Ca2强化短程硝化颗粒污泥培养吕永涛

99环境工程学报Vol．9No．920159ChineseJournalofEnvironmentalEngineeringSep．2015Ca2+*710055SBRDO＞2mg/LCa2+。110mg/L300mg/L30d80%～85%。50mg/LCaCl2Zeta－21.4mV－13.6mV26.82mg/g51.99mg/g90d300μm68%。ZetaCa2+Ca2+。Ca2+ZetaX703.1A1673-9108201509-4112-05StrengtheningcultivationofnitritationgranularsludgebyaddingCa2+LüYongtaoJiaYanniJuKaiZhaoJieMiaoRuiWangLeiSchoolofEnvironmentalandMunicipalEngineeringXi’anUniversityofArchitectureandTechnologyXi’an710055ChinaAbstractThecultivationofnitritationgranularsludgebyaddingCa2+wasinvestigatedinasequencingbatchreactorSBRunderaerobicconditionsDO＞2mg/Lbyseedingconventionalactivatedsludge．Theni-triteaccumulationineffluentreached80%～85%byincreasinginfluentammoniaconcentrationfrom110to300mgN/Lafter30daysindicatingtherealizationofnitritation．TheZetapotentialofthesludgesurfacein-creasedfrom－21.4mVto－13.6mVandtheamountofextracellularproteinsincreasedfrom26.82mg/gto51.99mg/gbyadding50mg/LCaCl2．Granuleswithdiameterslargerthan300μmaccountedforaround68%after90days’continuousoperation．OnonehandtheelectrostaticrepulsionamongtheflocswerereducedwiththeincrementoftheZetapotentialontheotherhandtheincreasedextracellularproteinscanbecombinedtoformbiologicalpolymersunderthebridgingeffectofCa2+．Thecollaborationofbothacceleratedtheformationofnitritationgranularsludge．KeywordsnitritationgranularsludgeCa2+Zetapotentialextracellularprotein51108367201161201200092014JQ724311JS0562014－06－202014－08－041980—。[e-mail]*[e-mail]-oxidizingbacteriaAOB。、1。-、-、CANON。。。、、23CANON4。2、356。Ca2+。SBRDO＞2mg/L9Ca2+CANON。11.1SBR16LH/D=100/110.5。DO2.8～7.1mg/L。SBR6h1min320min5min1。26±2℃。1SBRFig.1SchematicdescriptionofSBR1SBRTable1OperationalconditionsinSBRdNH+4-Nmg/LminminCODmg/L11～911032030320210～2020032020320321～4630032010400447～120300320103201.2、3417mg/LSVI102mL/g。、、、50mg/LZn2SO4·7H2O0.12mg/LFeSO4·7H2O10mg/LMnCl2·4H2O0.12mg/LNiCl2·6H2O0.1mg/LAlCl3·6H2O0.05mg/LNH46Mo7O24·4H2O0.05mg/LMgSO4·7H2O20mg/LEDTA-2Na10mg/LCuSO4·5H2O0.03mg/LCoCl2·6H2O0.1mg/LH3BO30.05mg/LK2HPO410mg/LKH2PO410mg/L。。pHEPS、。1.3MLSS、NH+4-N、NO!2-N、NO!3-N、、N-1--、7pHPHM210HACHUSADOHQ25dHACHUSASEMLS230/SVM+ZetaZEN3690。1.4EPS80g/gVSS170r/min4h。13000r/min、4℃20min。0.45μmEPS。EPSEPS。LowrygVSSmgmg/g。22.1。2、。1～9d110mg/LNO－3-N80mg/L10mg/L。10～20dNH+4-N200mg/LNO－3-N160mg/L0。21300mg/L31149205mg/L。3139mg/L190mg/L83%。99%80%～85%。2、、Fig.2VariationofnitrogenconcentrationNH+4-NNO－2-NNO－3-Nininfluent/effluent2.2。3。54.97μm3a。30min10min。303b。60168.6μm3c。80215.3μm3d。3e3f。。4900～40、40～150、150～300、300～600600μm3%、15%、14%、62%6%300μm68%。