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29420107ENVIRONMENTALCHEMISTRYVo.l29,No.4July20102009619.*(40971183).**,Emai:lcaibaol@inankaeducn;Te:l02223503617ArthrobacterspAD30PseudomonasspAD39*朱希坤王雪涵姚金城李清艳牛淑敏蔡宝立**(,,300071)ArthrobacterspAD30PseudomonasspAD39,,CODCr1702mgl-1133mgl-1(HRT)24h,CODCr100mgl-1,02mgl-1,(GB215232008),200mgkg-1,3020d,991%,AD30AD39ArthrobacterspAD30,PseudomonasspAD39,,.,(20000t),(244d),(),,,,28[1],11mgg-1[2],,,,atzABCDEF,6NH3CO2,PseudomonasspADP[3]trzNatzBC,3,ArthrobacteraurescensTC1[4],[58],,,,ArthrobacterspAD30PseudomonasspAD39,PseudomonasspADP[9],,11161029ArthrobacterspAD30PseudomonasspAD39[9],PseudomonasspADPLPWackett[10]12(atz,gl-1):KH2PO409,Na2HPO412H2O65,MgSO47H2O02,1ml[11],3(),02(,10gl-1,):30,150rmin-113,022m,HP5,30m02mm025m::90,1min(30min-1);240,3min(5min-1),250,2min;250,290,10mlmin-1,2mlmin-1,60mlmin-1[12]1410g30ml,30min,10000rmin-115min,,022m,,1315AD30AD391,,15L11.;2.;3.;4.;5.;6.Fig1Miniaturebioreactorfortreatmentofatrazinecontainingwastewater,,,,,.,:pH60,CODcr480mgl-1,05%,235mgl-1Na2HPO4(500mgl-1),(133mgl-1),(500mgl-1),CODCr1702mgl-1,48hAD30AD3911,5%,4d,,,(HRT),HRT12h24hCODCr16AD30AD3920mlAD30AD39ADP,09%NaClAD30AD3911,03gl-1(02gNa2HPO412H2O,01gKH2PO4)3gl-1,ADP3gl-103gl-1,108ml-19cm10g2mg1mlAD30AD39,3020dADP,.,01235101520d3,-20221ArthrobacterspAD30PseudomonasspAD394:ArthrobacterspAD30PseudomonasspAD39611AD30AD39,trzNatzBatzC,[9]200mgl-1,3048h,AD30AD39925%979%,,996%,(2),,AD30AD392AD30AD39Fig2CurvesofatrazinebiodegradationofstrainsAD30,AD39andmixedstrains22ArthrobacterspAD30PseudomonasspAD39221,,,,,,,,,4d,222,2025CODCr1702mgl-1,133mgl-1,CODCr(3)HRT12h,4d,,CODCr1075mgl-12738mgl-1,133mgl-104mgl-15d15d,CODCr888%,173mgl-1190mgl-1,150mgl-1(GB215232008[13])037mgl-1045mgl-1(3),5mgl-13HRT12h24hCODCrFig3VariationofCODCrandatrazineconcentrationineffluentforHRT12handHRT24h61229HRT24h,5d,,CODCr1702mgl-195mgl-1,133mgl-1017mgl-1(3)6d15d,,CODCr943%,90mgl-197mgl-1,014mgl-1019mgl-1,CODCrGB215232008,,CODCr150mgl-15mgl-1GB215232008,,CODCr100mgl-180mgl-1,3mgl-11mgl-1,,AD30AD39,23ArthrobacterspAD30PseudomonasspAD39Silva[14]Strong[8],ADPatzA,,AD30AD39(3gl-1)(03gl-1),ADP(3gl-1,)(03gl-1),3020d,AD30AD39200mgkg-119mgkg-1,991%.ADP,,98%(4mgkg-1),200mgkg-1160mgkg-1,20%,(4).,AD30AD394AD30AD39ADPFig4DegradationofatrazineincontaminatedsoilbymixtureofAD30,AD39andADP3(1)ArthrobacterspAD30PseudomonasspAD39,CODCr1702mgl-1133mgl-1,HRT24h,CODCr100mgl-1,02mgl-1,GB215232008(2),200mgkg-1ArthrobacterspAD30PseudomonasspAD39,3020d,991%,[1],,,[J],2008,27(2)5966014:ArthrobacterspAD30PseudomonasspAD39613[2]StruthersJK,JayachandranK,MoormanTB,BiodegradationofAtrazinebyAgrobacteriumradiobacterJ14aandUseofThisStraininBioremediationofContaminatedSoil[J]Appl.Environ.Microbiol.,1998,64(9)33683375[3]MartinezB,TomkinsJ,WackettLPetal,CompleteNucleotideSequenceandOrganizationoftheAtrazineCatabolicPlasmidpADP1fromPseudomonasspStrainADP[J]J.Bacteriol.,2001,183(19)56845697[4]ShapirN,MongodinEF,SadowskyMJetal,EvolutionofCatabolicPathways:GenomicInsightsintoMicrobialsTriazineMetabolism[J]J.Bacteriol.,2007,189(3)674682[5]HerzbergM,DosoretzCG,TarreSetal,SimultaneousRemovalofAtrazineandNitrateUsingaBiologicalGranulatedActivatedCarbon(BGAC)Reactor[J]J.Chem.Technol.Biotechnol.,2004,79(6)626631[6]LiuC,HuangX,WangH,StartupofaMembraneBioreactorBioaugmentedwithGeneticallyEngineeredMicroorganismforEnhancedTreatmentofAtrazineContainingWastewater[J]Desalination,2008,231(13)1219[7]LimaD,VianaP,AndreSetal,EvaluatingaBioremediationToolforAtrazineContaminatedSoilsinOpenSoilMicrocosms:TheEffectivenessofBioaugmentationandBiostimulationApproaches[J]Chemosphere,2009,74(2)187192[8]StrongLC,McTavishH,SadowskyMJetal,FieldScaleRemediationofAtrazineContaminatedSoilUsingRecombinantEscherichiacoliExpressingAtrazineChlorohydrolase[J]EnvironMicrobiol,2000,2(1)9198[9],,,[J],2009,36(7)10991104[10]MandelbaumRT,AllanDL,WackettLP,IsolationandCharacterizationofaPseudomonasspThatMineralizesthesTriazineHerbicideAtrazine[J]Appl.Environ.Microbiol.,1995,61(4)14511457[11],,,ND3[J],1998,17(5)434438[12]CaiB,HanY,LiuBetal,IsolationandCharacterizationofanAtrazineDegradingBacteriumfromIndustrialWastewaterinChina[J]Lett.Appl.Microbiol.,2003,36(5)272276[13],,(GB215232008)[S].:,2008[14]SilvaE,FialhoAM,SaCorreiaetal,CombinedBioaugmentationandBiostimulationtoCleanupSoilContaminatedwithHighConcentrationsofAtrazine[J]Environ.Sci.Technol.,2004,38(2)63726379BIOTREATMENTOFATRAZINECONTAININGINDUSTRIALWASTEWATERANDBIOREMEDIATIONOFATRAZINECONTAMINATEDSOILBYARTHROBACTERSPSTRAINAD30ANDPSEUDOMONASSPSTRAINAD39ZHUXikunWANGXuehanYAOJinchengLIQingyanNIUShuminCAIBaoli(TheKeyLaboratoryofBioactiveMaterials,MinistryofEducation,CollegeofLifeSciences,NankaiUniversity,Tianjin,300071,China)ABSTRACTArthrobacterspstrainAD30andPseudomonasspstrainAD39withhighatrazinedegradingactivityanddifferentdegradativecharacteristicswereusedforthebiotreatmentofatrazinecontainingindustrialwastewaterandbioremediationofatrazinecontaminatedsoilBioreactorexperimentusingpolyurethanefoamsasacarriershowedthatCODCroftheeffluentwaslessthan100mgl-1andconcentrationofremainingatraz