DGGE技术在废水生物脱氮系统中的应用研究进展柳栋升

DGGE1221231．1000832．1000123．730030DenaturingGradientGelElectrophoresisDGGEDGGEDGGE。DGGEX703．1A0517－6611201119－11695－03ProgressoftheDGGEApplicationintheWastewaterBiologicalNitrogenRemovalSystemLIUDong-shengetalSchoolofCivilandEnvironmentEngineeringUniversityofScienceandTechnologyBeijingBeijing100083AbstractThebasictheoryandcharacteristicsofDGGEwereintroduced．ConsideringrecentneweststudyresultinChinaandabroadthestudyadvancesofmicrobialpopulationsinnitrogenremovalandapplicationofDGGEinwastewaterbiologicalnitrogenremovalsystemweresummarized．AtlastthecombinationofDGGEandothermoleculartechniqueswerediscussed．KeywordsDGGEMicrobialnitrogenremovalBiologicalnitrogenremovalWastewatertreatment507081208632004AA649320。[e-mail]。2011-03-31。。DGGE、16SrDNA。DGGE。1DGGEDGGEDNADNADNA。1DNA。1993Muyzer1DGGEDGGE。DGGE①②16SrDNA18SrDNAPCR③DGGE④⑤。DGGE、。22．1AmmoniaOxidi-zingBacteriaAOBNtriteOxidizingBacteri-aNOB。AOBNitro-somonas、Notrosococcus、Notrososopira、Notrosovibrio、NotrosolobusNOBNotrobact-er、Notrospira、Notrospina、Notrococcus。2．2。Pseudomonasfluorescens、Al-caligenesfaecalis、Pseudomonasaureofacie-na、Pseudomonasaeruginos。2．3NO2－-NNO3－-N。Proteus、Pseudo-monas、Micrococcus、Bacillus、Achromobacter、Aerobacter、Al-caligenes2。2．4。2080Robertson3Thiosphaerapantotropha、Pseudmonassp．Alcaligenesfaecali。4SBR3－WYLW1-6－N2O。2．5。T．panto-tropha、ThiobacilladenitficansThiomicrospiradenitrificasAlcaligeneseutrophus。5－β-proteobacteria78．33%ε-pro-teobacteria21．67%JournalofAnhuiAgri．Sci．2011391911695－11697DOI:10.13989/j.cnki.0517-6611.2011.19.066β-proteobacteria81．97%、ε-proteobacteria16．39%γ-proteobacteria1．64%。2．6AnaerobicAmmoniumOx-idationANAMMOX。1999Strous6PlanctomycetalesCandidatus“Brocadiaanammoxi-dans”。7－9CandidatusBro-cadia、CandidatusKuenenia、CandidatusScalindua、CandidatusJettenia、CandidatusAnammoxoglobus。3DGGE3．1DGGELIU10PCR-DGGE22Unculturedbacteriumβ-proteobacteria．。11DGGEA2/OAOB25℃NH4+-N95%Nitrobacter20℃NH4+-N95%Nitrospina15～20℃NH4+-N75．5%～95．0%。Ren12DGGEA/OFirmicutes、Proteobacteria、ChloroflexiBacteroidetesProteobacteria、Bacte-roidetes、Firmicutes、VerrucomicrobiaeNitrospira。3．2DGGE3．2．1SHARON。1997Hellinga13DelftSingleReactorHighActivityAmmoniumRemovalOverNitriteSHARONNOBAOB。Qiao14DGGENitrosomonas。15DGGESHARONNitrospira、NitrosospiraNitro-somonasKueneniastuttgartiensisNitrosospiraNitrosospiraNitrosomonas。3．2．2ANAMMOX。Park16DGGEANAMMOXKueneniastuttgartiensisCandidatus“Brocadiafulgida”CandidatusBrocadiasp．。Yang17DGGE。5．2kgN/m3·d11．8kgN/m3·d36．8mg/L21．