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26520065ActaScientiaeCircumstantiaeVol.26,No.5May,2006:-()(No.50521140075);(No.20377003);SupportedbytheNationalNaturalScienceFoundationofChina(No.20377003);TheKeyInternationalCooperativeProjectofNSFC(No.50521140075);TheOpenProjectofKeyLaboratoryofBeijing:(1976),,;3(),E2mail:pyz@bjut.edu.cnBiography:MAYong(1976),male,Ph.D.;3Correspondingauthor,E2mail:pyz@bjut.edu.cn,,,.2006.A/O()[J].,26(5):703-709MaY,WangSY,ZengW,etal.2006.A/Opilot2scalenitrogenremovalprocesstreatingdomesticwastewater.Thestudyofshort2cutnitrificationanddenitrification[J].ActaScientiaeCircumstantiae,26(5):703-709A/O()1,2,2,1,2,3,31.,,1500902.,,10002231,266033:2005208219:2006203221:2006203221:A/O,pH(MLSS)(FA)(SRT)(DO)(HRT)A/O,DOA/O,FISHDO(0.30.7mgL-1)(NOB),,85%,95%DO,185%10%,DO,2(75%)DO,,,,,SVI80120mLg-1:A/O;;;DO,;FISH:025322468(2006)0520703207:X70311:AA/Opilot2scalenitrogenremovalprocesstreatingdomesticwastewater.Thestudyofshort2cutnitrificationanddenitrificationMAYong1,ZENGWei2,WANGShuying2,PENGYongzhen1,2,31.SchoolofMunicipalandEnvironmentalEngineering,HarbininstituteofTechnology,Harbin1500902.KeyLaboratoryofBeijingforWaterEnvironmentalRecoveryEngineering,BeijingUniversityofTechnology,Beijing1000223.InstituteofEnvironment&MunicipalEngineering,QingdaotechnologicalUniversity,Qingdao266033Received19August2005;receivedinrevisedform21March2006;accepted21March2006Abstract:Theeffectofmainfactors,suchaspH,MLSS,FA,T,SRT,DOandHRT,onshort2cutnitrificationanddenitrificationwassystematicallyanalyzedinapilot2scaleA/Onitrogenremovalplanttreatingdomesticwastewater.TheexperimentalresultsdemonstratedthatDOisthemainfactortoachieveshort2cutnitrificationinthestudy.Fishanalysispresentedthatalong2termoperationatlowDO(0.30.7mgL-1)concentrationleadtotheeliminationofammoniaoxidizingbacteria(AOB),soastorealizenitriteaccumulation.Theaveragenitriteaccumulationratiocouldreach85%;sometimesthenitriteaccumulationratiowashigherthan95%.Theshort2cutnitrificationanddenitrificationwasdamagedwiththenitriteaccumulationratiodecreasingfrom85%to10%whenDOwasincreasedabove1.5mgL-1.Thenitriteaccumulationratiowasresumedto75%afterabouttwosludgeageswiththeDOkeptinlowlevel.TheSVIincreasedwiththeincreaseofnitriteaccumulationratiointheinitialperiod,buttheSVImaintainedataproperlevelof80120mLg-1intheaftertimenomatterhowhighorlowthenitriteaccumulationratiowas.Keywords:A/Opilotplant;domesticwastewater;short2cutnitrificationanddenitrification;DO;nitriteaccumulation.