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A2/N-SBＲ*111211.1101682.110000－A2/N-SBＲ。A2/N-SBＲNO－2－NTP0.53mg/L1.14mg/LTP88.8%COD107.21mg/L76.81mg/LPHB150.88mg/LTPTP2.6TPNO－2－N94%96%。A2/N-SBＲPHB。DOI10.13205/j．hjgc．201608014SHOＲTCUTDENITＲIFYINGPHOSPHOＲUSＲEMOVALINA2/N-SBＲPＲOCESSLIWei1LIUJing1MENGHai-ting1ZHULei2FUJin-xiang11.MunicipalandEnvironmentalEngineeringShenyangJianzhuUniversityShenyang110168China2.ShenyangInstituteofEnvironmentalScienceShenyang110000ChinaAbstractAlab-scaleA2/N-SBＲprocesswasoperatedtoinvestigatetheperformanceofbiologicalnutrientsremovalanddenitrifyingphosphorusremovaltheoperationeffectoftypicalcycleandmetabolicmechanismofdenitrifyingphosphorusremovalbacteriafromthedomesticsewage．TheresultsindicatedthatsimultaneousanoxicphosphateuptakeandbiologicaldenitrificationwithgoodperformancecouldbeobtainedintheA2/N-SBＲprocessoperatedinlong-termstableoperation．EffluentconcentrationofNO－2－NandTPwere0.53mg/Land1.14mg/LinthetypicalcycleandtheTPremovalefficiencywas88%．TheconcentrationCODandglycogendecreasedby107.21mg/Land76.81mg/LPHBasinternalcarbonsourceincreasedby150.88mg/LtheTPanaerobicconcentrationwas2.6timesoftheinitialconcentration．TheremovalefficiencyofTPandNO－2－Nwere94%and96%．TheeffectofdenitrifyingphosphorusremovalofA2/N-SBＲprocesswassignificantandstable．TheelectrondonorPHBinshortcutdenitrifyingphosphorusremovalprocesswassynthesizedbyexternalcarbonsourceandglycogen．Keywordsdenitrifyingphosphorusremovalshortcutnitrificationmetabolicmechanismremovalefficiency*2014ZX07202-0112015010692015K7007XKHY-89。2015－12－130“”、、。1-2。A2/N-SBＲSBＲA2/N-SBＲ3-6SBＲ、。Kuba7-8/NO－3－NO2PAOsDPB。26EnvironmentalEngineeringNO－2－N、NO－3－NO29SDPB。“”PAOs9-10。－A2/N-SBＲA2N-SBＲ。11.1－A2/N-SBＲ1N-SBＲ1A2-SBＲ1、2。N-SBＲ5L—3h—0.5h—0.5h—2A2-SBＲ30L—2h—2.5h—0.5h—0.5h—。SBＲNO－2－NN-SBＲA2-SBＲ。1N-SBＲA2-SBＲN-SBＲNO－2A2-SBＲNO－22N-SBＲNO－2A2-SBＲN-SBＲA2-SBＲ。3200mg/L。1.2NH4ClKH2PO4CaCl2、MgSO4·7H2O、H2O、NaHCO31。CODTN－TP－MLSS1A2/N-SBＲFig．1FlowchartofA2N-SBＲsystem1—A2-SBＲ2—N-SBＲ3—4—5—6—7—8—9—10—A2-SBＲ11—A2-SBＲ12—13—A-SBＲ14—N-SBＲ。2A2/N-SBＲFig．2SchematicdiagramofA2/N-SBＲsystem1Table1WastewaterqualityofA2N-SBＲmg/LN-SBＲA2-SBＲCOD30.2～50.540.2COD141.0～158.7150.2NH+4－N30～8055.0NH+4－N1.0～3.02.0TN32～8156.5TN1.6～4.02.4TP2.5～5.13.8TP7.8～13.010.0pH7.0～7.57.25pH7.5～8.07.8DOWTWDOpHOＲPWTWlevel2PHBPHB11－。2N-SBＲ25～30℃ρDO0.5～1.2mg/LpH7.2～7.5SＲT8dA2-SBＲ36WaterPollutionControl24℃pH7.8SＲT24d。2.1A2/N-SBＲCODN-SBＲρCOD30.2～50.5mg/L40.2mg/LN-SBＲA2-SBＲρCOD141.7～158.7mg/L150.2mg/L3。CODA2-SBＲA2-SBＲPHB。N-SBＲA2-SBＲSDPBPHBNO－2－N。CODA2-SBＲ。