MBR中厌氧氨氧化运行特性及微生态结构

第29卷第4期2010年7月食品与生物技术学报JournalofFoodScienceandBiotechnologyVol.29No.4Jul.2010:16731689(2010)04058108:20090415:973(2007CB714036);(BK2007022);(BS2007125)*:(1969-),,,,,,Email:[e-mail]中厌氧氨氧化运行特性及微生态结构路青1,华兆哲*2,李秀芬1,葛富青1,堵国成2,陈坚2(1.江南大学环境与土木工程学院,江苏无锡214122;2.江南大学工业生物技术教育部重点实验室,江苏无锡214122):研究了膜生物反应器(MBR)中厌氧氨氧化(Anammox)的运行特性与微生态结构变化采用含氮模拟废水进行试验,最终获得粒径集中在02~1mm的红褐色厌氧氨氧化颗粒污泥运行结果显示,厌氧氨氧化菌能够承受的容积负荷达0245kgTN/(m3d);总氮NH4+N和NO-2N去除率分别达到80%81%91%水力负荷冲击试验表明,当HRT从14h下降到79h时,NH4+NNO-2N去除率相对稳定,分别保持在75%和85%左右,厌氧氨氧化仍然能够稳定进行通过末端限制性酶切片断长度多态性(TRFLP)试验发现,反应器运行完成后微生物呈多态性分布,优势菌突出,其中起Anammox作用的菌属主要为planctomycepirellulagemmatapseudomonas,这为厌氧氨氧化运行过程中微生物群落结构变化提供理论依据:膜生物反应器;厌氧氨氧化;颗粒污泥;微生物种群分布:X703.1:AOperationCharactericticsandMicrobialCommunityDistributionofAnammoxinaMembraneBioreactorLUQing1,HUAZhaoZhe*2,LIXiuFen1,GEFuQing1,DUGuoCheng1,CHENJian2(1.SchoolofEnvironmental&CivilEngineering,JiangnanUniversity,Wuxi214122,China;2.KeyLaboratoryofIndustrialBiotechnology,MinistryofEducation,JiangnanUniversity,Wuxi214122,China)Abstract:TheoperationcharacteristicsandmicrobialcommunitydistributionofAnammoxprocessintheMBRwasstudiedinthismanuscript.Seedingwithsyntheticmedia,wefoundthatthegranularsludgewith02~1mmdiameterwasobtainedsuccessfully.Thenitrogenloadingrateamountedto0.245kgTN/(m3d),andtheremovalefficiencyoftotalnitrogen,NH4+NandNO2Nwas80%,81%and91%,respectively.Experiencingthehydraulicshocktext,theHRTofMBRwasgraduallyshortedfrom14to7.9h,theNH4+NandNO2Nremovalefficiencieswerekeptatabout75%and85%,respectively.Basedonterminalrestrictionfragmentlengthpolymorphism(TRFLP)analysis,Microbialcommunitydiversitywasachieved,andthepredominantpopulationsofAnammoxbacteriafoundinMBRsystemweretheplanctomyce,pirellula,gemmata,pseudomonasafterenrichment.TheresultspresentedhereprovidedatheoreticalbasisforthechangeofmicrobialcommunityduringAnammoxprocess.Keywords:membranebioreactor,anaerobicammoniaoxidation,granularsludge,microbialcommunitydistribution(Anaerobicammoniaoxidation,Anammox),NH4+N,NO2--N,[1],260kg/(m3d)[2],Anammox,95kg/(m3d)[3]Anammox[410],3[3]AnammoxAnammox[4],[510],[1113](MembraneBioreactor,MBR),,[14-15]Wyffels[16]MBRCanon(completelyautotrophicnitrogenremovalovernitrite),TrigoC[17]Anammox,MBR,MBRNO2-N,MBR,AnammoxMBR,Anammox,Anammox1材料与方法11MBRMBR1,1L,80mm,220mmU,01~02m,:pH76015,(321)!,1d,1h,225h,02h,03h1MBRFig.1Sketchofmembranereactorforanammoxenrichment12,(TS)148g/L,(VS)96g/L,VS/TS065,11[17]Tab.1Compositionofthesyntheticmedia/(mg/L)KHCO31000KH2PO410MgSO47H2O