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ABR1,1＊,1,2,1(1., 150090;2., 150080):(ABR),28LABR,100d,COD(OLR),SRV3-2PBSF820,(SSCP)UPGMA,.,,MLVSS18.0g·L-1、COD2000mg·L-1、HRT39.5h、(35±1)℃,31dABR,COD96%;ABROLR,OLR1.2kg·(m3·d)-16.0kg·(m3·d)-1,ABR,COD98%;OLR,ABR,OLR,ABR,,.:;;(ABR);;;(SSCP):X792 :A :0250-3301(2008)08-2206-08:2007-08-20;:2007-11-07:(863)(2006AA05Z109);(50378025):(1960～),,,,,E-mail:jack0688@sina.com＊,E-mail:ljz6677@163.comCapabilityandBacteriaCommunityAnalysisofanAnaerobicBaffledReactorTreatingSoybeanWastewaterBAOLi-xin1,LIJian-zheng1,CHANGSheng1,HUANGXiao-fei2,RENNan-qi1(1.StateKeyLaboratoryofUrbanWaterResourceandEnvironment,HarbinInstituteofTechnology,Harbin150090,China;2.SchoolofLifeandEnvironmentalScience,HarbinNormalUniversity,Harbin150080,China)Abstract:Capabilityandprocesscharacteristicofanaerobicbaffledreactor(ABR)treatingsoybeanwastewaterwereinvestigatedina4-compartmentABRwithaneffectivevolumeof28L.Duringanoperationperiodof100days,theorganicloadingrate(OLR)increasedbystagesanditsinfluenceonthechemicaloxygendemand(COD)removalefficiencywasresearched.Thebacteriacommunitystructuresinanaerobicactivatedsludgefromdifferentstageswerealsoinvestigatedbysingle-strandconformationpolymorphism(SSCP)withtheeubacteriumuniversalprimersSRV3-2PandBSF820,whilethemicrobialgeneticdistancebeinganalyzedbyUPGMAcommunitiesclusteringmethod.Withaninoculatedaerobicactivatedsludgeof18.0g·L-1intermsofmixedliquorvolatilesuspendsolid(MLVSS),thereactorstartedupatCODconcentrationof2000mgL,hydraulicretentiontime(HRT)of39.5handtemperatureof(35±1)℃for31d,theABRachievedastablestatethatresultedin96%CODremoval.WhenOLRincreasedstagebystagefrom1.2kg·(m3·d)-1to6.0kg·(m3·d)-1,thereactorcouldperformedsteadilywithaCODremovalefficiencyashighas98%,andthisindicatedthatcompartmentalizedABRheldagoodperformanceduringshockloadings.ItwasfoundthatastepchangeinOLRhadaremarkablyeffectonthestructureanddistributionofmicrobialcommunitiesineachcompartment.Withtheorganicloadingrateincrease,thegeneticdistancesamongthemicrobialcommunitiesinthecompartmentsextendedgradually,indicatingthatthespecificityofmicrobialcommunitiesineachcompartmentwasenhanced.Keywords:soybeanwastewater;anaerobicdigestion;anaerobicbaffledreactor(ABR);organicloadingrate;microorganismcommunity;single-strandconformationpolymorphism(SSCP) (anaerobicbaffledreactor,ABR)(stagedmulti-phaseanaerobicreactor,SMPAR)3[1],,,,,.ABR,,,[2],29820088 ENVIRONMENTALSCIENCEVol.29,No.8Aug.,2008DOI:10.13227/j.hjkx.2008.08.019.ABR、、、、[2,3],、、、、、、、、[4～14],[15],,.,[16,17],.