A2/N-SBＲρCOD22.72mg/LCOD80.49%～86.39%84.08%A2/N-SBＲCOD。—×—。3A2/N-SBＲCODFig．3ConcentrationvariationofCODintheA2N-SBＲsystem2.2A2/N-SBＲTN4A2/N-SBＲρTN9.20～23.25mg/L16.23mg/LN-SBＲρTN77.5～80.3mg/LA2-SBＲρTN1.76～2.07mg/L。N-SBＲTNA2-SBＲPHB。TN3.64mg/LTN78.26%～87.91%83.87%。—■—。4A2/N-SBＲTNFig．4ConcentrationvariationofTNintheA2N-SBＲsystem2.3A2/N-SBＲTP5TPA2/N-SBＲρTP6.5～7.2mg/L6.7mg/LN-SBＲρTP2.3～3.2mg/LA2-SBＲ7.6～8.4mg/L。N-SBＲA2-SBＲA2-SBＲρTP0.82～1.54mg/L1.1mg/L76.31%～89.58%83.55%CODTNTP。TPN-SBＲCODA2-SBＲCODTP。—■—TP。5A2/N-SBＲTPFig．5ConcentrationvariationofTPintheA2/N-SBＲsystem2.4A2/N-SBＲ6A2/N-SBＲ46EnvironmentalEngineeringCOD。N-SBＲTPρTP2.59mg/L。60minCOD42.64mg/LCOD93.7%。60minCODρNH+4－N4.85mg/L95.8%ρNO－2－N74.4mg/LρNO－3－N4.05mg/L94.8%。a—N-SBＲb—A2-SBＲ。—■—NH+4－N—●—NO－3－N—▲—NO－2－N—△—TP—○—COD。6Fig．6VarationofCODnitrogenandphosphorusinatypicalcycleA2-SBＲ120minCODTP19.38mg/LN-SBＲ。A2-SBＲCOD117.3mg/LCOD83.5%CODDPAOPHBCOD。TP18.13mg/L10.53mg/LDPAO。A2-SBＲTPρTP1.14mg/L88.8%。0.53mg/L。1.61mg/L0.78mg/L12-14。2.5COD、TP、NO－2－NPHBρMLSS2800mg/L。7。COD137.5mg/L30.29mg/L23.15mg/gPHBTPTPTP2.6PHB32.19mg/g。PHBATP。CODPHB、TPNO－2－NNO－2－N94%96%。NO－2－NPHB15-16。2PHB150.88mg/LCOD107.21mg/LPHBCODMinoPHB76.81mg/L74.07mg/LPHB69%PHB31%56WaterPollutionControl—□—COD—○——■—TP—●—NO－2－N—▲—PHB。7/Fig．7Substratetransformationinanaerobic/anoxicreaction、。19.55mg/LNO－2－N162.13mg/LPHB16.45mg/L。2/Table2Variablequantityofsubstrateinanaerobic/anoxicreactionCOD/mg·L－1－107.21－11.22PHB/mg·g－132.19－34.59COD/mg·L－1150.88－162.13/mg·g－1－23.1524.65COD/mg·L－1－76.8181.79NH+4/mg·L－1－0.68－1.04NO－2/mg·L－1－19.55TP/mg·L－1－11.0316.45/mg·g－15.488.39COD/mg·L－17.811.94C5H7O2N12.4%。。ρMLVSS/ρMLSS85%ρMLVSS/ρMLSS63%～70%。PHB89。PHBPHBPHB。31N-SBＲ8PHBFig．8ThedyeingpictureofPHB9Fig．9Thedyeingpictureofpoly-phosphateNO－2－NA2-SBＲSDPBNO－2－NA2/N-SBＲ32dCOD、TNTP84.08%、83.87%83.55%。2N-SBＲ60min42.64mg/LCOD300min94.8%。A2-SBＲ10.4mg/LTP88.8%0.53mg/L。3NADH2ATP66EnvironmentalEngineering。469%PHB32.19mg/gPHBPHBNO－2－N17.05mg/L。1．J．2012356209-213.2Kerrn-JespersenJPHenzeM．BiologicalphosphorusuptakeunderanoxicandaerobicconditionsJ．WaterＲes1993274617-624.3．SBＲJ．20082961526-1532.4WangYYPengYZLiTWetal．Phosphorusremovalunderanoxicconditionsinacontinuous-flowA2NtwosludgeprocessJ．WatSciTechnol200450637-44.5．A/AJ．201535755-59.6．pHJ．．20134452144-2149.7KubaTSmoldersGvanLoosdrechtMCM．Biologicalphosphorusremovalfromwastewaterbyanaerobic-anoxicsequencingbatchreactorJ．WaterSciTechnol1993275241-252.8BarkerPSDoldPL．DenitrificationbehaviourinbiologicalexcessphosphorusremovalactivatedsludgesystemsJ．WaterＲes1996304769-780.9HuJYOngSLNgWJe