Cr2O7。2180200mg·L－1K2Cr2O724．75%36．16%。2Fig．2LinearrelationbetweenmortalityofBrachydaniorerioandtheconcentrationofpotassiumdichromate2010112011101。1。2010112011569。20101120114180mg·L－1K2Cr2O7。2011695JS280%6。SXJS69。JS2。2．2JSSX。3y=23．096+0．255xR2=0．96903。JSSX。20471%Table1MortalityofBrachydaniorerioexposedtowatersamplesfromJSandSXrivers2010201111121245678910JS100000050000JS200015101001009510080100JS3000000050020JS40000500100010JS5005000005525JS600000000000JS7010100000015105JS8000250000050JS900000000000JS100000005006005SX1000400010010010010040SX200035590100951001000SX3100000010010010010020SX40000020100951001000SX5000000100100100100100000000050003±n=4Fig．3LinearrelationshipbetweenrelativeinhibitorylightrateofVibrioQinghaiensisQ67andtheconcentrationsofphenol2010112011104-A、B。2010102011440%20115－940%4A5－780%。10JS29JS2。4B2010102011320116－980%。22．34±6．59℃pH7．49±0．39DO2．36±1．75mg·L－1。21．54±7．02℃pH7．40±0．24DO1．72±1．48mg·L－1。4CDpH7－9DOSX。JSSXT、pHDO4-CDITDOSX4D。2．3JSSXJSSX。520101120113、59JSSX。JS105950%p＜0．0180%22054ABn=4CD±n=10±n=5pH、TDOFig．4EffectsofwaterfromJSandSXriversonLuminousbacteriaAandBshowtherelativeinhibitoryrateofLuminescentbacteriaexposedtowatersamplesfromtworiversrespectively．CandDshowrelativeinhibitorylightrateofLuminescentbacteriapHtemperatureTdissolvedoxygenDOoftworiversineverymonth．5A。5、91180%380%p＜0．015B。。35p＜0．01911JS1010%p＞0．055C。5D9SX1SX2p＜0．01。。、。6、、、、。3Discussion。、JSSX。。。K2Cr2O7K2Cr2O720675ACBD。＊＊p＜0．01＊＊＊p＜0．001。±。Fig．5EffectsofwatersamplesfromJSandSXriversondevelopmentofXenopustropicalisembryosAandCshowsurvivalrateandaberrationrateofXenopustropicalisembryosexposedtowatersamplesfromJSriver．BandDshowfatalityrateandaberrationrateofXenopustropicalisembryosexposedtoSXwatersamples.6Fig．6TypicalteratogeniceffectsofwatersamplesMay2011fromJSandSXriversonXenopustropicalisembryos2207。1。Q6726。。、27-28。。。。。201169590%80%。。JS280%。。。JSSX20113、59。、、293031。、、。JSSX、。。JSSX。3。SXJS。DO。1969—、20。1．J．200622918－22ChengJWuENCheYeta1．StudyonkeyindicatorsforjudgingblackandodorouswaterinareaofplainriversystemJ．ChinaWater＆Wastewater200622918－22inChinese2．J．200834z262－65JinPFZhangLYXiongYeta1．TheblackandodorouswatertrainingandecologyrestorationprojectofShanghaibeachJ．Water＆WastewaterEngineering200834z262－65inChinese3．J．200935413－17QianCPWangDQChenZIeta1．Progressofbioremed-iati