AO系统中活性污泥组成细菌的分离与鉴定林冰

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科研开发,2003,11(4):21~23SCIENCE&TECHNOLOGYINCHEMICALINDUSTRY:2002-08-19: (1967-),,,,。*:A/O。A/O*林 冰,李 冰,马 兰,岳文青,高 杨,刘广秀(, 132021) :通过菌胶团破碎、混合细菌分离纯化、单一菌种生理生化特性研究等工作,从厌氧/好氧(Anoxic/Oxic,简称A/O)工艺活性污泥系统中分离出8种细菌并分别鉴定出其属别,其中普通细菌3种、亚硝化细菌4种和硝化细菌1种。在A/O工艺进水水质出现波动和需要调节出水质量的情况下,参照各种细菌的固有特性,调节和控制生产工艺参数,可迅速而有效地保证和提高A/O工艺处理化工生产废水的效果。:A/O工艺;活性污泥;细菌鉴定;属别:X78  :A  :1008-0511(2003)04-0021-03  A/O。,A/O,NH3-N。,,。,,,,A/O。1 ,,[1],,,。,,。:A/O,,5min,5min,5~8,。2 2.1 1h,,8410-1。2.2 10-4~10-100.1mL,,,36℃。3,、。1。2.3 ,,36℃。DOI:10.16664/j.cnki.issn1008-0511.2003.04.0071 31)/g1616NaCl81600mL30CaCO31.6(NH4)2SO46.6NaCl3.0KH2PO41.4MgSO4·7H2O2.51600mL30CaCO31.6NaNO23.5NaCl3.0KH2PO41.4MgSO4·7H2O2.51600mL30  1),pH6.8~7.2,500mL,,,15MPa、121℃20min,,4℃。,,15~20mL。2.4 ,36℃。3 、。3.1 ,,,0.1mL13,,36℃。、、、、、、、、、。3.2 ,、、、、、,。3.3 。3.3.1 、。;;、。,,,;,,;,,,。32。2 /g1)510NaCl51000mL1)510NaCl51000mL52)100mL2.5%(w)4mL  1)pH=6.8~7.2,,5mL,121℃20min,,4℃。2)pH=6.8~7.2,,10mL,110℃20min,,4℃。3,36℃24h,。3.3.2 、、、,、。3。3 1)/g2)5510NaCl51000mL1.6%1mL  1)pH=6.8~7.2,,5mL,,121℃20min,,4℃。2)、、。,36℃·22·      1124h,。4 、、,8,3,4,1,4。4 8/mm/μm1~3-0.7×1.0--------1~2-0.5×1.0++++----1~2+1.0+-+-+-+-1~3-0.5×1.5--------1~2+1.0--------1~3-0.5×1.0--------1~2+0.8×1.0+-+-+-+-1~2-0.5×1.0--------5   A/O,A/O,A/O,,,,A/O。[   ][1] .[M].:,1989.355~356.[2] .[M].:,1990.5~6.SIEVINGANDAPPRAISINGBACTERIAFROMTHEACTIVATEDSLUDGEPRODUCEDFROMA/OTECHNICALPROCESSLINBing,LIBing,MALan,YUEWen-qing,GAOYang,LIUGuang-xiu(WastewaterTreatmentPlantofJilinChemicalIndustrialCo.,Ltd.,Jilin132021,China)Abstract:Eightspeciesofbacteriaaresievedandappraisedbybacteria-groupfragmentation、bacteriamix-tureliquiddilution、platesievingmethod、physiologicalandbiochemicalcharactersanalysisonindividualspecies.Therearethreespeciesofordinarybacteria、fourspeciesofnitrifyingbacteriaandonespeciesofdenitrifyingbacteriainactivatedsludgeofA/Otechnicalprocess.Attheconditionoffluctuationofinfluentwastewaterqualityorchangingtheoutflowquality,theresultofchemicalwastewaterwhichistreatedthroughA/OtechnicalprocesscanbekeptorimprovedquicklyandefficientlybychangingandcontrollingtheparameterofA/Otechnicalprocessaccordingtothespecialcharactersofvariousbacteria.Keywords:A/Otechnicalprocess;Activatedsludge;Sievingandappraisingbacteria;Speciesofbacteria·23·4 ,.A/O   

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