DO在厌氧序批式生物膜反应器中对厌氧氨氧化反应启动的影响李祥

:　,,1984,,。＊(No.ZD081202);(No.AO030702)。DO在厌氧序批式生物膜反应器中对厌氧氨氧化反应启动的影响＊李　祥1　黄　勇1　袁　怡1,2(1.,　215011;2.,200092)　　　DO(ASBBR)。,DOASBBR,ASBBR,13d,NO-3-N、NO-2-N、NH+4-N0.25∶1.30∶1.00,100d1.560kg/(m3·d);DOASBBR,57,ASBBR,73,NO-3-N、NO-2-N、NH+4-N0.21∶1.20∶1.00,93～100d,,1.090kg/(m3·d);DO,;,(NH+4-N、NO-2-N)。　　　DO　　　Effectofdissolvedoxygenonthestart-upofANAMMOXintheASBBR　LiXiang1,HuangYong1,YuanYi1,2.(1.SchoolofEnvironmentalScienceandEngineering,SuzhouUniversityofScienceandTechnology,SuzhouJiangsu215011;2.CollegeofEnvironmentalScienceandEngineering,TongjiUniversity,Shanghai200092)Abstract:　TheeffectofinfluentDOonthestart-upofanaerobicammoniumoxidation(ANAMMOX)inASBBRwasinvestigated.ThetestwaterwithDOremovedbynitrogenstrippingwasinjectedinASBBR1,ANAMMOXbacteriawereac-cumulatedrapidlyinthereactor,andobviousANAMMOXwasobservedafter13daysofstart-upprocess.After100daysofANAMMOXprocess,theremovalrateofthevolumetricloadingoftotalnitrogenwasupto1.560kg/(m3·d).ThetestedwaterwithoutDOremovalwasinjectedinASBBR2,theremovalofNO-2-Nwasfoundstabilizedat99%duringthe57daysofstart-upprocess,whiletheNH+4-Nremovalwasverylow.WiththegrowthofANAMMOXbacteria,theNH+4-Nre-movalrateincreasedevidently,andtheremovalrateofthevolumetricloadingoftotalnitrogenwasupto1.090kg/(m3·d)during93-100days.TheexistenceDOintestwaterlaggedthestart-upofANAMMOXinASBBR,andthebiofilmenhancedtheresistancetoDOforANAMMOXbacteria.Keywords:　DO;start-up;anaerobicammoniumoxidation(ANAMMOX);anarobicsequentialbiofilmbioreac-tor(ASBBR)　　,[1,2]。,,,、pH、、(NH+4-N、NO-2-N)[3],DO。STROUS[4]595DO,,DO,DO2μmol/L。,DO,,DO,。DO,。,2(ASBBR),DO,·43·　　DODOI:10.15985/j.cnki.1001-3865.2009.10.011。1　1.1　实验装置及运行条件:2ASBBR((ASBR)),1,ASBBR1ASBBR2。ASBBR2.1L;,;9.5cm、18.5cm,。:(35±1)℃,pH(0.1mol/LHCl)7.5±0.2,ASBBR,,。1　Fig.1　SchematicdiagramofANAMMOXprocess1.2　接种污泥及预处理ASBBR1(UASB);ASBBR2。,,。1.3　进水水质,NO-2-N、NH+4-N1.3∶1.0。:NH4Cl()、NaNO2()、NaHCO30.5g/L、KHCO30.5g/L、KH2PO427.0mg/L、CaCl2·2H2O136mg/L、MgSO4·7H2O20mg/L、Ⅰ1.00mL/L、Ⅱ1.25mL/L。Ⅰ:(EDTA)5000mg/L、FeSO45000mg/L。Ⅱ:EDTA5000mg/L、ZnSO4·7H2O430mg/L、CoCl2·6H2O240mg/L、MnCl2·4H2O990mg/L、CuSO4·5H2O250mg/L、NaMoO4·2H2O220mg/L、NiCl2·6H2O190mg/L、NaSeO4·10H2O210mg/L、H3BO414mg/L。1.4　实验方法DOASBBR,ASBBR120min,DO(0.2±0.1)mg/L;ASBBR2,DO,DO(7.5±0.5)mg/L。NO-2-N,NO-2-N70.0mg/L[5],。1.5　测定方法NH+4-N;NO-2-NN-(1)-;NO-3-N;pHPH211[6]。2　2.1　ASBBR1中NH+4-N、NO-2-N浓度及总氮容积去除负荷变化ASBBR1NH+4-N、NO-2-N2,(HRT)3。ASBBR177,NH+4-N、NO-2-N50.0、65.0mg/L,(ASBBR)1∶1。,HRTASBBR1,HRT3.00d0.25d。23,NH+4-N、·44·　　31　10　200910NO-2-N,13～77d;0.050kg/(m3·d)0.460kg/(m3·d)。