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MBR1,2,1,1(1., 100084;2., 150090):-(MembraneBioreactor,MBR).,.,MBR,.,(FluorescentinSituHybridization,FISH),MBR.021NⅡ,G2M.:-(MBR);;;(FISH):X172 :A :0250-3301(2004)-0056-03:(863)(2002AA601220):(1975～),,2001.MoleculeEcologyAnalysisofFilamentousBacteriumSludgeBulkinginMBRSUNYu-jiao1,2,WANGYong1,HUANGXia1(1.EnvironmentalSimulationandPollutionControlStateKeyJointLaboratory,DepartmentofEnvironmentalScienceandEngineer-ing,TsinghuaUniversity,Beijing100084,China;2.EnvironmentBiotechnologyResearchOfficeofHarbinIndustryUniversity,Harbin150090,China)Abstract:Membranebioreactor(MBR)isahotspottechnicsinwastewatertreatmenttechnologies.NowthestudyofMBRfocusesonmacroparametercontrolandsystemrunningeffectedbysludgecharacteristics,andtherearelittlestudiesonmoleculeecologyofactivesludge.Sludgebulkingfrequentlyinexperimentandlong-termpracticeanditsnegativeeffectonsystemoperationwasdiscoveredintheexperiment.Inordertoknowthebulkingprocess,fluorescentinsituhybridizationtechniquetoworkonthesludgeinMBRwasused.Itwasfoundthatthe021NⅡtypefilamentousbacteriumcausingsludgebulkingtheourexperiment,andthecontentoffilamen-tousbacteriumhybridizedbyG2Mprobearegrowinggradually.Keywords:membranebioreactor(MBR);filamentousbacterium;moleculeecology;fluorescentinsituhybridization(FISH) 2:,.,,,.MBR.MBR,,.90d,5～7g/L,1,SVI150,,.,COD80%,.,15d4d.MBR.MBR,,,MBR.(FluorescentinsituHybridization,FISH),,,,,.,DAPI,.FISH,,.1 1.1 2520046 ENVIRONMENTALSCIENCEVol.25,Sup.June,2004DOI:10.13227/j.hjkx.2004.s1.012,-.14L.,1.0.1μm,,0.2m2,12L/(m2·h),0.6m3/h.1 Table1 Thecompositionofinputwater/mg·L-1/mg·L-1278COD280～400278TOC110～12528BOD5150～300MgSO4·7H2O66NH+4-N36～72MnSO4·7H2O6pH6～8NaHCO311.1KH2PO45.28FeSO40.3CaCl26(NH2)2CO1671.2 ,,Eikelboom1975,[3].1.3 FISH[1],.Cy3,DAPI(4,6-diamidino-2-phenylindole),DAPI1μg/mL,5～10min..DAPI.2 (1).,,SVI100,,;SVI150,、,.,,.3 ,MBR.,,.3.1 1 Fig.1 MicroscopephotosofsludgeinMBR ,、、,.,,,.EikelboomMBR021N.021N[2],3021NⅠ、021NⅡ、021NⅢ.021NⅠ、021NⅡ.3.2 FISH(2),HGC,gama、beta,gama;G1B、G2M、G3M3021N,57 2 FISHFig.2 DominancefilamentousbacteriumexaminedbyFISH021NⅡ(G2M).FISHDAPI,,.4,021NⅡ(G2M)0%、2.8%、20%、55d,40%.,MBR021NⅡ(G2M),.,[4],...3 FISHFig.3 ThepicturesofcontentchangingoffilamentousbacteriumbyFISH4 FISHFig.4 Contentchangingoffilamentousbacterium4 ,-,gama021NⅡ,,,40%.021N,MBR.:[1] AmannRI.InsituidentificationofmicroorganismsbywholecellhybrizationwithrRNAtargetedmucleicacidprobes.In:MolecularMicrobialEcologyManual,part3.3.6KluwerAca-demic,Dordrecht,1995,1～15.[2] ArugaS,YKamagata,TKohno,SHanada,KNakamura,TKanagawa.CharacterizationoffilamentousEikelboomtype021NbacteriaanddescriptionofThiothrixdisciformissp.nov.andThiothrixflexilissp.JSystEvolMicrobiol,2002,52:1309～1316.[3] EikelboomDH.Filamentousorganismsobservedinactivatedsludge[J].WaterRes.,1975,9:365～388.[4] .[M].:,1988.203～204.58 25