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71Vol．7No．120131ChineseJournalofEnvironmentalEngineeringJan．2013A-O710048A-OCOD。ATPCOD。ATP0.38～0.77mg/L84%COD70.01%ATP0.86～2.17mg/L75%COD96.2%。ATPCODX79A1673-9108201301-0258-05RelationshipbetweensludgehydrophobicratioandsludgeactivityinA-OprocessGaoZhanyaoTongPanruiLiHongjiSchoolofEnvironmentalandChemicalEngineeringXi’anPolytechnicUniversityXi’an710048ChinaAbstractPrintinganddyeingwastewaterwasdegradedthroughA-OprocessThemicrobialratioofhydro-phobicandCODremovalchangeunderdifferentmicrobialscantywereresearched．TheresultsshowthattherearethecertainrelationsbetweentheATPconcentrationasthesludgeactivityindexandthemicrobialhydropho-bictheyaffecttheCODremoval．WhenATPconcentrationsastheactivityindexofhypoxiasludgearebetween0.38mg/Land0.77mg/Lthefirst-rateofthehydrophobicityofthesludgemicroorganismis84%andthehighestCODremovalrateis70.01%．WhenATPconcentrationsastheaerobicactivatedsludgeindexarebe-tween0.86mg/Land2.17mg/Lthefirst-rateofthehydrophobicityofthesludgemicroorganismis75%andthehighestCODremovalrateis96.2%．KeywordsactivatedsludgeATPratioofhydrophobicitychemicaloxygendemandCODsludgesurfaceproperties2011－07－152011－10－191985～。E-mailgaozhanyao607@163.com1-3Mudd19244。。5。ATP。ATP。ATP/ATP。ATP。ATP、、ATP、。ATP。ATP6。1A-O、DNA、7-10。ATP、11。ATP、、12-14。15。ATP。ATPCODA-O。11.1A-O70L30L。1000mg/LNH4Cl100mg/LKH2PO420mg/LNa2CO3300mg/L。CaCl210mg/LMgSO45mg/LMnSO45mg/LFeSO45mg/L。2～3h。MLSS9.52g/L16。35L12L。。20d20dCOD279.5～421.1mg/L50%COD80%～90%70%。。HRT14hHRT8h0.5mg/L2～4mg/L。1.21.2.1试剂制备1TrispH=7.82.422gTris=121.141000mL。2ATPATP-Na2C10H14N5O13Na2·3H2O=605。1.2.2ATP提取与测定1ATP。、、310mL10mL10mLTris10min3000r/min5～10min。2ATP。0.605gATP1000mLTrisATPTris0.000945～0.0605g/L。1ATPFig.1ATPstandardcurve3ATP。1260nmy=24.264x－0.0106ATP。1.2.3微生物疏水性测定1-17。600nmA。CAA=kCk1．9527r=C0－C/C0×100=A0－A/A0×100%2rC0、C/mLA0、A。2。。30min600nm。26∶4。。、1.5∶=6mL∶4mL。2Fig.2Relationshipbetweenextractingagentdosageandmicrobialspectrophotometry3。、、310mL10mL4000r/min30min31。600nmA06mL4mL20s20min1000r/min10min600nmA1A0A12r。22.1ATP3ATP0.38mg/LATP0.38～0.77mg/L64%～84%84%ATPATP0.89mg/L46.9%。7ATP。0.5mg/L0.2mg/LATP0.38mg/L1mg/LATP0.77mg/LATP。3ATPFig.3RelationcurvesofATPconcentrationandmicrobialscantywaterratioinhydrolysisacidificationpool2.2ATP4ATP0.86mg/LATP0.86～2.17mg/L65%～75%ATP2.27mg/L35.1%ATP。ATP0.86～2.17mg/L。ATP0.86mg/LATP2.17mg/LpH0621A-OATP。4ATPFig.4RelationcurvesofATPconcentrationandmicrobialscantywaterratioinaerobicaerationpool2.3、ATPCOD5ATP0.38～0.57mg/LCOD0.57～0.78mg/LCOD65.29～70.01%70.01%。