0(DPB),,,,2[1-2]。“”。DPB,(MLSS)。—、、m(COD)∶m(TN),m(COD)∶m(TP),pH[3-10],DPBMLSSMLSS,[11]。MLSS,DPBMLSS、,。11.1、,1。500mL,,,,,。4×103r/min2min,。、,5~6,,,1~2。DPB(,514015):(DPB)(MLSS),、,DPBMLSS、。:MLSS,、;MLSS、;COD,DPBMLSS;NO3-,DPB,MLSS。:;;:X2:A:1674-4829(2009)06-0024-04:2009-07-02:(1976-),,,,,.EffectofMLSSofDPBonDenitrifyingPhosphorusRemovalWANGChun-yingAbstract:Inordertoobtainabetterinsightintotheeffectofmixedliquorsuspendedsolids(MLSS)ofdenitrifyingphosphorusremovingbacteria(DPB)ondenitrifyingphosphorusremoval,theanaerobicandanoxicexperimentswerecarriedout,andtheeffectofMLSSofDPBonanaerobicphosphorusreleaseandanoxicphosphorusuptakewereinvestigated.TheresultsindicatedthatwhentheMLSSwerehigher,thephosphorusreleaseanduptakerate,andthedenitrificationratewerehigher;buttheMLSShadnosignificanteffectontherateperunitofDPBmass.Iftimewasadequate,thetotalphosphorusreleasedunderanaerobicenvironmentweredeterminedbytheavailableCODinthewastewater,andtheMLSSonlyaffectedonthearrivaltimeatthetotalphosphorusreleased.Whenthenitrogeninthewastewaterwasnotenough,thephosphorusreleasedwasmorethanthatwasre-uptaken,andwhentheMLSSwerehigher,theconcentrationofphosphorusinthewastewatertreatedwashigher.Keywords:Denitrifyingphosphorusremovingbacteria(DPB);Mixedliquorsuspendedsolids(MLSS);Denitrifyingphosphorusremoval226200912EnvironmentalScienceandTechnologyVol.22No.6Dec.20092261、、(KNO3)。1.2COD:;(NO3--N):;(PO43--P):;MLSS:。1.31.3.1,KNO3。1.3.2DPBA2N(Anaerobic/Anoxic/Nitrification)(A2N)。6、,DPB()MLSS,。22.1MLSS、A2N,4(1#,2#,3#,4#),60min,CODPO43--P;120min;KNO3,,NO3--NPO43--P。1#~4#MLSS:2000,2500,3300,5000mg/L。2.1.1MLSS2。DPB,,PO43--P,DPB。2-(a),4MLSS,MLSS。MLSS4#60min,;MLSS1#60min;2#3#。4MLSS15min1#~4#:19.80,25.32,36.68,57.72mg/(L·h),9.90,10.13,11.12,11.54mg/(g·h),MLSS,MLSS,。2.1.2MLSS,DPB,(NO3--N),NO3--NN2PO43--P,。MLSS2。2-(b),2MLSS(b)1#2#3#4#2520151050020406080100ρ(PO43--P)/(mg·L-1)t/min◆▲□(c)1#2#3#4#2520151050020406080100120ρ(PO43--P)/(mg·L-1)t/min◆▲□■1#2#3#4#25201510500204060ρ(PO43--P)/(mg·L-1)t/min◆□▲DPB(a)252009124NO3-7#PO43--P7#NO3--N8#NO3--N8#PO43--P353025201510503530252015105020406080100120140t/minρ(NO3--N)/(mg·L-1)ρ(PO43--P)/(mg·L-1)○▲◆,1#~4#(PO43-P,),MLSS4#,30min0.21mg/L,1#,2#,3#30min3.5~4.5mg/L。60min2#0.48mg/L,3#0.15mg/L,1#MLSS95min0.5mg/L。