MEL

©HachCompany,1993.Allrightsreserved.4-1-93-1EDforMEL/MPNTotalColiformandE.coliLaboratoryMEL/MFTotalColiformLaboratory25699-22MICROBIOLOGYENVIRONMENTALLABORATORIES—MELInstrumentandProceduresManualiiCONTENTSSectionPageSafetyPrecautions....................................................iv1.PortableIncubator..................................................1Specifications....................................................1GeneralDescription...............................................2PreparingforUse.................................................3Unpacking.................................................3Operation....................................................4PowerSelection............................................4OperatingControlsandIndicators..............................4Operation..................................................5Calibration.................................................5Maintenance6Cleaning..................................................6ReplacingtheFuse..........................................6ReplacementPartsandAccessories..................................7RepairService...................................................9Warranty...................................................102.MicrobiologicalProcedures..........................................11OverviewofColiformBacteria.....................................11TestingTechniques..............................................11PreparingSampleContainers......................................11UsingPresterilizedContainers................................11PreparingAutoclavableContainers............................12CollectingandPreservingSamples..................................12SampleSize...............................................12CollectingSamples.........................................13PreservingSamples.........................................13MUGReagentforE.coliScreening.................................13Presence/Absence(P/A)Procedures.................................14PotableWaterProcedures....................................14TotalColiformandE.coliProcedures....................14InterpretingP/AResults.....................................17ConfirmingPositiveSamples.................................17DisposingofCompletedTests................................18MediaandApparatusforP/ATesting..........................18RequiredMediaandApparatus..........................18OptionalMediaandApparatus..........................18MembraneFiltration(MF)Procedures...............................19PreparingMaterials.........................................19UsingPresterilizedEquipmentandMedia.................19UsingFieldFiltrationApparatus.........................19UsingAutoclavableEquipment.........................20SampleSize...............................................21DilutingSamples..........................................22DilutionTechnique...................................22DilutionSeries.......................................22PotableWaterProcedures....................................23iiiTotalColiformProcedure..............................23TotalColiformConfirmationProcedure...................25FecalColiformConfirmationProcedure...................27E.coliProcedures....................................28NonpotableWaterProcedures................................31TotalColiformTestforStressedOrganisms...............31FecalColiformProcedure..............................32FecalColiformConfirmationProcedure...................34E.coliProcedure.....................................35InterpretingMFResults.....................................38Media,ReagentsandApparatusforMFTesting..................39RequiredMediaandApparatus..........................39OptionalMedia,ReagentsandApparatus.................39MostProbableNumber(MPN)Procedures...........................41PotableWaterProcedures....................................41TotalColiformProcedure..............................41TotalColiformConfirmationProcedure...................43FecalColiformandE.coliConfirmationProcedure.........44NonpotableWaterProcedures................................46SampleSizeandDilution..............................46PreparingDilutionWater..............................46DilutionTechnique...................................46TotalColiformProcedure..............................49TotalColiformConfirmationProcedure...................51FecalColiformandE.coliConfirmationProcedure.........52FecalColiformProcedure..............................54InterpretingMPNResults....................................56Media,ReagentsandApparatusforMPNTesting................59RequiredMediaandApparatus..........................59OptionalMedia,ReagentsandApparatus.................60IllustrationsFigure1.PortableIncubatorControlsandIndicators..........................4TablesTable1.InterpretingP/AResults.........................................17Table2.ConfirmationMedia............................................17Table3.SuggestedSampleVolumesforMFTotalColiformTest..............