CuONPs对好氧颗粒污泥形成的影响研究周橄

47820188AppliedChemicalIndustryVol．47No．8Aug．20182018-03-212018-05-0251678214“”2016YFC0400804BK20161505PAPD1992－。[e-mail]1976－。CuONPs2100984SBＲCuONPs。CuONPs00．51．0mg/L2．0mg/L26242827d。0．5mg/LCuONPs。CODTNCuONPsTPCuONPs。CuONPs00．51．0mg/L2．0mg/LTP71．21%70．34%69．33%60．84%。4OUT163715621958158416．5%15．75%19．74%15．97%CuONPs。CuONPs2mg/Lββ-ProteobacteriaSBＲ4CuONPsβ。CuONPsTQ016X703A1671－3206201808－1581－06EffectofCuONPsonthecultivationofaerobicgranularsludgeinSBＲsZHOUGanZHENGXiao-yingZHOUXiangJINMeng-qiLUDanZHANGYuanSHAOXiao-yaoKeyLaboratoryofIntegratedＲegulationandＲesourcesDevelopmentonShallowLakesMinistryofEducationCollegeofEnvironmentalHohaiUniversityNanjing210098ChinaAbstractTheeffectsofCuONPsonthegranulationofaerobicsludgewereinvestigatedinfourSBＲsfed．Theresultsdemonstratedthatactivatedsludgecanbecultivatedasaerobicgranularsludgeandgranularsludgebegantoappearonthe262428dand27dat00．51．02．0mg/LCuONPsrespectively．0．5mg/LCuONPscouldstimulatemicrobialgrowthandimprovethesludgesettlingproperty．DuringtheexperimenttheremovalefficiencyofCODandTNwaslessaffectedbyCuONPswhiletheremovaleffi-ciencyofTPwasgreatlyinfluencedbyCuONPs．AsCuONPsincreasedfrom0to2mg/LtheremovalefficienciesofTPinthefourreactorswere71．21%70．34%69．33%and60．84%respectively．HighthroughputsequencingfoundthatthenumberofspecificOUTof4systemswere163715621958and1584respectivelyaccountingfor16．5%15．75%19．74%and15．97%ofthetotalsampleswhichindicatingthattheadditionofdifferentconcentrationsofCuONPsintheprocessofaerobicsludgegranu-lationhasagreatinfluenceonthedistributionofmicrobialcommunityingranularsludge．Analysisofsludgemicrobialcommunitystructureillustratedthattheproportionofβ-Proteobacteriawhichcontainedmostphosphorus-accumulatingbacteriadecreasedsignificantlyin2．0mg/LCuONPs．ThemainreasonforthedeteriorationofthephosphorusremovalperformanceofSBＲ4intheprocessofsludgegranulationcamefromtheinhibitionofmicrobialgrowthinβ-ProteobacteriabyhighconcentrationsofCuONPs．KeywordssequencebatchreactorCuONPsaerobicgranularsludgeculitvationhighthroughputse-quencingDOI:10.16581/j.cnki.issn1671-3206.2018.08.00447CuONPs、、1。CuONPs、。、、2-3。CuONPs1mg/L4。CuONPs、。11．1、、、＜50nmTEM29m2/gMLSS4000±120mg/L。B5A124SDS-8510DTNIKONYS100202-2ASSP-752PC5B-1COD。1．21．2．11。、、NH4ClKH2PO41mg/LCaCl270MgSO428Fe-SO4283mLFeCl3·6H2O1．50MnCl2·4H2O0．12CoNO32·6H2O0．18ZnSO4·7H2O0．12KI0．03CuSO4·5H2O0．03Na2MoO4·2H2O0．06H3BO30．15。1Table1Compsitionofsyntheticwastewater/mg·L－1123350250150350250150NH4Cl266152152KH2PO46544201．2．2CuONPs5mgCuONPs1L0．