(Nitrosomonas)NH+42N26NO-22N;(Nitrobacter)NO-22NNO-32N.NO-32NNO-22NNON2ON2,NO-22N.Voets(1975),NO-22N,.,,NH+42NNO-22NN2,.,,(TurkandMavinci,1987):1)25%,;2)40%,;3)NO-22NNO-32N63%;4)50%;5)30%40%;6).pH,,.,,,NO-22N.,,:(FA)(Surmaczetal.,1997)pH(Villaverdeetal.,1997)(25)(Hellingaetal.,1998)DO(Mnchetal.,1996)(TurkandMavinic,1986)(FH)(YangandAlleman,1982).SBR,,,DOpHFA.95%,.,(10L),,A/O(Ruizaetal.,2003;Ciudadetal.,2005).,A/O,,A/O,,,,A/O.1(Materialsandmethods)1.1,(1).300L.8().2,6.,100L.,2022,30004000mgL-1,SRT15d,2,0.5,650800Ld-1,HRT911h,DO,ORPDOpH.1A/OFig.1TheschematicdiagramofA/Opilotplant1.2,1,COD248.6mgL-1,(TN)84.9mgL-1,C/N,2.93.4075:A/O()1Table1TheinfluentcharacteristicspHCOD/(mgL-1)TN/(mgL-1)NH+42N/(mg.L-1)NO-22N/(mgL-1)NO-32N/(mgL-1)/(mgL-1)7.157.70163.2389.068.5110.265.4105.70.050.250.691.832004207.50248.684.979.10.151.183201.3CODTNNH+42NNO-32NNO-22NPO-42PMLSS,.DOWTW2level2,pHORPWTW340i.2(Experimentresults)2.12,A/O,(,=(NO-22N)100%/((NO-22N)+(NO-32N))4.3120%31580%(),,95%(315425),(42552),15%30%(53521),20%70%(521526),,,80%.:----,,:FApHSRTDOHRTMLSS,A/O.2Fig.2Thenitriteaccumulationatdifferentphase2.2A/O2.2.1Balmelle(1992)1020,,FA,,,FA.2025,.25,FA,FA.,SHARON,3036(Hellingaetal.,1998).(2001)SBR28,(19.523.5),.2022,,,.2.2.2pHpH,pH,pH,,pH7.08.5,6.07.5.(1999),pH7.4813,,pH8.0,pH7.0.pH7,,.pH,pH.3A/OpH.,pH,7.27.7.,pH,.,,pH,,pH.507263pHFig.3TherelationbetweentheinfluentpHandnitriteaccumulation2.2.3FA(FA),pH,.FA(Daniel,1998):A=1714B10pHKb/Kw+10pH=1.214B10pHe6344/T+10pH,A,mgL-1;B,mgL-1;Kb;Kw;T,K.FA,FA,FA,FA.4FA,FA0.41.5mgL-1,0.93mgL-1.,FA0.150.70mgL-1,0.41mgL-1.Abelin(1992)FA15mgL-1,.Balmelle(1992)FA(1mgL-1).,FA1.0mgL-1,,,FA,FA0.75mgL-1.FA1.0mgL-1,FA(TurkandMavinci,1986;1987;Mauretetal.,1996).,FA,,FA,,FA.4FAFig.4TherelationbetweennitriteaccumulationandtheFAinrawwastewaterandtheinfluentofaerobiczone2.2.4SRTHRTMLSS,30004000mgL-1,3500mgL-1.,.,,,,,MLSS.SRT,(Hellingaetal.,1998),SHARONSRTHRT,,.Pollice(2002),32pH7.2,,,SRT10d,.SRT15d,,,,SRT,SRT.2.2.5DO5DO,,DO0.30.7mgL-1(0.5mgL-1),,,3320%31580%.1.21.5mgL-10.20.4mgL-1,6075:A/O(),DO,.DO(315410),95%,,100%.DO,,.5DOFig.5TherelationbetweennitriteaccumulationandDO,48,FISH,6,(NOB),(AOB),AOB5%,DONOB,.415DO(DO1.75mgL-1),DO,.415425,DO,,DO,,.425,DO0.5mgL-1,,42685%5140%,10dDO,,DO.6DOAOBNOBFISHFig.6FishanalysisforAOBandNOBattheendaerobiczonesunderlong-termlowDOoperation70726DO(0.30.7mgL-1),(51527)(15%40%),527,53063,75%,85%.,DO,,DO.DO,,,,DO,.2,,,.,,,DO,,,DO,,,.,DO,.CecengGoneng(1994),DOFA5,,.5,.DO0.30.7mgL-1,0.5mgL-1,FA0.41mgL-1,DOFA1.22,5,,DO.2.2.6HRTHRT,HRT,.7,327HRT,,HRT,,,,,HRT,,,,.,328418,HRT9h,,5.HRT.430510HRT,,,HRT9h,,HRT,H