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,,,.60Co2[J].,2005,25(4):540-544CAODeju,PANGXiaokunZHOUShihua,etal.Studyondomesticwastewatertreatmentwithinductedactivatedsludgeby60Co2Radiation[J].ActaScientiaeCircumstantiae,2005,25(4):540-54460Co21,3,1,1,1,2,2,31.,230036;2.,230036;3.,230036:60Co,COD,DNA,DNA,ERIC2PCR.(503000Gy)60Co.,SV30SVIMLSS,,500Gy;COD,(50Gy100Gy)92195%94111%,,;60Co,DNA,PCR.3000Gy,,DNAPCR,.:60Co2;;:025322468(2005)0420540205:X70311:AStudyondomesticwastewatertreatmentwithinductedactivatedsludgeby60Co2RadiationCAODeju1,3,PANGXiaokun1,ZHOUShihua1,DUANBo1,XUMingzhao1,LUCuizhen2,WANGShangfei31.CollegeofResourcesandEnvironment,AnhuiAgricultureUniversity,Hefei2300362.CollegeofLifeScience,AnhuiAgricultureUniversity,Hefei2300363.CentreofRadiationofScienceandTechnologyofChina,Hefei230026Abstract:Theeffectof60Co2radiationontheactivatedsludgeusedfordomesticwastewatertreatmentwasinvestigated.Thechangesofactivatedcharacteristics(SV30,SVI,MLSS,increasingrateofsludge)ofthesludgesuchas,NH+42NandCODremovalcapabilitiesweremeasured,andthevariationoftotalDNAinacti2vatedsludgebeforeandafterradiationwasalsoanalyzed.TheDGGEmethodcombinedwithERIC2PCRcommunityfingerprintingwasemployedtoevaluatethechangesofbacterialcommunityinactivatedsludgeundervariousintensityofirradiation(50Gy3000Gy).TheresultsindicatedthattheSV30,SVI,MLSS,andgrowingrateofactivatedsludgeallwerelowerthanthatofthecontrol,especiallyforthegrowingrateofsludge,itdroppedsharplyoncetheirradiationintensitywasover500Gy,andCODremovalratewasalsolowerthanthatofthecontrol.ButNH+42Nremovalrateinlowerirradiationintensity(50Gy100Gy)washigherthancontrol,however,itdecreasedwhenirradiationintensitywasincreased.ThedifferenceoftotalamountsofDNA,DGGEfiguresandPCRfingerprintsintheac2tivatedsludgewereobservedafter60C2radiation,impliedthatsomevariationofthebacterialoccured,whenirradiationintensitywasbeyond3000Gy,theharmtobacterialwasgreater.Asaresult,thepolymorphismofERICthatthecommunityofthemicroorganismhadbeenchangedconstitutionally.Keywords:60Co2radiation;activatedsludge;bacterialcommunitystructure:2004207216;:2004212228:[2003]022;(2004kj148):(1965),,(),caodeju@etang.com;3(),..,,,,,[15].,25420054ActaScientiaeCircumstantiaeVol.25,No.4Apr.,2005[2].Cr()[6],.,,,.,,.[7],,,.60Co,,,,,.60Co,,COD.60Co,.1111,,8715%.:AnKe.TGL216GB,JJ23,DYY25,HeMa,HeMa,.