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1142330(6)[3〕Y9Fe,uaueB.I、、BieytSI,ls,”(5):”553.〔4」F19QH,zllongJJ.5iBochelm,2,37(7):769774.〔5],,,l%,,18(2):n13.[6」Ctiinc,KOvacB,iG~i~G.iBocteihlOIadniB11~irgn,【7〕,,,.,2X()1,(3):33~35.〔8」,,.,2〕l(5):45.〔9〕,2X(X),21(6):20.〔10」,.,21(4):78.〔,,.,l,,15(4):7511.【12〕,,,.,2X(X),19(4):os、3.2[3」pJP,mYM,Kimsw,`.Pnx:essBiocllellitsytr,2,37(11):〔14〕,,,.,17,16(6):14~1.6〔15〕,.(),17,37(6):54571,50(2):2072101257~1262.FISH`“(15(:、,、、。,,。,。,。:,165lrlNA,,I〕NA,:3:A:0253~2654(2X()3)011AP]ICAnONOFFLUORICENCENIlSrUElDIUITON(nSHNIICROBDEOOLOGYXNIGIngRENN-anQiWANGiJeSofMluiP,of,。15《d:Molectdartechidesaer~ivte,t,dehael,inhtestudyoncml〕ialecol.H~,meht」5doontpvideiOFmion”』tn.1`,nun」,andspialsbuonofhteCroom~.nIcont-arst,Ru~cceneshahybiridaztion()Hconineshtel〕reClisonofIonlecldaribolhthtevisui〕nlion5】~cope,dpemts~iaztionldidentconofiidnviducn〕ialcensiwd】`rnathaiatt.Hnoton」y~htedetcetionofslow〕Wgcnanls。,but〕ofyet~beeultur.ltisuseof』ofrnl”lyplnsIinidisan{198,mentofhetp。nstrucejeonx(in~6,lisawetoolform(11:ular”1.sescmbiOL.nIhit,pr,najIorteChniesan(lpSSesofFH~de-,11,」.lstapp』ic`onincn〕bialecol,aswellasporbl~,pi15,d`ltivesofH,idcsu~I·Kwon:~~ein。hy”ion(”SH),165rRN,Prolx,NPA,:ni1.dar1*973(No.CZX()26)elT:51~86282lor5182291744,E~l:ixdf@y100.~.cn:2)212,:2)3-02052〕330(6),、,。,,。,。、、。,,。。,。,(Fluo-erscecn。nIistuHybirdiaziton,HsH),,。,HSH。、、,。1FISHFISH,。:①;②;③;④;⑤;⑥;⑦,。1.1IFSH、。,。1NAFISH。30,,。。,,,。5`3`,,5`,。(、),、。1.2pNANPA(itdeNudie。Aicd),DNA,N(2),PNA。PNA,DNA。NPIANDA,,。NPA,。PNArRNA,NA,。NPA·n6·2)3os(6)rRNA,PNAHSH,DNA。NPA,,APNADNA,PNA。,,2HSHPNA。PNADNA。woens[]lFsHNPADNA5。1FISHARCgHlsEUB338[l」EUB338[2]NHGC[3〕Hga[1〕gCTCCCCCGCCAA,《1,GCCrCCCGTA;AGI,gCACCCGTAGGl,TAI,AgACG(;CCCCI,,`TTACCACC(0T165rRA165rRNA165rRA235235rrHA,915934,338355,338355,l11918,l11918Al」Plb[l〕J8〔4]CGll℃G(fC)CAGCCAG1935%8985BEIZa[l)(M42a[l〕SRB385tl](CITCCCACI,TOTTCC(1{:CCACAI,Cg1,CGC〔1℃(XGl,CAGCArehaEul〕aCtieraN〔sulfurltieraw%G+CBact%GCg-itivebacteiraa-1、。t。〕】〕actieraa~Prolaactiera,ne】、。t”1〕aclenaJ、。〕bacteira~1〕rote。】