ABR郑理慎1,2,陈志平1,2,方卫1,2,林培真1,2,邱飞1,2,孙国萍1,2* (1.,510070;2.,510070) 利用ABR-HBR工艺对广州大田山垃圾渗滤液生物处理系统进行改造。结果表明,ABR可有效提高垃圾渗滤液的可降解性,进水BOD5/COD为0.1时,出水BOD5/COD值提高到0.3左右。通过控制回流比在ABR中实现良好的厌氧氨氧化功能,当进水氨氮浓度为427~543mg/L时,出水氨氮浓度降至315~443mg/L,氨氮的去除率达25%;利用探针对ABR厌氧污泥进行原位杂交,结果显示,ABR厌氧污泥中有大量氨氧化细菌。工程运行240d后,在ABR中观察到厌氧颗粒污泥,颗粒污泥的产甲烷活性较同格中絮状污泥提高82.2%。ABR各格中颗粒污泥的粒径大小存在差异,中间格中的颗粒污泥粒径较大,平均为1.20mm,两端格中颗粒污泥的粒径平均为1.02mm。 垃圾渗滤液;ABR;颗粒污泥;厌氧氨氧化 X705 A 0517-6611(2009)14-06577-04StudyonTreatmentofLandfillLeachateandItsGranularSludgewithABRZHENGLi-shenetal (GuangdongNanfangEnvironmentProtectionandBio-technologyLimitedCompany,Guangzhou,Guangdong510070)Abstract ThebiologicaltreatmentsystemonlandfillleachateinGuangzhouDatianshanwasremouldedwithABR-HBRtechniques.There-sultsshowedthatABRcouldeffectivelyimprovethedegradabilityoflandfillleachate.WhentheBOD5/CODvalueofinfluentwas0.1,theBOD5/CODvalueofeffluentwasincreasedto.about0.3.TheanaerobicammoniumoxidationfunctionwasrealizedinABRthroughcontrol-lingrefluxratio.Whenammoniaconcn.ofinfluentwas427-543mg/L,theammoniaconcn.ofeffluentwasdecreasedto315~443mg/L,theremovalratereached25%.TheinsituhybridizationonanaerobicsludgeinABRwasconducted,andtheresultsshowedthattherewereplentyofammonia-oxidizingbacteriainanaerobicsludgeinABR.After240doftheengineeringoperating,theanaerobicgranularsludgewasobservedinABR,andthemethanogenicactivityofgranularsludgewas82.2%higherthanthatofflocculentsludgeinthesamelattice.TheparticlesizeofanaerobicgranularsludgeinlatticesofABRwasdifferent,thatinmiddlelatticeswasbigger,beingaverageof1.20mmandthatinamphioflatticeswasaverageof1.02mm.Keywords Landfillleachate;ABR;Granularsludge;Anaerobicammoniumoxidation 郑理慎(1974-),男,安徽宿松人,学士,从事环保技术和设备方面的研究。*通讯作者。 2009-02-11 (ABR)McCarty1981。,,,,。ABR,,。ABR、、、,。ABR,[1]。ABR,。1 1.1 1989,200210。,。:COD3014~19600mg/L,BOD5300~1500mg/L,NH31129~3264mg/L,P0.8~21mg/L。,C/N;,BOD5/COD0.1,,;,,,100~200t/d。1.2 [2] [2],。1.2.1 。,C/N,。,,,。1.2.2 ABR。C/N、BOD5/COD、,15,ABR。ABR,,,。1.2.3 HBR。,。、,5,HBR。,。:1 Fig.1 Technologicalprocessafterreformation90d,,JournalofAnhuiAgri.Sci.2009,37(14):6577-6580 常俊香 张士敏DOI:10.13989/j.cnki.0517-6611.2009.14.00925mg/L;COD600~800mg/L,COD300mg/L。500t/d,《》(GB16889-1997)。ABR。2 2.1 [3],、,8g/L。2.2 ,,,LECIA。0.24mm。2.3 (fish) Amx820(ROX,),[4]。2.4 CODCr;BOD5BOD5(BODTrakTM,HACHCom-pany);,MLSS、MLVSS。3 3.1 ABRCOD C/N,,COD。,ABRCOD,,。2,ABRCOD,,COD2105~3647mg/L,31%。ABR,BOD5/COD0.10.3(1),。ABR。ABRHBR,COD,150mg/L。,ABR。ABRHBR,,。ABRGC-MS,ABR,。