AbstractAnanaerobic/anoxicsequencingbatchreactor(A2SBR)isusedtoinvestigatetheconditionsforstartingupasingleactivatedsludgesystemanditscapabilityofdenitrifyingdephosphorizationisevaluated.TheresultsindicatethattheA2SBRprocesscouldbestartedupandperformsteadilywithin29days,inaseasonalmannerofanaerobic-sludgesedimentationandwaterdischarge-anoxic-sludgesedimentationandwaterdischarge.Afterthe29thday,theSBRturnstohavearoutineperformance.TheHRTtakes3hoursinanaerobicphaseand4.5hoursinanoxicphase.WhentheinfluentCODinanaerobicphasekeepsat250mg·L-1andNO-3-Ninanoxicphaseofabout30mg·L-1,over88%ofCOD,90%ofNO-3-Nand95%ofPO3-4-PareremovedintheA2SBRprocesswithaneffluentconcentrationofCOD,NO-3-NandPO3-4-Pof28mg·L-1,3.35mg·L-1and0.57mg·L-1,respectively.Keywordsdenitrifyingdephosphorization;enitrifyingphosphorusremovingbacteria;A2SBR;start-up;capabilityofdenitrifyingdephosphorization0、,,“”“”,。,、。,A2/O,UCT,SBR,,[1-5]。①。,,,。②。,,A2SBR-SBR(A2SBR),,。,,COD250mg·L-1,NO-3-N30mg·L-1,“---”,29dA2SBR;“--”,A2SBR,HRT3h4.5h,90%95%,COD88%,COD,NO-3-NPO3-4-P28,3.35,0.57mg·L-1,。;;A2SBR;;X703A1000-7857(2008)22-0058-05,,,,150090Start-upandEvaluationofanAnaerobic/AnoxicSequencingBatchReactor(A2SBR)forDenitrifyingDephosphorization:2008-10-07:(50778052):,202,,E-mail:shiym@hit.edu.cnSHIYuming,MAFang,JIANGXinxin,WANGQiangStateKeyLaboratoryofUrbanWaterResourcesandEnvironment,SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China(Articles)582008,26(22),,,。,,,,,[2,6-10]。[1],,,。“”,β-(PHB),。CODO2,,[6]。,30%50%,,(VFAs)[3]。-,(DenitrifyingPhosphorusremovingBacteria,DPB),,。[4]。,DPB、,、;,DPBSBR(SequencingBatchReactor)。-SBR(Anaerobic/AnoxicSequencingBatchReactor,A2SBR),,DPB,。11.11。,SBR,25cm,50cm,19L;,pH(ORP),ORP。SBR,,。,。1.2SBR,。1.2.1,SBRCOD:(1~11d),COD150mg·L-1;(12~29d),COD250mg·L-1。SBR8h,(19L)3h,(,10.5L)30min,(19L)4h,(10.5L)30min;10.5L。COD,DPB[11],SBR,,,10.5L,,。1.2.2,SBR。,COD250mg·L-1,NO-3-N30mg·L-1;SBR8h,(18L)3h,KNO31L,(19L)4h20min,30min、(10.5L)10min;9.5L。3,5d,,A2SBR。(1~29d),SBR,(30d),,MLSS(,,MLSS,:mg/L)3.5g·L-1。pH,7.0±0.1。1.3,MLSS3.5g·L-1(19L)。1.4,A2SBR,。,,:NaAc(COD)1SBRFig.1SBRexperimentalapparatus(Articles)2008,26(22)59150~250mg·L-1,KH2PO4(PO3-4)19mg·L-1,MgSO4·7H2O0.08g·L-1,CaCl2·H2O0.05g·L-1,NH4Cl0.1g·L-1,1mL·L-1;(1L):0.9gFeCl3,0.15gH3BO3,0.03gCuSO4·5H2O,0.18gKI,0.06gMnCl2·4H2O,0.12gZnSO4·7H2O,0.15gCoCl2·7H2O,0.06gNa2MoO4·2H2O,10gEDTA。PO3-4-PNO-3-N,PO3-4-P19mg·L-1,NO-3-N,KNO3。A2SBR,,COD250mg·L-1;,SBR(18L)1LKNO3。1.5COD、NO-3-N、PO3-4-P、MLSSpH,[12]。,COD、PO3-4-PNO-3-N3。、PO3-4-PPO3-4-P,mg·L-1;A2SBR,mg·L-1。