1mmol/LSDBSlh25℃120W40kHz5mg/LCuONPs。1．3SBＲ4SBＲ7LΦ×H=120mm×700mm1。6h、、、、。CuONPs。2/3。15～22℃pH6．5～7．5。COD、TN、TP、MLSSSVI5。1SBＲFig．1TheSBＲexperimentaldevice1．2．3．4．5．6．7．8．9．10．1．430min。DNAEzupDNA16SrＲNAV3、V4341FCCCTACACGACGCTCTTCCGATCTGCCTACGGGNGGCWGC-AG805ＲGACTGGAGTTCCTTGGCAC-CCGAGAATTCCAGACTACHVGGGTATCTAATCCPCＲ。PCＲ95℃3min94℃20s、55℃20s、72℃30s572℃5min10℃。MiSeq。22．128518CuONPsSBＲ1、SBＲ2、SBＲ3、SBＲ4CuONPs00．51．02．0mg/L。6dSBＲ1SBＲ2SBＲ2SBＲ3。18dSBＲ1SBＲ226dSBＲ1SBＲ12SBＲ2SBＲ1SBＲ3SBＲ4。a．b．6dc．18dd．26d2SBＲ1Fig．2ConfigurationofgranularsludgeinSBＲ1CuONPs2。2Table2FormationtimeofaerobicgranularsludgeCuONPs/mg·L－1/d/d026390．524521．028672．027752CuONPsSBＲ1～SBＲ439526775d1．02．0mg/LCuONPs。CuONPsCuONPs。2．2MLSS、SVIMLSSSVI3。3MLSSSVIFig．3ChangesofMLSSandSVIinprocessofaerobicgranulation34100mg/LMLSSSVI。30dSBＲ1～SBＲ4MLSS4267436842544219mg/L。SVI70dSBＲ1～SBＲ4SVI38．1339．4542．5143．22mg/g。30．5mg/LCuONPsMLSSMLSS6-7。0．5mg/LCuONPsCu2+MLSS。SVI。CuONPs0～2mg/L。2．33851474。4Fig．4Ｒemovalratesofcontaminantsinprocessofaerobicgranulationa．CODb．TNc．TPSBＲ1～SBＲ4COD82．31%81．14%82．61%81．64%96．52%95．25%95．23%95．45%。20～25dCODMLSS。CODCuONPsCOD。CuONPsTNTN78．35%79．52%77．90%79．52%90．56%89．45%89．34%88．97%。SBＲ1、SBＲ3SBＲ45～10dTN。4c1TP57．49±0．10%58．26±0．70%55．79±0．99%54．87±0．64%TPCuONPsSBＲ1～SBＲ4TP。2TPCODTP2mg/LCuONPsTP59．11±1．08%CuONPs。3SBＲ1、SBＲ2、SBＲ3TP71．21%70．34%69．33%SBＲ460．84%CuONPsCuONPs1mg/LCuONPs。2．44188688170201SBＲ1、SBＲ2、SBＲ3SBＲ439888322664718450863。5a。5b4OUT404OUT4．07%4OUT163715621958158416．5%15．75%19．74%15．97%。4CuONPs。a48518CuONPsb5Fig．5ＲarefactioncurvesandVenndiagramsofthebacterialcommunitiesα3。3αTable3Theα-diversityindicesofbacterialcommunityOTUsShannonAceChao1CoverageSimpsonSBＲ13988824354．4330395．7913790．710．960．05SBＲ23226623004．5828429．7114238．450．950．04SBＲ34718428174．7635642．6916144．450．960．03SBＲ45086323684．8023105．1711122．380．970．023AceSBＲ3＞SBＲ1＞SBＲ2＞SBＲ4Chao1SBＲ3＞SBＲ2＞SBＲ1＞SBＲ41mg/LCuONPs。CuONPsCuONPsCuONPs2．0mg/LCuOCu2+ＲOS8。2．5SBＲ1、SBＲ2、SBＲ3SBＲ46a。6a4251%6Proteobacteria、Bacteroidetes、Nitrospira、Planctomyce-tes、AcidobacteriaVerru-comicrobia。90%4。4。SBＲ1、SBＲ2、SBＲ3SBＲ475．13%78．41%75．66%61．54%SBＲ432．0mg/LCuONPs。、9SBＲ4CuONPsCu2+ＲOS。SBＲ1、SBＲ2、SBＲ3SBＲ412．88%9．68%11．45%14．75%410-11CuONPsCOD。12N2。SBＲ412．63%2mg/LCuONPsSBＲ4CODTN。CuONPs。6b4αα-Proteobacteria、ββ-Proteobacteria、γγ-Protrobacteriaδδ-Proteobacteriaαβ。SBＲ1、SBＲ2、SBＲ3SBＲ4β48．69%46．49%44．65%29．76%SBＲ4ββαγ13。β14-15。SBＲ4CuONPsβ。585147ab6Fig．6Ｒelativeabundanceatphylumandclasslevel31CuONPs00．512mg/L395267d75dCuONPsCuONPs。0．5mg/LCuONPs。CuONPs0～2mg/L。2CODTNCuONPsT