5ATP、CODFig.5RelationcurvesofATPconcentrationmicrobialscantywaterratioandCODremovalrateinhydrolysisacidificationpool6ATPCODATPATP0.86mg/LCODATP0.86～2.17mg/LCOD87%～96%ATP2.27mg/LCOD81.7%。6ATP、、CODFig.6RelationcurvesofATPconcentrationmicrobialscantywaterratioandCODremovalrateinaerobicaerationpoolATP。5、6ATPCODCODCODCOD。18。5、6ATP0.38～0.57mg/LATP0.86～2.17mg/L。31、1.5∶=6mL∶4mL。2ATP0.38～0.77mg/L64%～84%84%COD70.01%。31627ATP0.86～2.17mg/L65%～75%75%COD96.2%。4A-OATP0.38～0.57mg/LATP0.86～2.17mg/L。1HermanssonM．JlangQ．GotsehalkB．W．Hydrop-hobicandelectrostaticcharacterizationofbacteriaanditsrela-tionshiptoadhensiontoanairwaterinterfaces．ArchMiero-biol．198313308-3122VanLoosdrechtMCMLyklemaJ．NordeW．etal.Theroleofbacterialcellwallhydrophobicityinadhension．Ap-pl．Environ．Microbiol．19875381893-18973DevasiaP．SurfacechemistryofThiobacillusf-errooxidousrelevanttoadhensiononmineralsurfaces．Appl．Environ．Microbio1.199359124051-40554RosenbergM．KjellebergS．HydrophobicinteractionsRoleinbeacterialadhension．K．C．AdvancesinMicrobialEcolo-gy．NewYorkPlenumPress1986．353-3935．ATP．20072437-11YinJ．SangL．LiL．StatusofApplicationandstudyofATPmeasurementinactivatedsludgeprocess．JournalofJi-linArchitecturalandCivilEngineeringInstitute20072437-11inChinese6．．20101381-382HenD．WangF．Activatedsludgeactivitydetectionmeth-ods．ScienceandTechnologyInformation20101381-382inChinese7．．199314258-62XuX．L．ShenX．Y．Activatedsludgeactivityparame-tersselectionandevaluation．EnviromentalScience199314258-62inChinese8．TTC-．201047SongD．J．TTCdehydrogenaseactivitydeter-minationofsewagesludgeactivity．HeilongjiangScienceandTechnolo-gyInformation201047inChinese9．PCR·DGGEDNA．2011391101-102GaoH．Q．LiuL．ReviewofdifferentDNAextractionmethodsinPCR-DGGEtechnique．JournalofAnhuiAgri．Sci．2011391101-102inChinese10．pH．2007331262-65LiJ．X．QiaoL．ZhaoJihongetal．ApplicaionofsludgeactivitymeasurementbypHvaluemethodinengi-neering．TechnologyofWaterTreatment2007331262-65inChinese11．．200293656-58HongM．ApplicationofATPdeterminationinwastewatertreatmentplants．ChemicalEngineer200293656-58inChinese12．ATP．2008209124-125WuJ．ZhuH．H．Researchoneffectofquaternaryammo-niumsurfactantsinextractofATP．ActaAgriculturaeJiangxi2008209124-125inChinese13．ATP．1992313-1814．ATP．200317230-33WangL．Y．FanL．H．XiaoA．F．etal．ReviewonthemeasurementmethodsofATP．TianjinChemicalIndus-try．200317230-33inChinese15．pH．20082121599-1604ZhuZ．LiT．WangD．S．etal．EffectofpHonsur-facepropertiesandphysicalstructureofactivatedsludgeflocs．ChineseJournalofEnvironmentalEngineering20082121599-1604inChinese16．--．200020833-36ZhuS．ZWangZ．C．XuQ．Y．