DGGE技术及其在堆肥微生物群落研究中的应用王芳

()(2008)ºOGGESSCP»TLP,3.2,.TRFLPPCR,,,,,PcR,,DNA,,,,T,TRF,,500bP,,,T-RFLP,,,RfLP,()DGGE2,2.,2,3,2,2(1.,625014;2.,625014:3,625014):DGGE,OGGE,,*,j.:OGGE;;APPlieationofDenaturingGradientGelEleetrOPhoresisonStudyofMierobefrr0CCSSLizhiong,,wengYangZleUniversity,Ya,an,Siehuan62,014,ina:lofAnimalSccc,SichuanAeultuUniversity,Ya,an.Sichuan625014,China)Abstract:DenaturingGientGelEleetrOPho51515awidesPreadmethodofsldyingMicrobialcommunitiesstructUre.APPlicationofDenaturingGradientGelElectroPhoresisonstodyofehangesofmicrobialeommunitiesineompostingProcess,wouldcontributetodeePunderstslndingofmechanismofcomPosting,dsuPPlywiththeoryguidaneeforsievingandinoculatingmierooganisms.ywords:DGGE:eomposting:microbialdiversity,,,.,,,.,,,.1)GGDGGE()rRNA/rDNARFLP()RAOP()sscP().PCR16srRNA,(OGGE)PCR,,()(2008)165rDNA,DGGEDNA(soobp),,,,)OGE,FISHDGGE,DGGE4DGGE,,,DGGE,,,,DGGE,,()(),.,,8.J.11.1,50%,()95%,()94%,()95.6%:1(%)(%)5.362.221.854.102.321.689.372.372.081942.211.342.363.040.562.3.1.2,28KD(l)28KD,(2)..___.,~l