AASBR中PHB转化与反硝化吸磷的关系研究丁彩娟

A/ASBRPHB*丁彩娟,　吉芳英,　高小平,　张良金,　张　斌(　,　400045):通过COD浓度对A/ASBR反硝化除磷脱氮系统的影响试验表明,过高或过低的COD都不利于反硝化除磷系统的正常运行,当COD=220～300mg/l时,可以获得较为理想的处理效果。发现了缺氧段残存的外碳源有机物和厌氧储存的胞内碳源PHB对反硝化除磷过程的影响;试验结果进一步表明以PHB为碳源的反硝化除磷过程中,PHB的消耗与反硝化除磷脱氮具有良好的相关关系,并且2mgNO3--N的转化可以促进1mgPO3-4-P的吸收。:生物除磷脱氮;A/ASBR;反硝化除磷;PHB:X703　　:A　　:1006-7329(2005)03-0080-05StudyoftheCorrelationbetweentheTransformationofPHBandDenitrifyingPhosphorusAbsorptioninA/ASBRDINGCai-juan,JIFang-ying,GAOXiao-ping,ZHANGLiang-jin,ZHANGBin(TheKeyLaboratoryofMinistryofEducationforEcologyEnvironmentinThreeGorgesAreas,ChongqingUniversity,Chongqing400045,P.R.China)Abstract:BythestudyoftheinfluenceofCODconcentrationondenitrifyingphosphorremovalofA/ASBR(anaerobic/aerobicsequencebioreactor),itwasshownthatthefavorabletreatmenteffecthadbeengainedwiththeCODconcentrationbetween220and300mg/l;thehigherorlowerconcentrationofCODwasunfavor-abletotheworkofthesystem.Inourtest,ithasbeenconfirmedthattherudimentalcarbonresourceorganismandcelldepositorPHBdepositedinanaerobicstagehasasignificantinfluenceontheprocessofdenitrifyingphosphorousremova.lThebettercorrelationhasbeenobservedalsobetweentheconsumptionofPHBandde-nitrifyingphosphorousremovalwhentheuseofcelldepositorsPHBascarbonresourceisconcerned.Itindi-catesthatthedeoxidizationof2mgnitratemayacceleratetheabsorptionof1mgphosphorous.Keywords:biologicalnutrientandphosphorousremoval;A/ASBR;denitrifyingphosphorousremoval;PHB[1,2,3],、,,(VFA):PHBPAOs;。,DPB,PHB[3],PHB,,,。,,Kerrn-Jes-persen(1994),、PHB,第27卷　第3期2005年6月　　　　　　　重庆建筑大学学报JournalofChongqingJianzhuUniversity　　　　　　　Vo.l27　No.3Jun.2005*:2005-02-27:(50278101);(1-57):(1977-),,,,。,A/ASBR,COD,PHB。1　1.1　A/ASBR,24cm、30cm,10L。,。SBR、。、,0.55～0.7,SBR8h,:2.5h、4h、1.5h,。1.2　。,A/O(/),1mg/l、90%,A/A(/),。1　A/ASBRCOD、NO-3-N,///,、CODA/ASBR,A/ASBR,NO-3-N=15～30mg/L。2,/。A/ASBR1。SBRMLSS=1.8g/L,SRT=20d。1.3　,、、NH4Cl、KH2PO4,1。CODHACH-COD,PHB[4],《》()。1　COD/mgl-1NH3-N/mgl-1NO3-N/mgl-1TP/mgl-1pH/℃180～27010～250～1.05.2～9.607.5～8.520～262　2.1　CODA/ASBR2.1.1　试验结果　,CODA/ASBR2。2,CODA/ASBR。2　CODCOD/mgl-1COD/mgl-1/mgl-1/mgl-1/mgl-1/mgl-1/%/%30015019.8713.212003498220-30040-7016.543.56l20478872204010.137.682010245281第3期　　　　　　　丁彩娟,等:A/ASBR中PHB转化与反硝化吸磷的关系研究　　　　　　　　　　COD300mg/l,34%,98%;COD220mg/l,50%。COD220mg/l～300mg/l,。