AmOn工艺中悬浮填料填充率对硝化菌群的影响王峰

:2007-01-07:(2006BAJ04A00);“”(2004AA649310):　(1978—),,,.E-mail:[e-mail]:(1971—),,,,,.E-mail:[e-mail]王　峰1,刘长青2,刘　易1,张亚雷1(1.,200092;2.,266033):AmOn.(AOB)(NOB).AOB(βProteobacteria)NOB(NitrospiraNitrobacter)16SrDNA,(PCR),(DGGE);.,AOBNitrobacter40%50%,AOBNOB40%30%.AmOn.AmOn,NitrosomonasAOB.NOB,NitrospiraNitrobacter.:;AmOn;;;;:X172　　　　　　:A　　　　:0253-374X(2008)08-1085-04InfluenceofPackingPercentagesonNitrifyingBacteriaCommunityinAmOnProcessWANGFeng1,LIUChangqing2,LIUYi1,ZHANGYalei1(1.StateKeyLaboratoryofPollutionControlandResourceReuse,TongjiUniversity,Shanghai200092,China;2.InstituteofEnvironmentandMinicipalEngineering,QingdaoTechnologicalUniversity,Qingdao266033,China)Abstract:TheAmOnprocessisanewtypeofhighlycombinedprocessforwastewatertreatment.Inthisstudy,moleculartechniqueisappliedtotheinvestigationofthecommunitystructureandbiodiversitylevelofammoniaoxidizingbacteria(AOB)andnitriteoxidizingbacteria(NOB)underdifferentpackingpercentagesinAmOnreactor.Nestedpolymerasechainreaction(PCR)wascarriedouttoamplifyspecific16SrDNAfragmentofAOB(β-Proteobacteria)andNOB(includingNitrospiraandNitrobacter),andthePCRproducewassep-aratedbydenaturinggradientgelelectrophoresis(DGGE)method.ThebiodiversitywascalculatedonthebasisofShannonIndex.TheresultshowsthatAOBandNitrobacterpopulationshavesimilarcommunitystructuresatpackingpercentagesof40%and50%.Allnitrifyingbacterialpopulationsshowobviouschangewhenthepackingpercentageisadjustedfrom40%to30%.Evenfedwiththesameinfluent,differenttypesoftreat-mentprocessesalsohavespecificdominantspecies.InAmOnreactor,NitrosomonasandNitrospiraaredomi-nantAOBandNOBgenus,respectively.Keywords:wastewatertreatment;AmOnprocess;ammoniaoxidizingbacteria;nitriteoxidizingbac-teria;denaturinggradientgelelectrophoresis;packingpercentage36820088()JOURNALOFTONGJIUNIVERSITY(NATURALSCIENCE)Vol.36No.8　Aug.2008　　AmOn.、,,,,.,,,,[1].,,.,[2].AmOn,,,.,,.,,.PCR(nestedpolymerasechainreac-tion,)DGGE(denaturinggradi-entgelglectrophoresis,)AOB(ammoniaoxidizingbacteria,)NOB(nitriteoxidizingbacteria,),,.1　1.1　AmOn1,,,.,,.:(),、.,,,.,,;,.1　AmOnFig.1　FlowschematicofAmOnprocess　　,.,50%,40%30%().30%().().1.2　,..30%,10mL,(6000r·min-1,4℃)5min.[3].1.3　PCRDGGEDGGEPCR1.PCR16SrDNA.:AOB,NOBNitrospiraNitrobacter,1%.DGGEBioRadDCode.AOBNitrospira()25%～50%,Nitrobacter45%～65%.[8].1.4　DGGE(Shan-nondiversityindex)H,H=-∑(ni/N)lg(ni/N):ni;N.