SBRMLSSSVI5。MLSS。3Fig.3Variationofsludgeduringnitrifyingcultivation490Fig.4Particlesizedistributionofgranulesonday9030min10min、MLSS3417mg/L2068mg/LMLSSMLSS4905mg/L。SVI。25SVI188mL/gSVI39mL/g。33.150%41149Ca2+5SVIMLSSFig.5TimecoursesofSVIandMLSS。pH、DO、、freeammoniaFA。AOB0.50mg/LDONO－2-N89。2L/minDO2.8～7.1mg/L。DO。FAFA0.1～1.0mgN/LNOB10。FApHNO－2-N。300mg/LFA14.69mgN/LNOBFA。1126±2℃。FA。3.2。12。3。AOBEPS、。Ca2+、。Ca2+Zeta1314。6Zeta－21.4mV－13.6mV。Ca2+、、。6ZetaFig.6TimecoursesofZetapotentialCa2+EPS。713mg/g26.82mg/g51.99mg/g。Ca2+。15Ca2+EPS。7EPSFig.7VariationofpolysaccharideandproteincontentinEPS41SBRDO＞2mg/L110mg/L300mg/L30d80%～85%。250mg/LCaCl2Zeta51149－21.4mV－13.6mV26.82mg/g51.99mg/g90d300μm68%。ZetaCa2+。1．．200632114-20ZhengPingJinRencunLuGang．Kineticscharacteristicsofshortcutnitrifyingreactor．JournalofZhejiangUniversityAgriculture＆LifeSciences200632114-20inChinese2．C/N．20133382226-2233LiuWenruDingLinglingWangJianfangetal．Granula-tionofgranularnitrosationsludgeatlowC/Nratio．ActaScientiaeCircumstantiae20133382226-2233inChinese3．．201331585-90LiuGuoyangLiJunZhaoBaihangetal．Studyontherapidcultivationofshort-rangenitrifyinggranularsludgeandthenitrificationcharacteristics．EnvironmentalEngi-neering201331585-90inChinese4LvYongtaoWangLeiSunTingetal．Autotrophicnitro-genremovaldiscoveredinsuspendednitritationsystem．Chemosphere2010792180-1855SchmidtJ．E．AhringB．K．GranularsludgeformationinupflowanaerobicsludgeblanketUASBreactors．Bio-technologyandBioengineering1996493229-2466RoseR．K．Theroleofcalciuminoralstreptococcalaggre-gationandtheimplicationsforbiofilmformationandreten-tion．BiochimicaetBiophysicaActaBBA-GeneralSub-jects20001475176-827．4．20028．DOFA．20115122677-2680YuDeshuangYinJinlanWangXiaoxiaetal．StudyonstabilityofshortcutnitrificationbycontrollingDOandFA．ChineseJournalofEnvironmentalEngineering20115122677-2680inChinese9Vázquez-PadínJ．R．FigueroaM．CamposJ．L．etal．NitrifyinggranularsystemsAsuitabletechnologytoobtainstablepartialnitrificationatroomtemperature．SeparationandPurificationTechnology2010742178-18610KimD．-J．SeoD．Selectiveenrichmentandgranulationofammoniaoxidizersinasequencingbatchairliftreactor．ProcessBiochemistry20064151055-106211WinkerM．K．H．KleerebezemR．KueneJ．G．etal．Segregationofbiomassincyclicanaerobic/aerobicgranularsludgeallowstheenrichmentofanaerohicammo-niumoxidizingbacteriaatlowtemperatures．EnvironmentScience＆Technology201145177330-733712．．200727101604-1611GaoJingfengZhouJianqiangPengYongzhen．Rapidcultivationofaerobicgranularsludgeforshortcutnitrif