5mg/LKSU-1AB057453KU2AB054007CandidatusKueneniastuttgartiensis100%。18DGGEANAMMOXANAMMOX101DGGE2Planctomycetes97%。3．2．3OLAND。OxygenLim-itedAutotrophicNitrificationDenitrificationOLAND0．1～0．3mg/LNH4+NO2+NH4+。Zhang19DGGEOLANDAOBDOAOBNitro-somonasAOB72．5±0．8%。20DGGEOLANDNitrosomonassp．Nitrosospi-rasp．Nitrococcussp．。3．2．4CANON。CompletelyAutotrophicNitrogenRemovalOverNitriteInOneSingleReac-torCANON。Zheng21DGGE110Nitrosomonassp．99%PlanctomyceteKSU-1genefor16SribosomalRNAAB057453UnculturedPlanctomycetalesbacteriumcloneR12DQ78674697%。Cho22DGGE2INH4+-N、II。2Nitrosomonassp．CandidatusBrocardiaanammoxidans。IAOBNitrosomonassp．Ni-trosospirasp．IINitrosomonassp．Nitrosospirasp．696112011Nitrosomonassp．。3．2．5SND。Simulta-neousNitrificationandDenitrificationSND。23DGGEC/NC/NNitro-somonaseuropaeaNitrobactersp．Pseud-omonassp．、Acidovoraxsp．Comamonas。Xia24DGGEC/N10∶15∶13∶1unculturedβ-pro-teobacteriaC/N10∶1ComamonasC/N5∶1NitrosomonasComamonasC/N3∶1NitrosomonasCo-mamonasMethyl-ophilus。4、、。、DGGE、DGGE。1MUYZERGWAALECUITERLINDENAG．Profilingofcomplexmicro-bialpopulationsbydenaturinggradientgelelecnoplhoersisanalysisofpol-ymerasechainreaction-amplifiedgenescodingfor16SrRNAJ．AppliedandEnvironmentalMicrobiology1993593695－700．2．M．2002378－379．3ROBERTSONLAKUENENJG．Aerbicdenitrificationacontroversyre-viredJ．ArchMicrobiol19841395351－354．4．N2O－J．2010234515－520．5．/J．2010302327－339．6STROUSMKUENENJGJETTENMSM．KeyphysiologyofanaerobicammoniumoxidationJ．AppliedandEevironmentalMicrobiology19996573248－3250．7．J．200726111867－1872．8SCHMIDMCMAASBDAPENAAeta1．Biomarkersforinsitudetec-tionofanaerobicAmmonium-oxidizinganammoxbacteriaJ．AppliedandEnvironmentalMicrobiology20057l41677－1684．9KARTALABRATTRAYJVANNIFTRIKALAetal．Candidatus“An-ammoxoglobuspropionicus”anewpropionateoxidiz-ingspeciesofanaero-bicammoniumoxidizingbacteriaJ．SystematicandAppliedMicrobiolo-gy200730139－49．10LIUXCZHANGYYANGMetal．AnalysisofbacterialcommunitystructuresintwosewagetreatmentplantswithdifferentsludgepropertiesandtreatmentperformancebynestedPCR-DGGEmethodJ．JournalofEnvironmentalSciences200741960－66．11．PCR-DGGEA2/OJ．20099553－62．12RENLJWUYNRENNQetal．MicrobialcommunitystructureinanintegratedA/Oreactortreatingdilutedlivestockwastewaterduringstart-upperiodJ．JournalofEnvironmentalSciences2010225656－662．13HELLINGACSCHELLENAJMULDERJWetal．TheSHARONProcessaninnorativemethodfornitrogenremovalfromandammonium-richwasterwaterJ．WaterSciTechnol1998379135－142．14SENQIAORYUICHIKANDATKASHINISHIYAMAetal．Partialnitri-ficationtreatmentforhighammoniumwastewaterfrommagnesiumammo-niumphosphateprocessof