2.1.2　结果分析　COD,,COD,,,COD。COD,PHB,PHB,,,,。,,CODPHB。2.2　PHB2.2.1　PHB的转化与反硝化吸磷的定量关系分析　PHB。[5～8]。2　PHB,PHB(2),:ηp=7.05ΔPHB+33.99　　(R2=0.996)ηN=5.94ΔPHB+50.87　　(R2=0.987)　　,,PHB。,PHB3　PHB(3),:ΔN=4.45e0.25ΔPHB　　(R2=0.991)ΔP=2.22e0.25ΔPHB　　(R2=0.981)　　,1mgPHB5.71mgNO-3-N,2.85mgPO3-4-P。,,PHB,,,。,PHB,2mgNO-3-N1mgPO3-4-P,51。2.2.2　缺氧段的外碳源对以PHB为碳源的反硝化除磷脱氮的影响　。,。:。,,NO-3-N,。4。82　　　　　　　　　　　　　　　　　重庆建筑大学学报　　　　　　　　　　　　　第27卷4　4,,;,,,,。,,PHB,。,/,COD,。2.3　PHB5　A/ASBR2.3.1　试验结果　A/A/SBR5、6。56:1)A/ASBRCOD,。COD74%。2)/,。,2.5hNO-3-N。6　A/ASBRPHB,10minNO-3-NPO43--P,36.84mgPO43--P/h66.9mgNO-3-N/h。,3mgPO43--P/Lh,A/OSBR70mgPO43--P/gVSSh[9,11];5.95mgNO-3-N/Lh,50mgNO-3-N/Lh[12]。3)PHB,。,PHB16%7.52%。4),6.47%2.29%,16.72mgPO43--P/gMLSSh。,,,8%,A/ASBR/。2.3.2　结果分析　NO-3-N,,PO43--PNO3--N10。3,PHB。,PHB,。NO3--N,ATP,,。,,,NO-3-N,83第3期　　　　　　　丁彩娟,等:A/ASBR中PHB转化与反硝化吸磷的关系研究　　　　　　　　。,,,。3　1),COD,COD,COD,PHB,,。,COD=220～300mg/l,。2)COD。3),PHB。4)PHB,2mgNO-3-N1mgPO3-4-P。:[1]　CarucciA,KuhniM.MicrobialcompetitionfortheorganicsubstratesanditsimpactonEBPRsystemunderconditionsofchangingcarbonfeed〔J〕.WaterScienceandtechnology,1997,36(12):19-27.[2]　,.〔M〕.:,1998.[3]　KubaT.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem〔J〕.WaterRes1996,30(7):1702-1710.[4]　JianY,JianpingW.MetabolicfluxmodelingofdetoxificationofaceticacidbyRalstoniaeutrophaatslightlyalkalinepHlevels〔J〕.BiotechBioeng,2001,73:458～464.[5]　,,,.〔J〕.,2002,24(3):106-111.[6]　BarnardJ.L.CutPhosphorusandNitrogenwithoutchemicalsremove〔J〕.WaterandWastesEng.,1974,11(7):33-36.[7]　Fukase,T.,Shibata,M.,Miyaji,Y.Studiesonthemechanismofbiologicalphosphorousremoval〔J〕.WaterPollu.tRes.1982,5,309-317.[8]　Levin,G.V.,J.Shapiro.Metabolicuptakeofphosphorousbywastewaterorganism〔M〕.JWPCF,1965,37:800.[9]　HaoX.D.ContributionofP-BacteriainBNRprocessestooveralleffectsontheenvironment〔J〕.WaterSciandTechno,2001,44(1):67-76.[10]　W.J.Ng,S.L.Ong,J.Y.Hu.Denitrifyingphosphorusremovalbyanaerobic/anoxicsequencingbatchreactor〔J〕.WaterScienceandTechnolog,2001,l44(1):187-194.[11]　WachtmeisterA,KubaT,Asludgecharacterizationassayforaerobicanddenitrifyingphosphorusremovingsludge〔J〕.WaterRes1997,31(3):471-478.[12]　KubaT.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem〔J〕.WaterRes1996,30(7):1702-1710.84　　　　　　　　　　　　　　　　　重庆建筑大学学报　　　　　　　　　　　　　第27卷