[5],COD18514mgL-1,16125mgL-1.:TaqDNAdNTPsangon,200bpDNALadderTris,PCR:ERIC1R:5ATGTAAGCTCCTGGGGATTCAC3;ERIC2:5AAGTAAGTGACTGGGGTGAGCG3.1121121160Co,:50,100,250,500,1000,3000Gy7,(0).11212(1[5]),23h,1h,,,.3d,110210mgL-1,,5,.,.3.1)310g,1000mL,5[5],(120rmin-1).1520.2)(28,120rmin-1).11213MLSSSV30SVI[8].COD(NH+42N)[9].11214DNADNADNA[10],115%SDSDNA.018%,40V[12,14].11215ERIC2PCRPCR,25LPCR[1215],947min,941min;551min;722min;7210min;35[1215].HeMa.115%,40V,,HeMa.2,310g,5[5].,7dMLSSSV30SVI,1,3.60Co2COD,2.DNA,DNA,1.DNA,1454:60Co2.(23),3.,ERIC2PCR[13]60Co2100GyDNAPCR,4.1Fig.1Thetrendofparameterchangeoftheactivatedsludgeindifferentirradiationintensity2Fig.2NH32Nremovaleffectiveoftheactivedsludgeindif2ferentirradiationintensity1DNATable1TotalamountofDNAoftheactivatedsludgeindifferentirradiationintensityPGyDNAP(gmL-1)123024.823.425.6505.47.519.41009.424.218.825015.421.427.050016.22327.4100014.021.821.230006.48.24.71:DNA;2:DNA;3:DNA3DNA117ck;2.50Gy;3,8:100Gy;4,9:250Gy;5,10:500Gy;6,11:1000Gy;12.3000GyFig.3TheDGGEfigureoftotalDNAoftheactivatedsludgeindifferentirradiationintensity4DNAPCR(1.DNALadder(210kb,118kb,116kb,114kb,112kb,110kb,018kb,016kb);2.ck;3.100Gy60Co2)Fig.4PCRfingerprintsofactivatedsludgeindifferentirradiationintensity31,60Co2(MLSSSV30SVI),,3,503000Gy..,50Gy,24525500Gy,,500Gy60Co2.,60Co2,,,.COD9018%9412%,9511%,.3000Gy,COD9018%.,2,(50Gy100Gy250Gy)92195%89110%,.,,1000Gy,53159%61163%,.,,,,(50Gy100Gy250Gy).,,,.,,,,.1,1DNA23,,.,,.3DNA,12,3000GyDNADNA,3000Gy,,.350GyDNA,,DNA,DNA,.,MLSS,SV.,(01934165Gy)60Co2[3,8],60Co250Gy,,,;,50Gy60Co2,.3,DNA,.,50Gy,DNA.,DNA,DNA,.,.1000Gy,DNA,DNA.,,.DNA,3000Gy,DNA,,,.4,100GyDNA,,,DNAPCR,.,,.,.503000Gy60Co2,,.,,,.[2],,,.41)60Co2503000Gy,MLSSSV30SVI,,500Gy,.2)60CoCOD90189412%,,53159%94111%,50Gy100Gy92195%94111%,.3)60Co3454:60Co2,DNADNAPCR:503000Gy,,,.(References):[1]QuanBC.Bio2enhanceandutilizeinwastewatertreatment[J].En2vironmentalSciresearch,1999.12(3):2226(inChinese)[2]YuZL.IonBeamBiotechnologyForeword[M].HeFei:AnHuisci2encetechnologypress,1998:241249(inChinese)[3]ZhouQY,GaoTY.EnvironmentEngineeringprojectmicrobiology[M].Pekin:HigherEducationPress,2000:134,231(inChinese)[4]XuYT,HuangMS.Wastewatertreatmentrunmanageandabnor2malmeasure[M].Beijing:Chemistryindustrypress,2002:130153(inChinese)[5]ChengSP.ALaboratoryManualOfEnvironmentalBiotechnology[M].Nanjing:NanjinguniversityPress,1995:107109(inChi2nese)[6]ChenL,QiuTS,ChenM.TestresearchofbacteriumvariationbyUVirradiationtoremovalCr()inwater[J].Knitindustry,2004,32(2):114116(inChinese)[7]JiangYX,SunPS,LiuAW,etal.enhanceofactivatedsludgere2movalphosphorusbyUVirradiation[J].ChinEnvironSci,2003,23(2):184188(inChinese)[8]ZhangZJ.Engineeringprojectofdrainoffwater[M].Beijing:Chinconstructindustrypress,1996:162166(inChines