EGSB反应器在20下处理啤酒废水的工艺及微生物学研究

EGSB20,3,(,100084):20(EGSB),,:HRT18h,184d,EGSB(COD)10kgP(m3d),COD85%,1kgCOD0158m3;,,,10kgP(m3d),;,(ECP),;,20,156.:EGSB;;:X797:A:025023301(2008)0420990206:2007204224;:2007207223:(50678091):(1982),,,,E2mail:[e-mail]3,E2mail:[e-mail]lCharacteristicsofanEGSBReactorTreatingBreweryWastewaterUnder20LIJin,ZUOJian2e,XINGWei(DepartmentofEnvironmentalScienceandEngineering,TsinghuaUniversity,Beijing100084,China)Abstract:Alab2scaleEGSBreactorfedbyartificialbrewerywastewaterwasoperatedunder20continuously,anditsprocessperformance,characteristicsofgranularsludge,microbialcommunitystructurewereinvestigated.Theresultsindicatedthat:after184daysoperation,theorganicloadingrateofEGSBreactorcouldreach10kgP(m3d)atHRTofabout18h,theCODremovalefficiencieswereabove85%,andthespecificbiogasproductionwasabout0158m3perkilogramCODremoved.Astheorganicloadingrateincreased,theaveragediameterofthegranuleincreased,andthesettlingvelocityincreasedtoo,butwhentheloadingratereachedabout10kgP(m3d),thegranularaveragediameterdecreasedslightly.Comparedwiththegranularsludgeoperatedatmesophiliccondition,theECPcontentsespeciallytheproteincontentsinthegranularsludgeoperatedat20increasedobviously.Comparedwiththeseedsludge,themainArchaea(methanogenes)speciesinthesludgesamplesoperatedatdifferentloadingratesunder20didnotchangegreatly,butthedominantmethanogenesinthesludgeoperatedunder15for6monthsseededwiththesamemesophilicsludgechangedobviously.Keywords:EGSBreactor;ECPs;microbialcommunitystructure,[1].,(35)(55),[2,3].,,[47].,,[811].,EGSB620,(extracellularpolymers,ECP),,(20).11111,(expandedgranularsludgebed,EGSB),85mm,2040mm,1760mm;15L,10L,5L.,DC2101020.29420084ENVIRONMENTALSCIENCEVol.29,No.4Apr.,2008,,.,.1EGSBFig.1SchematicdiagramofEGSBreactorandprocessflowchart112,,,1200mgPL,7500mgPL,1mL(Fe=0113gPL,Co=01005gPL,Mo=0103gPL,Ni=01002gPL)012mL,,pH710.,20LPd,HRT18h,.113(35)UASB,016210mm,VSSPSS0186,2617gPL.114COD:TL21ACOD;pH:Orion828;:;:LML22;:[3];:[12];:S2570;SSVSS:[3];:1m[3];:,TP22060T,2m(3015mm),50;(ECP):EDTA,,[1316].PCR2DGGE:DNA,PCRPTC2200(MJ),16SrDNAV22V3,PARC109FPRUN518R,DGGEDcode(Bio2Rad),8%,30%55%,601012h,SYBRGold.221120EGSB,115kgP(m3d),184d,50d.EGSBPCOD2,3.20d,20.,EGSBCOD1200mgPL,COD50%70%,,,23.COD85%,.,EGSB5116kgP(m3d)21641061610kgP(m3d),2,EGSB35d,COD,35d,COD85%,616kgP(m3d)10kgP(m3d),10dCOD70%,6d,COD85%.,,COD,728096d,,,18,COD74%80%69%,;,,143172d,18,COD,,.3,EGSB(),,1994:EGSB20013LP(Ld)418LP(Ld).,,EGSB201kgCOD0158m3.75%,,,:CO2,,.2EGSB20Fig.2PerformanceofEGSBreactorat20320EGSBFig.3RemovalorganicloadingratesandspecificbiogasproductionratesofEGSBreactorat202126,,4.,114mm56%85%,2mm,30%70%.10kgP(m3d),,,114mm,2mm10,10kgP(m3d),,.2mm1142mm,;,,:,Allen[17],5.1105gPcm3,4Fig.4GranulardiameterdistributionatdifferentOLRs5Fig.5AveragegranulardensitiesatdifferentOLRs299291101103gPcm3,;10kgP(m3d).,,,,10kgP(m3d).,,,.,,,.20,,,,,.184d,,,6.,,,[6(a)];,[6(b)];[(6(c)];,1P2,,[6(d)];,7080m,,,[6(e)];[6(f)],.620184dFig.6SEMphotographsofgranulesfromEGSBreactorat20after184daysoperation213ECPECP[1316].7,EGSB.:,ECP.4106161010kgP(m3d),(VSS):111314mgPg172756mgPg;ECP;115141,ECPVSS218%710%.UASB,35,10kgP(m3d),,14mgPg,44mgPg.20EGSB,35ECP,.,ECP,.ECP,3994:EGSB20,.720ECPFig.7ECPcontentsandcompositioninthesludgeoperatedat20214[1821],[2224],[24,25].PCR2DGGE20EGSB156(),8.DGGE,,.8A15,B,CDE20.8:,,;320,,156,,:156(A)812,(B)320(CDE).,BCDE,A,A.3(1),20A:15;B:(35);C:20,1.6kg(m3d)-1;D:20,2.6kg(m3d)-1;E:20,4.0kg(m3d)-18DGGEFig.8DggefingerprintofthesamekindofsludgeunderdifferenttemperaturesEGSB,HRT18h,184d,10kgP(m3d),COD85%,1kgCOD0158m3.(2),,.10kgP(m3d),,.ECP,,ECP,,.(3),20,,156.:[1]LettingaG,RebacS,ZeemanG.Challengeofpsychrophilicanaerobicwastewatertreatment[J].TrendsinBiotechnology,2001,19(9):3632370.[2],.[M].:,2004.29.[3].[M].:,1998.5222560.[4]LettingaG,VanlierS.Advancedanaerobictreatmentinnearfuture[J].WaterScienceTechnology,1997,35(10):5212.[5].[J].,1998,19(1):94296.[6]LettingaG,RebacS,ParshinaS,etal.High2rateanaerobictreatmentofwastewateratlowtemperature[J].AppliedEnvironmentalMicrobiology,1999,65(4):169621702.[7]RebacS,RuskovaJ,GerbensS,etal.High2rateanaerobictreatmentofwastewaterunderpsychrophilicconditions[J].FermentandBiotechnol,1995,80(5):4992506.[8]NozhevnikovaAN,SimankovaMV,ParshinaSN,etal.Temperaturecharacteristicsofmethanogenicarchaeaandacetogenicbacteriaisolatedfromcoldenvironments[J].WaterScience49929Technology,2001,44(8):41248.[9]SharonMH,CartonM.Methanogenicpopulationstructureinavarietyofanaerobic[J].FEMSMicrobiologyLetters,2003,219:2972304.[10]ConnellyTL,TilburgCM,YagerPL.EvidenceforpsychrophilesoutnumberingpsychrotolerantmarinebacteriainthespringtimecoastalArctic[J].LimnologyandOceanography,2006,51(2):120521210.[11],.[J].,1999,26(2):155.[12],.[P].():20051001187418,2005211216.[13],,,.[J].,1994,15(4):23226.[14]SponzaDT.Extracellularpolymersubstancesandphysicochemicalpropertiesofflocsinsteadyandunsteadystateactivatedsludgesystems[J].ProcessBiochemistry,2002,37:9832998.[15]LiuWT,ChanOC,FangHP.Characterizationofmicrobialcommunity