搜索
25 120083 JournaloftheHebeiAcademyofSciencesVol.25No.1Mar.2008＊:1001-9383(2008)01-0052-05EGSB贾 璇1,徐建中1,王路光2,王靖飞2(1., 071000;2., 050051):采用葡萄糖自配水研究了厌氧膨胀颗粒污泥床(EGSB)反应器运行过程中颗粒污泥的形态,微生物相及优势菌群的分布状况。结果表明,随着运行时间和有机负荷的提高,反应器中颗粒污泥的粒径逐渐增大,污泥床底部颗粒污泥的粒径大于上部的污泥粒径,呈明显的分层现象。在扫描电镜下观察颗粒污泥的微生物相,运行初期甲烷丝菌属为优势菌群;随着有机负荷的提高,甲烷丝菌逐渐减少,甲烷杆菌占优势;待反应器稳定运行后,菌群类型更加丰富多样,在甲烷杆菌和甲烷丝菌上附着有部分球菌,反刍产甲烷球菌等多种产甲烷菌混栖。不同高度颗粒污泥的形态和微生物相分布也表现出特有的规律性。:EGSB反应器;厌氧颗粒污泥;微生物相;优势菌群:Q93;X172:APerformanceandmicrobialphaseofanaerobicgranularsludgeinanEGSBreactorJIAXuan1,XUJian-zhong1,WANGLu-guang2,WANGJing-fei2(1.HebeiUniversity,BaodingHebei071000,China;2.StateEnvironmentalProtectionEngineeringCenterforPharmacyWastewaterPollutionControl,HebeiProvincialKeyLaboratoryofAquaticEnvironment,ShijiazhuangHebei050051,China)Abstract:Withwatersamplepreparedbyglucoseastreatmentobject,theperformanceandmicrobialphaseofanaerobicgranularsludgeinabench-scaleEGSBreactortreatingsyntheticorganicwastewaterwerestudied.Theresultsshowedthat:alongwiththereactor′soperationandorganicloadingincreased,thediameterofgranularsludgealsoincreased,andthediameterofthegranularinthebottomofthesludgebedwaslargerthanthatintheupperpartofthesludgebed.Observedunderthescanningelectronicmicroscopy,itwasfoundedthatintheearlytime,therewasdistinctpredominanceofMethanosaeta.Alongwiththeorganicloadingin-creased,itwasappearednottheMethanosaetabuttheSarcina-typebacteria.Instableoperationatmedium,thegranuleshadmoremicrobespecies.OntheMethanosaetaandSarcina-typebacteria,thereweremanyMethanobacteriacongregateddeveloped.Theperformanceandmicrobialphaseofanaerobicgranularsludgeindifferentsiteofthereactorhadsomeregularity.Keywords:EGSBreactor;Anaerobicgranularsludge;Microbialphase;Dominantmicroflora(ExpandedGranularSludgeBed EGSB)UASB。EGSBUASB,＊:2008-01-02:;(07276708D):(1982-),,,,,.DOI:10.16191/j.cnki.hbkx.2008.01.0011 :EGSB,,,,,,。,EGSB[1-6],EGSB。EGSB,,,。1 1.1 1。EGSB,35±1℃,13.4L,2.0m。1 EGSB1.2 ,,COD∶N∶P=200∶5∶1,,pH6.8～7.5。1.3 UASB,VSS/SS0.897。1.4 CODHACH45600COD;TOCApollo9000;VFADX600;pH;;;(SS)(VSS);S-3500N。2 EGSBEGSB(1-10d)、(11-47d)、(48-59d)。,(COD2000mg/L)(4kgCOD/m3·d)。4d,,1∶1,1.0m/h。,。,。COD2000mg/L6000mg/L,4kgCOD/(m3·d)12kgCOD/(m3·d)。12kgCOD/(m3·d),,2m/h。3 3.1 EGSBEGSB,。,,1mm4mm,,。,。;,;,,、、;,,。,Ca2+。3.2 EGSB3.2.1 颗粒污泥表面和内部微生物相分布,;,53200825,,,,,,、;,,,,、、,2。,,3,,[7]。,,,。(a)(b)14d(c)54d2 (a)×200(b)×1.0k(c)×4.0k3 3.2.2 不同有机负荷下颗粒污泥的微生物相分布,。,。,4(a);EGSB4kgCOD/(m3·d),,,,4(b);10kgCOD/(m3·d),,4(c);EGSB12kgCOD/(m3·d),,,。、,4(d)。,,,EGSB。3.2.3 反应器不同高度颗粒污泥微生物相分布EGSB,。555d、。,,,;,,,;,,541 :EGSB、,,。,EGSB,。 (a)(b)4kgCOD/(m3·d)(c)10kgCOD/(m3·d)(d)12kgCOD/(m3·d) 4 (a)(b)(c)5 4 EGSB,:(1),,,,,。(2),。(3),,、,。、、,。(4),EGSB。(5)EGSB,,、。55200825:[1] ,,,.(EGSB)[J].,2001,19(2):8-11.[2] SalihRebac,JuliaRuskova,SybrenGerbens,JulesB.VanLier,AlfonsJ.M.Stams,andGatzeLettinga.High-RateAn-aerobicTreatmentofWastewaterunderPsychrophilicCondi-tions[J].JournalofFermentationandBioengineering,1995,80(5),499-506.[3] ,,,.EGSB[J].,2001,27(3):26-30.[4] Ching-ShyungHwu,JulesB.vanLierandGatzeLettinga.Physicochemicalandbiologicalperformanceofexpandedgran-ularsludgebedreactorstreatinglong-chainfattyacids[J].ProcessBiochemistry,1998,33(1):75-81.[5] ,.EGSB[J].,2006,32(2):25.[6] ,,.[J].,226,22(3):89-92.[7] ,,.[J].,2007,33(2):217-220.(上接第48页)[9] ,,.CVD[J].,2002,(3):17-19.[10] ,.[J].,2004,(2):17-18.[11] ,CVD[J].,2000,(7):39-40.[12] ,,.CVD[J].,1999,(11):8-10.[13] ,,.CVD[J].,2000,(2):27-30.[14] ,.CVD[J].,1995,(8):14-16.[15] ,,.CVD[J].,2003,(6):124-126.[16] ,,.CVD[J].,2001,(3):27-28.56