586CaCl22H2O565NH4ClNaNO2ZnSO47H2O430CoCl26H2O240CuSO45H2O250MnCl24H2O990Na2MoO42H2O220H3BO414NiCl26H2O190Na2SeO410H2O210:1ml/L582食品与生物技术学报第29卷13131物理性质测定1):,151080502mm,,105!,()2)(SVI)TSVS:132化学分析1)Anammox[18]:250mL,NH4+NNO-2N70mg/L,,2~3min,30!,NH4+NNO3-NNO-2N(TN)=NH4+N+NO-2NNO3-N2):3):N(1)4):5)pH:DELTA320pH133微生态结构分析1)DNA:3SDNA,TaKaRaDNA2)PCR:27F/1492R(50L):10∀PCRbuffer5L,MgCl22mmol/L,dNTPs120mol/L,20mol/L,1L,Taq25L,50L:94!5min,94!1min,72!1min,30,72!7min,4!TaKaRa3):PCRMspI/HhaI2,MspI(20L):10∀Taqbuffer20L,50~100ngPCR,01%BSA2L,25L,20L37!,65!10min,,12!,,,#Web()TRFLP[19]2结果与讨论21MBR211污泥质量浓度2MBR(TS)(VS)VS/TS,,;VS/TS,066077,2MBRFig.2TimecoursesofsludgeconcentrationintheMBR212污泥颜色,,,Anammoxc[20],,,Anammox[18,21],,213污泥粒径3,4,,MBR02~10mm,AnammoxAnammox106mg/kgTN/(m3d),MBRAnammox3Fig.3Diameterdistributionoftheinoculatedsludge583第4期路青等:MBR中厌氧氨氧化运行特性及微生态结构4Fig.4DiameterDistributionoftheincubatedsludge22MBR221氮素的变化AnammoxAnammox5~7,Anammox0~10d,,,NO-2N,NH4+N,NH4+N,NO-2NNO3N0,NO2-N0Anammox,12,NO-2N98mg/L5MBRFig.5ProfilesofNH4+NremovalintheMBR28,NO-2N21mg/L,,NO-2NNO-2NNH4+N,,10%~30%Anammox,NH4+NNO-2N,NO3-NAnammoxNO-2NNO3-NCO2,NO3-NNH4+NNO-2N(NH4+/NO-2N:025~2),Anammox[22]86d,NH4+NNO-2NNO3-N1∃114∃013,Anammox6MBRFig.6ProfilesofNO-2NremovalintheMBR7MBRFig.7ProfilesofnitrogenremovalintheMBR87,HRT14h,23d,Anammox0245kg/(m3d),80%,NH4+NNO-2N81%91%222抗负荷冲击能力NH4+NNO-2N70mg/L,10%,,8,(HRT)14h79h,,MBRNH4+NNO-2N75%85%,54h,NH4+NNO-2N50%61%HRT,584食品与生物技术学报第29卷NH4+NNO-2NAnammox,NO-2NNH4+N11~13,Anammox,HRT79h,MBR223pH值的变化pH9,pH,881,,NO-2N,,pHAnammox[1],Anammox,55dpH,848HRTMBRNH4+NNO-2NFig.8EffectsofHRTonammoniumandnitriteremovalsintheMBR23MBR10AB1370016SrDNAPCPHha%Msp%(TRFs),MBRMBR100,Sorenson&s(H&):H&=-∋(n/N)ln(n/N),n,N,2025,,VS/TS10,MBR,,(HRT14h79h),(),[23],MBRMBR119MBRpHFig.9ThechangesofpHintheMBR585第4期路青等:MBR中厌氧氨氧化运行特性及微生态结构1016SrDNAPCRFig.10The16SrDNAPCRamplifiedproductionafterenzymedigestionA:B:11MBRFig.11ComprisonofmicrobialcommunitiesbetweenbeforeandafterAnammoxprocessintheMBR586食品与生物技术学报第29卷11,Clostridia,Flavobacteria,planctomycepirellulagemmatapseudomonasBetaproteobacteria(37%445%)Gammaproteobacteria(6%171%),Chloroflexi(107%48%)Actinobacteria(178%4%)Planctomycetacia(0%95%),Anammoxplanctomycepirellulagemmatapseudomonas,[2425]AnammoxAnammox,3结语1),MBRAnammox,014kg/(m3d)HRTAnammox,HRT14h,0245kg/(m3d),80%,NH4+NNO2-N81%91%,MBR,Anammox2)MBR,100,02~1mmAnammox,MBRAnammox3)TRFLP,,,planctomycepirellulagemmatapseudomonas(References):[1]StrousM,HeijnenJJ,KuenenJG,etal.T