,HRT,NH+4-N、NO-2-N,。,。　　ASBBR178～81d,NH+4-N、NO-2-N65.0、85.0mg/L,。2,NH+4-N、NO-2-N1.0mg/L,ASBBR1。ASBBR1,NO-2-N,ASBBR182～100d,1∶3,NH+4-N85.0mg/L170.0mg/L,NO-2-N105.0mg/L221.0mg/L。23,NH+4-N、NO-2-N3.0mg/L,0.760kg/(m3·d)1.560kg/(m3·d)。2.2　ASBBR2中NH+4-N、NO-2-N浓度及总氮容积去除负荷变化ASBBR2NH+4-N、NO-2-N4,HRT5。ASBBR2NH+4-N、NO-2-N50.0、65.0mg/L,1∶1,HRT3.00d。4,ASBBR2(1～24d),NH+4-N,NH+4-N50%90%,NO-2-N1.1mg/L,NO-2-N98%。,NH+4-N4　ASBBR2NH+4-N、NO-2-NFig.4　VariationofNH+4-N、NO-2-NconcentrationinASBBR2,DO。ASBBR225～47d,NH+4-NNO-2-N,HRT3.00d2.00d。5,ASBBR20.038kg/(m3·d)0.058kg/(m3·d),。5　ASBBR2HRTFig.5　VariationofTNremovalrateandHRTinASBBR2　　ASBBR248～61d,1∶2,NH+4-N、NO-2-N100.0、130.0mg/L,HRT2.00d。4,,NH+4-N(NH+4-N35.0mg/L),,13d,NH+4-N1.0mg/L;NO-2-N1.0mg/L,NO-2-N99%。ASBBR262～67d,NH+4-N、NO-2-N150.0、195.0mg/L。4,NH+4-N、NO-2-N。ASBBR268～81d,HRT1.00d,0.50d,。45,NH+4-N,NH+4-N,NO-2-N,NO-2-N;0.320kg/(m3·d)0.680kg/(m3·d)。NO-2-N,ASBBR282～85d,NH+4-N160.0mg/L,NO-2-N(195.0mg/L)。4,NO-2-N。ASBBR293～100d,HRT0.34d。45,NH+4-N、NO-2-N,,2;,1.090kg/(m3·d)。·45·　　DO2.3　厌氧氨氧化反应启动过程比较[4]593,:(1)NO-3-N、NO-2-N、NH+4-N0.26∶1.32∶1.00,;(2)H+,,pHpH。2,DOASBBR1,NH+4-N、NO-3-N,13～77d。ASBBR1NO-3-N、NO-2-N、NH+4-NpH6。6,ASBBR113d,NO-3-N、NO-2-N、NH+4-N0.25∶1.30∶1.00,ASBBR1,;pH8.0～9.0。6　ASBBR1NO-3-N、NO-2-N、NH+4-NpHFig.6　TheratioofgeneratedNO-3-N,consumedNO-2-NtoconsumedNH+4-NandeffluentpHinASBBR1　　ASBBR2NO-3-N、NO-2-N、NH+4-NpH7。47,ASBBR2(1～24d),NH+4-N,NO-2-N,NO-2-N、NH+4-N;NO-3-N、NH+4-N0.26∶1.00,NO-3-N,NO-3-NNO-2-NDO。ASBBR2,,DO;,DONO-2-NNO-3-N,NO-2-N;,。DO,ASBBR2,ASBBR224d,NO-2-N、NH+4-N1.32∶1.00,NO-3-N,NO-3-N、NH+4-N。ASBBR257,NO-3-N、NH+4-N0.26∶1.00,ASBBR2。ASBBR273,NO-3-N、NO-2-N、NH+4-N0.21∶1.20∶1.00,ASBBR2DONH+4-NNO-2-N,(1)(3)[7]。ASBBR293,HRT,NO-2-N,NO-2-N,NO-3-N、NO-2-N、NH+4-N。7　ASBBR2NO-3-N、NO-2-N、NH+4-NpHFig.7　TheratioofgeneratedNO-3-N,consumedNO-2-NtoconsumedNH+4-NandeffluentpHinASBBR2NH3+O2+2H++2e-NH2OH+H2O(1)NH2OH+H2ONO-2+5H++4e-(2)O2+4H++4e-2H2O(3)2.4　厌氧氨氧化降解过程对比DOASBBR,ASBBR2DODO,1∶1,NH+4-N、NO-2-N50.0、65.0mg/L,NH+4-N、NO-2-N,NH+4-N、NO-2-N8。NH+4-N、NO-2-N1。8,DOASBBR2,,NH+4-N、NO-2-N20min7.5、10.0mg/L,15%;120min,NH+4-N、NO-2-N·46·　　31　10　2009101.0mg/L,99%;DO,20minNH+4-N、NO-2-N1.6、2.0mg/L,3%;20minNH+4-N、NO-2-N,ASBBR2DO,20minNH+4-N、NO-2-N;120min,NH+4-N、NO-2-N3.5、0.5mg/L,93%99%;DO,ASBBR。8　NH+4-N、NO-2-NFig.8　DegradationregulationofsubstrateinASBBRreactor1　NH+4-N、NO-2-NTable1　Matrixdegradationrate/minNH+4-N/(kg·m-3·d-1)DODONO-2-N/(kg·m-3·d-1)DODO0～200.540.120.680.1420～301.310.641.870.7930～450.831.201.151.6945～600.670.660.850.8860～750.470.730.520.9575～900