(clusteringanalysis)SPSS11.0.1086　　　()36　1　DGGEPCRTab.1　PrimersandprogramsfornestedPCRamplificationfollowedbyDGGEPCR1)AOBCTO189fAB/CTO189fC,CTO653r95℃,1min;63℃,1min;72℃,2min[4]NitrospiraP63f,NSR1264r95℃,1min;60℃,1min;72℃,2min[5-6]NitrobacterP338f,Nb1000r95℃,1min;58℃,1min;72℃,2min[5]P338f-gc,P518r95℃,1min;60℃,1min;72℃,2min[7]　　1)95℃,10min;72℃,10min;35.2　2.1　,2.(NH+4—N)(TN).50%30%,91.1%60.0%64.8%49.7%.,.30%,,.2　Fig.2　Performanceofdenitrificationandnitrification2.2　DGGE3.,.50%40%,30%,AOB.30%,AOB1,AOB2,AOB3AOB9..AmOnAOB,AOB7AOB8.AmOnAOB,AOB7AOB8.,AmOn,.1—50%;2—40%;3—30%;4—30%;5—3　DGGEFig.3　DGGEprofilesinsludgeandbiofilmondifferentcondition　　NitrospiraNitrobacter,AmOn.,Nitrobacter40%,50%,Nb1,Nb2Nb3.30%,Nitrobacter,Nb2,Nb430%,,.NitrospiraAmOn,.,30%,AmOn,Ns1Ns5,Nitrospira.1087　8　,:AmOn　　2.3　4.,AOBNitrospira.Nitrobacter.AOBNi-trospira.,,,,.4　Fig.4　Changeinbiodiversityindexofnitrifyingbacteriacommunity　　Schramm,NitrobacterNOB[9].,[10].,NOBNitrospira[11-12].,NitrobacterNitrospira、.,Nitrobacter,Nitrospira,NitrospiraAmOnNOB.NOB,AOBNi-trosomonasNitrosococcus,[11-14].AOB16SrDNA,AOB,AOB7AOB8Ni-trosococcus;6Nitro-somonas.3　PCRDGGEAmOn.,AmOn,AOBNitrosomonasNi-trosococcus,Nitrosomonas.NOB;NitrospiraNitrobacter,NitrospiraNOB...,AOBNitrobacter,Nitrospira.40%30%.AmOn..,AmOn.:[1]　,,,.AmOn[J].,2005,25(1):72.　　　ZHANGYalei,ZHAOJianfu,WUYong,etal.DevelopmentofintegrativeAmOnbioreactorforwastewatertreatment[J].ChinaWaterandWastewater,2005,25(1):72.[2]　,,,.AmOn[J].,2006,32(12):71.　　　LIUChangqing,ZHANGFeng,ZHANGYalei,etal.IntegratedAmOnprocessformunicipalwastewatertreatment[J].Technol-ogyofWaterTreatment,2006,32(12):71.[3]　ZhouJ,BrunsMA,TiedjeJM.DNArecoveryfromsoilsofdi-versecomposition[J].AppliedandEnvironmentalMicrobiology,1996,62(2):316.[4]　KowalchukGA,StephenJR,BoerWD,etal.Analysisofammonia-oxidizingbacteriaoftheβsubdivisionoftheclasspro-teobacteriaincoastalsanddunesbydenaturinggradientgelelec-trophoresisandsequencingofPCR-Amplified16SRibosomalDNAfragments[J].AppliedandEnvironmentalMicrobiology,1997,63(4):1489.[5]　MobarryBK,WagnerM,UrbainV,etal.Phylogeneticprobesforanalyzingabundanceandspatialorganizationofnitrifyingbac-teria[J].AppliedandEnvironmentalMicrobiology,1996,62(6):2156.[6]　RobinsonKG,DionisiHM,HarmsG,etal.Molecularassess-mentofammonia-andnitrite-oxidizingbacteriainfull-scaleacti-vatedsludgewastewatertreatmentplants[J].WaterScienceandTechnology,2003,48(8):119.(1106)1088　　　()36　4　28dFig.4　Testingresultsofholestructureinthecomparisonmortarsamplesattheageof28d;(),CK/(1+K)1/2,.()(),,Aft()、C—S—H,,.:[1]　TANKefeng,PUXincheng.Strengtheningeffectsoffinelygroundflyash,granulatedblastfurnacesla