1#~4#15min:25.80,45.76,68.48,78.04mg/(L·h),12.90,18.30,20.75,15.61mg/(g·h)。MLSS;1#,2#,3#MLSS,MLSS4#2#3#,。MLSS2000~3300mg/L,MLSSDPB,,MLSS,MLSS,,MLSS5000mg/L4#。NO3-,NO3--N,2-(c),1#~4#NO-3-N,4#,30min0.32mg/L,1#,2#,3#NO3--N30min12.66,9.14,8.28mg/L。3#NO3--N60min0.16mg/L,2#90min0.51mg/L,1#,90min1.29mg/L。30min1#~4#:21.95,28.76,30.43,45.83mg/(L·h),10.97,11.50,9.22,9.17mg/(g·h),MLSS,,9.17~11.50mg/(g·h)。4#30minNO3-,PO43--P,1#,2#,3#。MLSS,NO3-,PO43--P,。2-(a)、2-(b),MLSS,,NO3-。2.2CODA2NDPBCOD,2(5#,6#),60min,PO43--P;KNO3,NO3-PO43--P。2COD85~95mg/L,:5#2800mg/L,6#4700mg/L。3。,MLSS6#,,2.1.1;60min6#7#,。,COD,MLSS,MLSS。DPBPO43--P,CODPO43--P。60minKNO3,2,MLSS6#7#,60min20.5mg/L;NO3-,6#7#。,NO3-,,NO3-(6#9min2.21mg/L,7#25min1.33mg/L)。100min2NO3-,。MLSS6#,2.1.2。2.3NO3-A2N,2(7#,8#),60min,PO43-;KNO390min,NO3-PO43-。MLSS:7#1000mg/L,8#4800mg/L。4。ρ(PO43--P)/(mg·L-1)ρ(NO3--N)/(mg·L-1)t/min16141210864201614121086420204060801001201401603COD6#NO3--N6#PO43--P○▲5#PO43--P5#NO3--N◆262264,7#,8#,MLSS8#7#60min9.81mg/L;NO3-,8#NO3-15min0,7#NO3-30min0,8#7#。KNO3,NO3-,,,8#。60min,:7#10.33mg/L,8#20.79mg/L,4.88mg/L。NO3-,DPB,,DPBMLSS。3(1)DPBMLSS,MLSS,。MLSS,NO3-,;MLSS,,NO3-。(2)、NO3-,PO43--P,、MLSS。(3),COD,DPBMLSS;DPBMLSS。(4)NO3-,DPB,,DPBMLSS。[][1]KUBAT,vanLOOSDRECHTMCM,HEIJNENJJ.PhosphorusandnitrogenremovalwithminimalCODrequirementbyintegrationofdenitrifyingdephosphatationandnitrificationinatwo-sludgesystem[J].WatRes,1996,30(7):1702-1710.[2],,.[J].,2002,3(6):65-68.[3],,,.[J].(),2007,35(6):120-126.[4],,,.[J].,2007,38(5):637-640.[5]MERZOUKIM,BERNETN,DELGENESJP,etal.BiologicaldenitrifyingphosphorusremovalinSBR:effectofaddednitrateconcentrationandsludgeretentiontime[J].WatSciTech,2001,43(3):191-194.[6],,,.[J].,2005,6(9):20-24.[7]KUBAT,vanLOOSDRECHTMCM.Biologicaldephosphatationbyactivatedsludgeunderdenitrifyingconditions:pHInfluenceandoccurrenceofdenitrifyingdephosphatationinafullscalewastewatertreatmentplant[J].WatSciTech,1999,36(12):75-82.[8]MEINHOLDJ,ARNOLDE,ISAACSS.Effectofnitriteonanoxicphosphateuptakeinbiologicalphosphorusremovalactivatedsludge[J].WatRes,1999,33(8):1871-1883.[9],,,.[J].,2003,25(6):323-325.[10],,.[J].,2008,21(5):57-60.[11],,,.、[J].,2003,19(1):33-36.()《》2008!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!DPB04030.7715230.53350%78.25050%77.25050%29.667827