〕actiera~Pro!btiera,,gmopistivse165rRA,165rRNA,235rRA,235rRA,165rRA,10271310271《338522RsHPNA6[](5,~,3),Tm(℃)jù02OOC,7068706173nUl1BacUinl163Pse163S2tSal63S23510CTGCCrCCCGl,AG(;AACCAGACll,CCl〔:T〔:AATGAG(〕AACGI,GAAr〔:CA;A《:AO1CCTCCGTrCTAAC(AT〔;:uEcaEubadeiraJdJ.。isPeuJn““.sa5~.aueT。Z1.3。,,,。IFSH。,NHPP(23),,8。lsA(JdeisngalAlllpliifcation),(HoeradihsePoixdase,HR〕),,A1020。,,。,。IFSH。,,2)330(6)AFISH。2MulictolorFISH(MFH),,IFSH7。。eP。’keefe4NPAMlFsH、、。MFlsH,,,,,。MIFSH。(WideifeldeDconvo!u-iotnEiPfluoerscenceiMcorscoep)。(collft{alersc~igniMcorsce,CM),,CM,。3EISH,FISH,、。FISH,、。,IFSH,(irt。~ibicnsis)、(ill。)s[〕。165rRAlFsH,。,,(ozIOea)、(cAienotbacetr)〔’0〕〔”〕。islynobRert。〔`,〕lFsH,。seikguhciL`,]uAsB,,。,165rRNA,,lFsH。4FISH4.1FISHIFSH,。IFSH。,18。,rRNA,。,(),,,。·ns·2X()330(6),、。4.2FISHFISH,、、、。,FISH。,,。IFSH,,CM。、。,。,,。4.3FISH,。,。,。RNA,、RNA,,,RNADNA。。PNA,,nSH。rRNA,rRNA,,。,,。3C,。,,。5HSH,。IFSH。,(GI’eenRuooscentietn,Cl,P),DNA,。,FISH,,,。FISll,FISH。l〕】~1Rl,I」】diwgw,ieferKH.MiebiolRev,1995,59:1431692(X)330(6)119[2]aiDnslH,BnA,A~Rl,.stAppliMcorbiol,1,22:434444.L3」ROllere,WerM,11Rl,.Cnll〕101`,l4,140:28492858.[4]njAaBF,IllF,Pelerp,.1MSMieorbcEol,2l,:105113.〔5」w」Az,chihsSW,derBJ.Al1Envinllcol,2《x,“:284289.【6」l、o’k(feH,S,OliveioK,et.J.iMcd〕101Medls,21,:281292·7〕hHN,iBrl。T,F。TG,.`nce,19),:34.〔8]elFskeA,Ak~ADL,eDV、WM.Apl,nEvirnDiMcn〕bid,18,64:458845.9」R``eSUMOmRA,WrM,】R,.ApplnEviiMcbiol,l,61:70’7·〔10」werM,EdlartR,ManzW,.App}EllviorlliMciol,l4,.:792.〔11〕werM,RatllG,11`R,et`.SystemApplMICl〕101,l,18:25.[12]511」c,15G.Wat,2001,(35)11:3139.13JekSiclliy,ay,ohaSlliA,.watercSil,`〕hnol,2,45(or):1925.’(8X)ll):。,。。,,。:,,,:3:A:0253~2654)3)011905LARG-ESCALE、,EASTWO.ERll)SYbrl,IN1llEAJICAllDNONMAPOFPROEOM[EDRACTOM[EMAHdi~RJ11WeiiQunsIofJ“3,”of~,830011)Abst,d:Ptiendllignhtehtierolres,oneJmxllItWaysis、〕ughl〕mtein,1eininteradion.nIha1ionc,idelltingallofpr。iPneininteraCitoninport~dll〕pinghteprieninteraCtionsl】lathvaelnaCitng11yscientisst’auenbOll,ofchlcsaleyaesthybirdsystemi,oenslratofn】1iwdelyus.nIentwoty~,ditouspn)Jectshave】ulellot~neallofhteport11porteinitearciotnins,~。。uslar~seeyaest11ybridsysteln.evedheess,」plleillnelw。15larl.dlanhtatweprctdsineyeasthyb`dsystelc~oslvehteproblen,chnlellleldal】erw.K”。n:1.sCaleyeasttweDhybirdsystenl,l、eininter。Ine11〕pign,Aan,~gn,Ubr~gn,,。,:2X(12`24,:2X()30226