HEMA与HEA辐射共聚制备固定化微生物载体的结构与性能李芳捷

Vol.22No.120093　　　　　　JournalofFunctionalPolymers　　　　:2008-09-22:(2006BAJ08B01-02);(863)(2003AA601100-4);(WTWER0707):(1984-),,,,。E-mail:[e-mail]:,E-mail:[e-mail]李芳捷1,　李正魁1,2,　杨竹攸1,2,　张晓姣1,　石鲁娜1(1.南京大学环境学院污染控制与资源化研究国家重点实验室,南京210093;2.南京大学水处理与水环境修复教育部工程研究中心,南京210093)　:　应用低温辐射技术辐射诱导甲基丙烯酸β-羟乙酯(HEMA)和丙烯酸羟乙酯(HEA)共聚合制备了高分子载体,用增殖细胞技术固定氨氧化细菌。利用红外光谱(FT-IR)、X射线光电子能谱(XPS)、X射线衍射(XRD)、扫描电子显微镜(SEM)以及接触角和含水率的测试对聚合物载体进行了性能表征。结果表明:经充分溶胀后的聚合物表面水接触角几乎为0,含水率为450%,润湿性能良好;聚合物表面具有极性官能团;聚合物的非晶结构有利于小分子尤其是水分子的渗透和扩散,多孔结构有利于微生物的生长和繁殖。以聚合物为载体固定化氨氧化细菌在处理含氨废水的过程中实现了短程硝化,在3种氨氮负荷(100、150、200mg/L)条件下,氨氮去除率和亚硝化率可分别达到95%和90%以上。:　辐射聚合;聚合物;亲水性;固定化;氨氧化细菌:　TQ317.3　　:　A　　:　1008-9357(2009)01-0017-06StructureandPropertiesofHEMAandHEACopolymerasCarrierforMicroorganismImmobilizationSynthesizedbyIrradiationMethodLIFang-jie1,　LIZheng-kui1,2,　YANGZhu-you1,2,　ZHANGXiao-jiao1,　SHILu-na1(1.StateKeyLaboratoryofPollutantControlandResourcesReuse,SchoolofEnvironment,NanjingUniversity,Nanjing210093,China;2.EngineeringCenterofWaterTreatmentandEnvironmentRemediationofMinistryofEducation,NanjingUniversity,Nanjing210093,China)Abstract:　Theammonia-oxidizingbacteriawereimmobilizedbyimmobilizedcellproliferationtechnologywithcopolymermadefromradiationcopolymerizationatlowtemperaturebymeansof2-hydroxyethylmethacrylate(HEMA)and2-hydroxyethylacrylate(HEA).FT-IRspectroscopy,X-rayphotoelectronspectroscopy(XPS),X-raydiffraction(XRD),scanningelectronmicroscopy(SEM),watercontactangleandwaterretentionwereusedtocharacterizethepropertiesofthecopolymer.Resultsshowthatwatercontactangleofcopolymerisalmost0andwaterretentionis450%aftersellingenough,whichindicatesthatcopolymerhasgoodsurfacewettability.Therearepolargroupsonthesurfaceofcopolymer.Thecopolymerisamorphous,infavourofinfiltrationandproliferationofsmallmoleculesespeciallywatermol-ecules.Thecopolymerhasholestructure,beingconducivetomicroorganismgrowthandreproduction.Theshort-cutnitrificationisrealizedusingimmobilizedammonia-oxidizingbacteriaduringwastewatertreatment.WheninfluentNH+4-Nloadofreactorhasgonethroughthreephrases(100、150、200mg/L),17DOI:10.14133/j.cnki.1008-9357.2009.01.014theammonianitrogenremovalrateandshort-cutnitrificationefficiencyreachmorethan95%and90%respectively.Keywords:　radiationcopolymerization;copolymer;hydrophilicity;immobilized;ammonia-oxidizingbacteria　　2060,。、、、、、,。Nilsson[1],20mg/L;[2],;Yang[3]。[4-5],,,,。-2,,NO-2-N[6]。β-(HEMA)(HEA)2,,。1　1.1　HEMA、HEA:,Fluka。。PHS-3CpH()、T52N()、THZ-82A()、60Co-γ()。1.2　HEMA、HEA,,-78℃,1×104J/kg、0.56J/(kg·s)60Co-γ。