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HPLC－ESI－MS2G*121213105003720500373430079G－。OctadecylC18－－20∶80∶0．005。5μg/LRSD%6．2%64．9%～76．3%。、G。GSimultaneousDeterminationofPENGinPharmaceuticalWastewaterbyHPLC－ESI－MS2*KANGQuan－ying12ZHUXiao－lei12ZHANGYuan131HebeiProvincialAcademyofEnvironmentalSciencesHebeiShijiazhuang0500372HebeiProvincialLaboratoryofWaterEnvironmentalScienceHebeiShijiazhuang0500373DepartmentofEnvironmentalEngineeringSchoolofResourceandEnvironmentalScienceWuhanUniversityHubeiWuhan430079ChinaAbstractAmethodwasdevelopedforthesimultaneousdeterminationofPENGinpharmaceuticalwastewaterbyhighperformanceliquidchromatography－electrospraytandemmassspectrometryHPLC－ESI－MS2．ThesamplewaspurifiedbyOctadecylC18solid－phaseextractioncolumn．ThehighperformanceliquidchromatographHPLCsepara-tionwasperformedwithacetonitrile－water－aceticacidvolumeratio20∶80∶0．005asmobilephase．Theidentifica-tionandquantificationweredonewithmassspectrometry．Thelimitofdetectionwas5μg/L．Therecoveryrangedfrom64．9%to76．3%withrelativestandarddeviation6．2%．TheexperimentalresultsshowedthatthismethodwasaccurateandrapidwhichcanbeusedfortheresidueanalysisofPENGinpharmaceuticalwastewater．KeywordsHPLCelectrospraytandemmassspectrometryPENGpharmaceuticalwastewater*No76711D。1980－。GpKa=2．75。β－。β－pH、β－、、1。2－3、4－5、、。－HPLC－MSHPLCMS、、、。ESI。ESI－MS2。－HPLC－ESI－MS2GG。、G。11．1LCQAdvantageESIThermoFinnigan1100C18OctadecylC18/18%500mg/6mg·L－1Agi-lent。、Gsigma·58·20113920MilliQ18．2MΩ·cm。1．2G50∶500．1mg/mL4℃。1．3EclipiceplusC183．5μm4．6mm×100mm30℃－－20∶80∶0．0050．2mL/min10μL254nm。1．4ESI5kV300℃15V30V40arb5arbFullScanMS、SIM、FullScanMS/MSm/z333m/z289100～800。1．54℃2。OctadecylC185mL5mL。5mL2mL/minC185mL2mL/min。5mL1mL/min0．45μm。22．1GESI－GM－Na+－m/z3331M－Na+－2。3。1G23G2．2－、。G0．05%。0．05%20∶80－－20∶80∶0．005。2．32．3．1100、200、400、600、1000μg/LG289GY=82．1159X－5569．610．99453G5μg/L。2．3．2G600μg/L6333289RSD%6．2%。100μg/L、400μg/LG·68·20113920G64．9%～76．3%。2．3．3。。6C18。371．3μg/L69．7%．3－HPLC－ESI－MS2G、、。Gβ－β－。β－。、G。1．J．20056112－116．2．5J．20017779－781．3．5J．200119672－74．4．－5J．2007261276－79．5．－GJ．20049238－11．6．M．2001檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵檵85．534Sr2+Sr2+。Sr2+2mg/L100mg/L79．8%6．7%96．6%15．1%。。3Sr2+。Sr2+Sr2+35%～40%Sr2+Sr2+15%～20%pHSr2+10%～40%Sr2+Sr2+10%～20%。/。1．Cu2+J．2007158－5962．2．Sr2+J．2011135－37．3．Cu2+J．20103812125－126136．4．Pb2+、Cu2+、Zn2+J．200514769－71．5．Pb2+J．2010371124－29．6．MnⅡJ．200831115－18．·78·20113920