22.1A2SBRA2SBR,NO-3-N1~3d33mg·L-1,4750mg·L-1,25d;26~29d,NO-3-N56.6mg·L-1,23。SBRCOD150mg·L-1,。3d,4.63mg·L-1(2(a)),6.61mg·L-1(2(b)),A2SBR1.98mg·L-1(2(c));,NO-3-N68.5%(3),22.35mg·L-1NO-3-N。。,SBR,,8~11d,6.1mg·L-1(2(a));(2(b)),,6.68mg·L-175.2mg·L-1,,11,5.79mg·L-1。A2SBR3(2(c)),6,(-0.18mg·L-1)。NO-3-N1~7d33mg·L-150mg·L-1,2(a)、(b)A2SBR(c)Fig.2Phosphorusreleaseinanaerobicphase(a),absorptioninanoxicphase(b)netabsorptioninA2SBRprocess(c)duringthestart-upperiod3A2SBRNO3--NFig.3NO3--NremovalinanoxicphaseoftheA2SBRprocessduringthestart-upperiod(a)(b)(c)(Articles)602008,26(22)NO-3-N,63%,80.7%(3)。(),,[8]。12,SBR,COD250mg·L-1。,A2SBR,6d,18,2510.5mg·L-1(2(a));15,2513.58mg·L-1(2(b)),A2SBR3.1mg·L-1(2(c));NO-3-N3d,1482.3%1599%,23,99.4%(3)。(29),NO-3-N3.92mg·L-199.6%,A2SBR。,,DPB。,。,HRT(3h),(HAc),HAc,DPB,,DPB,,。,[4]。DPB,,HRT3h,(HAc),PHB;,PHB,。A2SBR,,HAc(150mg·L-1),DPBPHB,,,DPB。Keren-Jespersen[13],、PHB,HAc,PHB。,,(HAc),,。2.2A2SBR29d,A2SBRNO-3-N3.92mg·L-199.6%,。30,SBR“→→”,PO3-4-PCOD10.5mg·L-1250mg·L-1,NO-3-N30mg·L-1,MLSS3.5g·L-1。5d,。4A2SBR35~39d,PO3-4-P、CODNO-3-N。,,COD1h247mg·L-127mg·L-1,PO3-4-P10.5mg·L-122.9mg·L-1,(HAc)。(3h),CODPO3-4-P27mg·L-124.5mg·L-1。,,1h,NO-3-NPO3-4-P31.79mg·L-124.72mg·L-1,12.12mg·L-17.76mg·L-1,6mg·g-1MLSS·h-14.89mg·g-1MLSS·h-1,PO3-4-PNO-3-N1.22mgN·mg-1P。,,,4.5h,,NO-3-NPO3-4-P3.35mg·L-10.57mg·L-1,A2SBR90%95%,COD88%。,COD28mg·L-1,,,,DPB,PHB。5A2SBRNO-3-NPO3-4-P。,NO-3-NPO3-4-P,:y=0.8048x+0.482。,xNO-3-N,yPO3-4-P,R20.996。,NO-3-N4A2SBRFig.4PO43--PandNO3--NremovalintheA2SBRprocessundersteadystate(Articles)2008,26(22)61,。A2SBR,CODDPBPHB,,PHBDPB,“”,,COD/TN[5,14]。31),COD150mg·L-1,A2SBRDPB,。COD250mg·L-1,NO-3-N30mg·L-1,———,29dA2SBR,NO-3-N3.92mg·L-199.6%。2)A2SBR,COD250mg·L-1,NO-3-N30mg·L-1,HRT3h4.5h,90%95%,COD88%,COD,NO-3-NPO3-4-P28,3.350.57mg·L-1。3)A2SBR,NO-3-NPO3-4-P,CODDPBPHB,PHBDPB,“”,。(References)[1],.[J].,2008,34(3):7-10.WanJinbao,WangJianyong.TechnologyofWaterTreatment,2008,34(3):7-10.[2]TsunedaS,OhnoT,SoejimaK,etal.Simultaneousnitrogenandphosphorusremovalusingdenitrifyingphosphate-accumulatingorganismsinasequencingbatchreactor[J].BiochemicalEngineeringJournal,2006,27(3):191-196.[3].[J].,2007,8:5-7.ZhangBao.AppliedEnergyTechnology,2007,8:5-7.[4],,./SBR[J].,2003,4(6):9-12.LiYongzhi,PengYongzhen,WangShuying.TechniquesandEquipmentforEnvironmentalPollutionControl,2003,4(6):9-12.[5],,,.A2N[J].,2003,19(9):8-11.WangYayi,DuHong,PengYongzhen,etal.ChinaWaterandWastewater,2003,19(9):8-11.[6],,.[J].,2003,22(12):883-887.FuGang,HeQunbiao,ZhouZengyan.Shangha