A2N双污泥反硝化除磷系统微生物的先间歇后连续培养及快速启动杨庆娟

A2N1,1＊,1,2,1(1.,　100022;2.AdvancedWastewaterManagementCentre,TheUniversityofQueensland,Australia):,(DPB)A2N.A2N.,SBRAO()AA(),32d.SBR,,30d,99%.,A2N,11d,0,10.26mgL,COD19.56mgL,COD、、91%、100%、77%100%,A2N,.:;A2N;;;:X703　:A　:0250-3301(2008)08-2249-05:2007-08-22;:2007-09-22:(50521140075);“”;(50628808):(1983～),,,,E-mail:[e-mail]＊,E-mail:[e-mail]nectingCultivationoftheOrganismsandFastStart-upofA2NTwo-SludgeSystemYANGQing-juan1,WANGShu-ying1,LIUYing1,YUANZhi-guo2,GEChong1(1.BeijingKeyLaboratoryofWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing100022,China;2.AdvancedWastewaterManagementCentre,TheUniversityofQueensland,Australia)Abstract:Usingdomesticsewage,thecultivationofdenitrifyingphosphorusremovingbacteria(DPB)andstart-upofA2Ntwo-sludgesystemwereinvestigatedinalaboratory.TheA2NsystemstartedupfastbyusingthemethodthatfirstbringingupDPBandnitrificationbiofilmseparatelythenconnectingthemintheA2Nsystem.TheenrichmentofDPBusedactivatedsludgefromthesedimentationtankofawastewatertreatmentplantthatworkingaboutphosphorusremoval.Inasequencingbatchreactor(SBR),thesludgemixturewasoperatedunderanaerobic-aerobic(AO)conditionfirstly.Then,theactivatedsludgewasconductedunderanaerobic-anoxic(AA)condition.Duringtheexperimentperiodof32d,DPBwassuccessfullyenriched.Atthesametime,utilizingtheactivatedsludgethatenrichedofnitrifyingbacteria,nitrificationbiofilmwasgrownupinanotherSBR.After30d,theaverageammoniaremovalefficiencysteadilystayedabove99%.Later,usingthenitrificationbiofilmandtheactivatedsludgethatenrichedofDPB,theA2Nsystemwasconnectedfordealingwithdomesticsewage.After11d,thetreatmenteffectofA2Nsystemattainedsteady.Thetotalnitrogenandphosphorusconcentrationofeffluentare0,thenitrateconcentrationis10.26mgL,andCODis19.56mgL.TheremovalefficiencyofCOD,ammonia,totalnitrogenandphosphorusare91%,100%,77%and100%,respectively.EachresultshowsthattheA2Nsystemisstartedupsuccessfully.Keywords:denitrifyingphosphorusremovingbacteria(DPB);A2Ntwo-sludgesystem;sequencingbatchreactor(SBR);cultivation;faststartup　　,.,,,[1,2].Kerrn-Jespersen[3],,.,,[4～10].A2N[11],:①“”,COD29820088　　　　　　ENVIRONMENTALSCIENCEVol.29,No.8Aug.,2008DOI:10.13227/j.hjkx.2008.08.025PHB,PHB,,;②“”,2:DPB,2,,[4,12～15].A2N,,“”,.A2N,,SBR,,A2N[16,17].A2N,.1　1.1　SBR,70cm,20cm,,,12L.,.A2N1.、、、、,6、5.5、13、10、27.5L.,,,“”.　　