、、、[18],,[19].4ABR,,COD,(single-strandconformationpolymorphism,SSCP),,.1 1.1 ABRABR,62cm,56cm,10cm,28L.4,11,2cm,8cm.,4,,.ABR,(35±1)℃.ABR1. 1 ABRFig.1 Schematicdiagramoftheanaerobicbaffledreactor(ABR),COD8000mg·L-1,(CODBOD,CB)0.6,、,.,,;,MLVSS18.0g·L-1.ABR(HRT)39.5h、(35±1)℃、COD2000mg·L-1;,HRT、,COD,(OLR),.,COD4.1(31d),,COD2000mg·L-1,OLR1.2kg·(m3·d)-1;2(32～64d),COD5000mg·L-1,OLR3.0kg·(m3·d)-1;3(65～82d),COD8000mg·L-1,OLR4.8kg·(m3·d)-1;4(83～100d),COD10000mg·L-1,OLR6.0kg·(m3·d)-1.,.,,ABRpH、(ALK)、、COD、.,pH,4.1～5.0.1.2 COD、pHMLVSS,[20],ALKCaCO3;,(VFAs)SP-6890(),.1.3 SSCPABR,.DNA(),DNA.PCR(polymerasechainreaction)SRV3-2P(5′-TTACCGCGGCTGCTGGCA-3′)BSF820(5′-AGAGTTTGATCCTGGCTCAG-3′)[21～23],Invitrogen().PCR()PCR,9700PCR(PE)[24～26];22078:ABRPowerPac1000(Bio-Rad,CA,USA),Bassam[27];SSCPUMAXPowerlook1000(TX,USA),Gel-Pro(MediaCybernetics,MD,USA),UPGMA[28].2 2.1 ABR2COD、pH,100dABR.41.ABROLR1.2 2 ABRCOD、COD、pHFig.2 CODconcentration,CODremovalrate,biogasyieldandpHoftheABRsystemduringitsoperation2208 29kg·(m3·d)-11[2(a)],,COD[2(b)],COD2000mg·L-1,(4)COD2d61mg·L-1,COD97%.ABR,,,COD,6dCOD572mg·L-1,COD71%.,,ABRCOD,23d,ABR1.23～31d(1),ABRCOD820、293、151、87mg·L-1,COD61%、86%、93%、96%,pH6.0、6.8、7.0、7.1,10.4、5.4、2.1、0.5L·d-1,VFAs()567、452、180106mg·L-1.MLVSS18.0g·L-1,20.5、22.8、22.9、19.8g·L-1.ABRCOD、VFAs,CODpH,,.1 ABR4Table1 InfluentCOD,CODremoval,biogasyield,pH,VFAsandbiomassineachcompartmentoftheABRsystemduringitsoperationhoursdCODmg·L-1COD%L·d-1pHVFAsmg·L-1MLVSSg·L-1123～31820±1561±4.3810.4±0.266.0±0.05567±6120.51(1～31d)223～31293±4986±2.915.4±0.226.8±0.08452±4722.8OLR1.2kg·(m3·d)-1323～31151±4093±1.752.1±0.037.0±0.14180±3822.9423～3187±2296±0.840.5±0.037.1±0.09106±2819.8158～642698±40545±5.9233.8±0.485.5±0.061417±15527.12(32～64d)258～64559±10588±2.1518.9±0.736.8±0.05930±8225.3OLR3.0kg·(m3·d)-1357～64180±2196±0.363.4±0.387.2±0.02204±3325.2454～64116±2197±0.440.7±0.317.2±0.06109±2420.6177～823390±24657±2.5750.2±0.085.4±0.012040±14829.53(65～82d)276～82947±15388±2.1421.0±0.337.2±0.021277±6326.6OLR4.8kg·(m3·d)-1375～82479±6394±0.873.5±0.087.3±0.03206±3427.9475～82259±6597±0.831.0±0.127.4±0.03108±2822.8195～1003525±11664±0.6172.9±0.445.3±0.012550±9833.64(83～100d)294～100727±12693±1.2830.1±0.867.2±0.031484±6730.6OLR6.0kg·(m3·d)-1394～100408±9996±0.978.6±0.287.3±0.06192±1632.0492～100224±5398±0.551.6±0.267.3±0.0299±1923.8 33d,ABROLR3.0kg·(m3·d)-12.COD2000mg·L-15000mg·L-1,COD、COD、pH,,58d2(2).COD、、pHVFAs,2(1).454d,54～64d,COD、COD、、pHVFAs116mg·L-1、97%、0.7L·d-1、7.2109mg·L-1.,3、2157、58、58d.357～64d,COD、COD、、pHVFAs180mg·L-1、96%