1 ABR、BOD5/CODTable1 BOD5/CODratioofABRinfluentandeffluentDeterminationtimesBOD5/CODInfluentBOD5/CODBOD5/CODEffluentBOD5/COD10.110.2820.130.3130.120.2640.090.313.2 ABR NH+4N、NO-2N[5-6],N2。HBRABR。NO-2、NO-3,2 ABRCODFig.2 TheeffectofABRonCODremoval3 ABRNH3Fig.3 TheeffectofABRonNH3removalNH3,。ABR,427~543mg/L,ABR,315~443mg/L,ABR25%;NO-2、NO-3。,(4、5)。。4 ABRFig.4 FISHresultsofANAMMOXbyactivatedsludge3.3 ABR ABR,ABR。96ABR,30dABR,30d。,,,;6578 安徽农业科学 20095 ABRFig.5 FISHresultsofANAMMOXbygranularsludge。,,。ABR,2,1,。、BOD5/COD,。ABR1,,2,1;,ABR。ABR10.9~27.8mg/L。6 ABRFig.6 SludgeconcentrationinABR7 ABRFig.7 ChangeofsludgemethanogenicactivityinABR3.4 ABR ABR,ABR30d,ABR,30d,。ABR。ABR,,ABR[1,7-8],ABR,。。,“”,,。,,。ABR1#,410ml/(g.VSS.d),747ml/(g.VSS.d),82.2%。8 Fig.8 Comparisonofmethanogenicactivityofgranularandflocculentsludge9 ABRFig.9 DistributionofgranularsludgediameterintheABR3.5 240d,ABR(10)。,,15,,。,4:、、。VSS/MLSS65%~75%,VSS/MLSS。,Ca2+,Ca2+CO2CaCO3,。15,Ca2+,CaCO3,VSS/MLSS。ABR,0.6~0.89mm,22%~28%。ABR3,1.20mm,6579 37卷14期 郑理慎等 ABR处理垃圾渗滤液及其颗粒污泥的研究2,1.11mm。1、4、51.03、1.02、1.02mm。[9]13.2L4ABR,ABR,ABR,,。。10 Fig.10 Microscopicimageofgranularsludge3.6 ABR 11,,,。0.63mm15m/h,。0.64~1.59mm37m/h,1.60~1.99mm51m/h。2mm70m/h,[10]。:10.23~0.31mm;20.32~0.63mm;30.64~1.6mm;41.6~1.9mm;52.0mm。Note:1.Granularsludgediameteris0.23-0.31mm;2.Granularsludgedi-ameteris0.32-0.63mm;3.Granularsludgediameteris0.64-1.6mm;4.Granularsludgediameteris1.6-1.9mm;5.Granularsludgediameterisover2.0mm.11 Fig.11 Settlementrateofdifferentgranularsludgediameters4 ABR,NH+4、BOD5/COD,、、HRT,。ABR:①,、,。②ABR,427~543mg/L,315~443mg/L,25%。③ABR,30d,10.9~27.8g/L。④。。⑤ABR,1.20mm,,1.02mm。[1]BARBERWP,STUCKEYDC.Theuseoftheanaerobicbaffledreactor(ABR)forwastewatertreatment:areview[J].WaterRes,1999,33(7):1559-1578.[2]许玫英,方卫,张丽娟,等.生物脱氮新技术在垃圾渗滤液工程化处理中的应用[J].环境科学,2007,3(28):607-612.[3]贺延龄.废水的厌氧生物处理[M].北京:中国轻工业出版社,1998.[4]AMANNRI.Insituidentificationofmicroorganismsbywholecellhybriza-tionwithrRNAtargetednucleicacidprobes[M].Dordrecht:KluwerAca-demicPublishers,1995:1-15.[5]VANDEGRAAFAA,MULDERA,DEBRUIJNP,etal.Anaerobicoxida-tionofammoniumisabiologicallymediatedprocess[J].ApplEnvironMicrobiol,1995,61(4):1246-1251.[6]VANDEGRAAFAA,DEBRUIJNP,ROBERTSONLA,etal.Autotroph-licgrowthofanaerobicammonium-oxidizingmicro-organismsinafluidizedbedreactor[J].Microbiology,1996,142(8):2187-2196.[7]LETTINGAG,FIELDJ,ZEEMANG.Advancedanaerobicwastewatertreatmentinthenearfuture[J].WaterScienceandTechnology,1997,35(10):5-12.[8]郭晓磊,胡勇有,高孔荣.厌氧颗粒污泥及其形成机理[J].给水排水,2000,26(1):33-38.[9]沈耀良,