1.3　,5mm,,,。500mL,。1.4　NicoletNEXUS870,KBr,32,0.09cm-1。XRDRigakuD/Max-RAX-,CuKαX-(λ=0.154nm),2θ=4.5°～50°,40kV40mA,0.0017°/s。X(XPS)ThermoESCALAB250X-,AlKα(hυ=1486.6eV),200J/s,650μm,20eV。HITACHIS-3400N,,。18　　　　　　221.5　,20℃、40%,。,1μL。。,3,。(wc)。60℃,,,,,wc[7]:wc=(mh-mg)/mg×100%:mh,mg。1.6　,NH+4-N、NO-2-NNO-3-N,。,NH+4-N10gNaHCO3,(NH4)2SO4、、,pH8.0。NH+4-N(,2002);N-(1-)-NO-2-N(,2002);NO-3-N(,2002);(,2002);PHS-3CpHpH。2　2.1　HEMA-HEA[8],,。HEMA-HEA0,450%。,HEMA-HEA。2.2　HEMA-HEA1。3400cm-1O—H,1725cm-1C=O,1246、1167、1077cm-1C—O—C,C—H2950、2884cm-1,1451、1395cm-1。HEMA-HEA,,,。2。,,1650cm-11540cm-1,,2。,,[9]。1　HEMA-HEAFig.1　FT-IRspectrumofHEMA-HEApolymer2　HEMA-HEA(a)(b)Fig.2　FT-IRspectraofHEMA-HEAsupportbefore(a)andafter(b)immobilization191　,:HEMAHEA　　　　2.3　XPS,HEMA-HEAXPS,3a。HEMA-HEAC、O,C、H、O(H)。C1s、O1sN1s:63.96%,33.68%,2.36%,,。(O+N)/C0.56,,,。3bC1sXPS,C1sXPS5,284.8eVC—OC—H,286.3eVC—O,288.8eV—COOH,285.5eV288.5eVC—NC=O。XPS,,,。3　HEMA-HEAXPS(a)C1sXPS(b)Fig.3　XPSwidescan(a)andC1scorelevelspectra(b)ofHEMA-HEAcopolymer2.4　XRDSEM。,,,,,[10]。4HEMA-HEAX,,。,[11]。。[12]。[13-14],1～5,0.5～25μm。5HEMA-HEA,,20μm,。4　HEMA-HEAXRDFig.4　XRDpatternofHEMA-HEAcopolymer5　HEMA-HEASEMFig.5　SEMmicrographofHEMA-HEAcopolymer20　　　　　　22,,,,,。2.5　HEMA-HEA6a、。,3(100、150、200mg/L),,(95%90%)。6b180mg/L,3,,HEMA-HEA,,。HEMA-HEA,HEMA-HEA。6　HEMA-HEAFig.6　ActivitiesofHEMA-HEAimmobilizedammonia-oxidizingbacteriaa—Ammonianitrogenremovalefficiencyandshort-cutnitrificationefficiencywithdifferentammonianitrogenload;b—TimecoursesofNH+4-N、NO-2-NandNO-3-N3　　(1)HEMA-HEA,,,。(2)3,,,,。:[1]　NilssonI,OhlsonS,HäggströmL,etal.Denitrificationofwaterusingimmobilizedpseudomonasdenitrificanscells[J].AppliedMicrobiobiogyandBiotechnology,1980,10(4):261-274.[2]　.[J].,1986,22(2):27-31.[3]　YangPY,CaiTiande,WangMingli.Immobilizedmixedmicrobialcellsforwastewatertreatment[J].BiologicalWastes,1988,23(4):295-312.[4]　PashovaS,SlokoskaL.Physiologicalaspectsofimmobilizedaspergillusnigercellsproducingpolymethylgalacturonase[J].ProcessBiochemistry,1999,35(1-2):15-19.[5]　LauPS,TamNFY,WongYS.Effectofcarrageenanimmobilizationonthephysiologicalactivitiesofchlorellavalgaris[J].BioresoureTechnology,1998,63(2):115-121.[6]　,,,.[J].,2008,29(10):142-147.[7]　,,.(PVA)、[J].,2006(5):671-675.[8]　,,.[M].:,2000:38-40.[9]　HadjievD,DimitrovD,MartinovM,etal.Enhancementofthebiofilmformationonpolymericsupportsbysurfacecon-211　,:HEMAHEA　　　　ditioning[J].EnaymeandMicrobialTechnology,2007,40(1):840-848.[10]　,,,.[J].,1994,10(1):54-57.[11]　ChoJunSik,HanYounggun,JeromeJCuomo.Ionbe