,1.:,;:1　A2NFig.1　SchematicdiagramofA2Nsystem1　Table1　CharacteristicsofinfluentwastewaterCODmg·L-1BOD5mg·L-1NH+4-Nmg·L-1TNmg·L-1PO3-4-Pmg·L-1pH～105～453100～35021.89～61.1122.69～62.333.75～11.96.8～7.5340～40028518950.8252.264.557.23601.2　[18]COD:;:;:;TN:-;TP:;:;MLSS:;:;DO、:WTWDO;pHORPWTWlevel2.1.3　2,,.2250　　　　　　29,.A2N.,,.25℃,5～6mgL,3.5gL,2,12h..[4],,2.,AO,,.12LSBR,,4h,3h,30min,3,、;,,.SBR4h,(KNO3),3h,30min,3,、.,A2N,.A2N::47.8Ld;MLSS3000～4000mgL;4～5mgL1～2mgL;,,30%,,30%;22～25℃.2　2.1　2.2,50.5%,20d,10d99%.30d,,2　Fig.2　NH+4-Ninfluentandeffluentconcentrationandremovalrateduringtheperiodofcultivation,.2.2　2.2.1　,.3.2d,10.37mgL2.77mgL.7.6mgL,.5d、8d,,,8.32mgL12.83mgL,12d17.45mgL,,[19].3　、、Fig.3　PO3-4-Pconcentrationandabsorptivityafterinfluent,anaerobicandaerobiccondition2.2.2　,,22518:A2N.,,,.4.3d,11.7mgL7.7mgL.4mgL,,,[4].7、11、15d,,7d5.85mgL15d26.08mgL,.20d,15d,20d,,,.4　、、Fig.4　PO3-4-Pconcentrationandabsorptivityafterinfluent,anaerobicandanoxiccondition2.3　A2N,A2N,.COD、、5.5,A2N,COD80%,90%;80%;,.,5　Fig.5　NH+4-NandPO3-4-Premovalrateduringtheperiodofstart-up,,.11d,95%,COD90%,81.52%.,,,.,30%,,.,.,.6COD、、、.,COD、、207.6、51.02、3.960.97mgL.,PHB,,CODCOD68.8%,24.35mgL.,100%,,,PHB.2252　　　　　　29.0,10.26mgL,COD19.56mgL,COD、、91%、100%、77%100%.6　Fig.6　MassconcentrationofPO3-4-P,COD,NOx,NH+4-Nduringacycle32d11dA2N,.,,A2N.A2N:,,,.,2,.“”,.,,DNPAOs.3　(1)SBR,(AO),(AA),32d;,1,.A2N,,11d,.(2)A2N,,COD、、91%、100%、77%100%,0,10.26mgL,COD19.56mgL,.:[1]　LeeDS,JeonCO,ParkJM.Biologicalnitrogenremovalwithenhancedphosphateuptakeinasequencingbatchreactorusingsinglesludgesystem[J].WatRes,2001,35(16):3968-3976.[2]　,,,.A2O[J].,2005,56(8):1565-1570.[3]　Kerrn-JespersenJP,HenzeM.Biologicalphosphorusuptakeunderanoxicandaerobiccondition[J].WatRes,1993,27(4):617-624.[4]　KubaT,VanLoosdrechtMCM,HeijnenJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WatRes,1996,30(7):1702-1710.[5]　,,,.[J].,2003,29(8):33-35.[6]　MerzoukiM,BernetN,DelgenesJP,etal.BiologicaldenitrifyingphosphorusremovalinSBR:effectofaddednitrateconcentrationandsludgeretentiontime[J].WatSciTech,2001,43(3):191-194.[7]　,,,.[J].,2006,23(3):5-8.[8]　BarkerPS,DoldPL.Denitrificationbehaviourinbiologicalexcessphosphorusremovalactivatedsludgesystem[J].WatRes,1996,30(4):769-780.[9]　,,,.、[J].,2003,19(1):33.[10]　BortoneG,MarsiliLibelliS,TilcheA,etal.AnoxicphosphateuptakeintheDEPHANOXprocess[J].WatSciTech,1999,40(4-5):177-185.[11]　,,,.[J].,2002,18(9):4-7.[12]　KubaT,SmoldersG,vanLoosdrechtMCM,etal.Biologicalphosphorusremovalfromwastewaterbyanaerobic-anoxicsequencingbatchreactor[J].WatSciTech,1993,27(5-6):241-252.[1