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(4):218～222.[4] ,,,.[J].,2004,19(5):289～291.(:2005-10-20)(编辑 宝建喜)DTF王连方 【】 DTF。 DTF,。 0.5%～10%21.1%～97.9%,、、pH、。 DTF。 【】 ;; :R599.9 :A :1001-1889(2006)04-0205-03PreliminarystudiesofDTFemulsiononfixing-fluorideeffectforteaWANGLian-fang(XinjiangCenterforDiseaseControlandPrevention,Urumqi830002) 【Abstract】 Objective TostudytheeffectofDTFemulsiononfixingfluorideforteafluoride.Methods Observedtheeffectoffixing-fluoridebyDTFemulsionappliedonteasurface.Results Therateoffuoridefixedwas21.1%to97.9%whenaddinganamountof0.5%to10%ofDTFagentintotea.Noobviouschangeswerefoundinteacolor,polyphenolcontent,andtasteoftea-waterbythismethod.Conclusions ThereagentofDTFemulsionisaneffectivereagentforfixingfluoridefortea. 【Keywords】 Teafluoride;Fixingagent;Fixingfluoride 、、。。,350mg/kg,700mg/kg[1]。,,[2～6]。90,,。,≤300mg/kg,500～800mg/kg,40%～62.5%。。DTF[7],。DTF,,90%(10%)。 :( 830002) :(1941-),,,。1 :DTF,,。 :,。 :,,,,。 :2.0g(,2.0g),250ml(0.18mg/L),100℃3h,,、。 ;721,470nm,1cm;(GB8313-87)。2 2.1 DTF 0、0.5%、1.0%、2.0%、2.5%、5.0%、10%,·205· 2006 21 4 ChinJCtrlEndemDisVol.21No.42006 ,,(0),,,(1)。0%～10%,0%97.9%。1 (%)00.51.02.02.55.010.06333333Fmg/kg(x±s)507.7±17.0400.4±14.4327.5±6.3260.8±9.6232.9±6.294.6±6.210.8±10.6(mg/kg)0107.3180.2246.9274.8413.1496.9(%)021.135.548.654.181.497.9 :Fmg/kg(x)(y):r=-0.916,90.01(5)=0.875,p0.01,lg(y+1)=1.01747-0.0021X;X(y),r-0.916,n'=5,p0.01,lg(y+1)=1.4537+0.6852lg(X+0.01)。2.2 (y)Fmg/kglg(y+1)=1.01747-0.0021X,X300mg/kgy=1.44%,1.5%,,,300mg/kg(2)。2 1.5%66(mg/kg)514.38±15.96295.21±10.99(%)42.612.3 、、pH,,,2.5%,5%(3)。3 2.5%(%)66(mg/kg)501.47±18.29215.65±21.4957.0()0.64±0.0140.62±0.0333.12(%)10.80±0.6510.70±0.670.933 3.1 ,。、、、,、、0.4g,40%,200%～333%。。DTF,0.2g,20%。,。DTF,0.5%20%,2.5%,50%,5%80%,10%95%。DTF300mg/kg。DTF,,,,DTF。,DTF,。3.2 DTF DTF,(10mg/L),1.0mg/L16～20mg/g,。DTF,,。10mg/g。,,,,。,。,,。[8]。[1] .[J].,2004,19(2):74～77.[2] ,,,.[J].,1986,5(2):110～113.·206· 2006 21 4 ChinJCtrlEndemDisVol.21No.42006[3] ,,,.[J].,1993,8(3):43～48.[4] ,,.[C].,1996,119～123.[5] .[J].,2001,20(1-1):188～192.[6] ,,.[J].,2001,20(1-1):198～200.[7] .DTF[J].,2003,18(1):17～19.[8] ,,,.[J].,2005,12(3):35～38.(:2005-11-07)(编辑 杨凤山)Ⅰ张秀云,任立群,申华峰,李相军【】 (Se)(norepinephrineNE)(cardiacfibroblastCFbs)ⅠmRNA。 WistarCFbs,NESe(Selenium)48h,Ⅰ,RT-PCRmRNA。 NEⅠ(A)(P0.01);SeANE(P0.01);SeNE,(P0.01)。 NEⅠCFbsα1ⅠmRNA,SeⅠ。【】 ;;Ⅰ:O613.25 :A :1001-1889(2006)04-0207-03EffectofseleniumoncollagenIsynthesizingmetabolisminCardiacfibroblastsZHANGXiu-yun,RENLi-pun,SHENHua-feng,etal(InstituteofFrontierMedicalScience,Changchun130021,China)【Abstract】 Objective Toinvestigatetheeffectofselenium(Se)oncollagenIsynthesizingmetabolismandmRNAex-pressioninculturedCardiacfibroblasts(CFbs)treatedwithNorepinephrine(NE).Methods CFbswereobtainedbythemethodofenzymaticdispersionculture.Cellandculturemediumwerecollectedafter48h,thenexaminingthehydroxyproline(HYP)andcollagenIcontentaswellastheexpressionofα1[I]collagenmRNA.Results TheconcentrationofHYPinculturemediumwasobviouslyhigherinNEgroupthanthatinthecontrol(P0.01),however,theconcentrationofHYPinculturemediumsig-nificantlyreducedincellstreatedwithSe+NEcomparedwiththatintheNEgroup(P0.01);ThelevelofcollagenImRNAinculturemediumwasobviouslyhigherinNEgroupthanthatinthecontrol(P0.01),andthelevelofcollagenImRNAincul-turemediumsignificantlyreducedincellstreatedwithSe+NEcomparedwiththatintheNEgroup(P0.01).Conclusions NEcanincreasethecontentoftheHYPandTypeIcollagen,however,SecouldinhibitthesynthesisandsecretionofHYPandTypeIcollagen,Secouldalsodecreasetheexpressionofα1[I]collagenmRNA.【Keywords】 Selenium;Cardiacfibroblasts;TypeIcollagen ,,,,。,,,:( 130021):(1965-),,,,,。。(Se),Se,[1,2]。Se(NE)(CFbs)CFbs,,。·207· 2006 21 4 ChinJCtrlEndemDisVol.21No.42006