MUCT工艺全程硝化和短程硝化模式下反硝化除磷研究曾薇

3310201210ENVIRONMENTALSCIENCEVol．33No．10Oct．2012MUCT11211*1.1001242.100124MUCTC/N．MUCT180dDOHRT70%．90%91%60%88%．FISH2PAOs37%．CODCOD50mg·L－1．NO－2-NNO－3-N38%．．MUCTX703.1A0250-3301201210-3513-092011-11-212012-04-16NCET-11-089181020051210761974～[e-mail]*[e-mail]RemovalinaModifiedUniversityofCapeTownMUCTProcessTreatingDomesticWastewaterUnderNitrificationandNitritationZENGWei1WANGXiang-dong1ZHANGLi-dong2LIBo-xiao1PENGYong-zhen11.CollegeofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100124China2.InstituteofArchitecturalExplorationandDesignBeijingUniversityofTechnologyBeijing100124ChinaAbstractTheperformanceofdenitrifyingphosphorusremovalundernitrificationandnitritationwasinvestigatedinalab-scaleModifiedUniversityofCapeTownMUCTprocesstreatingrealdomesticwastewaterwithlowC/Nratio．Theexperimentalresultsof180daysshowedthatnitritationwasachievedatshorthydraulicretentiontimeHRTandlowdissolvedoxygenDOof0.3-0.5mg·L－1andanaveragenitriteaccumulationrateNARofabove70%wasachievedduringnitritationstage．TheMUCTprocessshowedagoodperformanceofdenitrifyingphosphorusremoval．Undernitritationmodetotalanddenitrifyingphosphorusremovalefficiencieswere90%and91%respectivelyundernitrificationmodetotalanddenitrifyingphosphorusremovalefficiencieswere60%and88%respectively．AlthoughphosphorusremovalperformanceundernitritationwasbetterthanthatundernitrificationtheresultsoffluorescenceinsituhybridizationFISHindicatedthatthepercentageofphosphorusaccumulatingorganismsPAOsinbiomasswasabout37%undertwomodes．TheeffluentCODconcentrationwasbelow50mg·L－1duringtheexperimentalperiod．BatchtestsofdifferentnitrifyingsludgesshowedthatthepercentageofdenitrifyingphosphorusremovalbacteriaDPBsinPAOsusingNO－2-NasanelectronacceptorundernitritationmodewasalmostthesameasthatusingNO－3-Nasanelectronacceptorundernitrificationmodewithanaveragepercentageof38%．Comparedwithnitrificationmodewithconventionalphosphorusremovalnitritationmodewithdenitrifyingphosphorusremovalhasasuperiorperformancetreatinglimitedcarbonsourcewastewater．KeywordsMUCTprocessdomesticwastewaternitrificationnitritationdenitrifyingphosphorusremovalpoly-βhydroxyalkanoatePHANO－2NO－3．O2“”50%30%50%1～4．、5．NO－2DOI:10.13227/j.hjkx.2012.10.03833C/N．2080PAOs6～8．1993Kuba9O2NO－3-N．2004Satio10NO－2-NdenitrifyingphosphorusremovalbacteriaDPBs．1112．DPBs/A/ASBR13～15．．A/A1617．MUCTA2O//．．MUCT．MUCTUCT18～20MUCT．MUCT．NO－2NO－3．．11.1MUCT1．70L．71213、425、6、7．、1、21∶1∶2∶3．4．．24L、．1.2.3.4.5.6.17.28.9.10.11.DO12.13.14.15.16.17.18.19.1MUCTFig．1SchematicdiagramofMUCTprocess．1R1R2121R3．2．1.2MUCT1．C/N．C/N41d0.86．A2O．ABL994mLA415310MUCT1Table1CharacteristicsoftherawwastewaterCOD/mg·L－186～256157.8NH+4-N/mg·L－154～9970.3NO－2-N/mg·L－10～0.10.004NO－3-N/mg·L－10.05～1.30.6P/mg·L－11.7～11.45.4C/N0.86～3.382.07pH7.13～7.427.316mLB．Ag·L－1MgSO4·7H2O2.0CaCl2·2H2O0.91.0．Bg·L－1FeCl3·6H2O1.5H3BO50.15CuSO4·5H2O0.03KI0.18MnCl2·4H2O0.12Na2MoO4·2H2O0.06ZnSO4·7H2O0.12CoCl2·6H2O0.15EDTA10pH7.0．1.30.45μmCOD、PO3－4-P、NH+4-N、NO－3-N、NO－2-N21．DOpHWTW-Multi-340i．nitriteaccumulationrateNARNAR=cNO－2-NcNO－2-N+cNO－3-N×100%1cNO－2-NcNO－3-NNO－2-NNO－3-Nmg·L－1．Tax1=ceffR1+can1+R2－cax11+R1+R2ceffR1+can1+R2－cax1R2－co1+R1×100%2Tax2=coR3+cax11+R1－cax21+R1+R3ceffR1+can1+R2－cax1R2－co1+R1×100%3Tax=Tax1+Tax24To=cax21+R1+R3－co1+R1+R3ceffR1+can1+R2－cax1R2－co1+R1×100%5R1R2R3canPmg·L－1cax11Pmg·L－1cax22Pmg·L－1coPmg·L－1ceffPmg·L－1Tax11Tax22TaxTo．1.4fluorescenceinsituhybridizationFISHAmann22FISH．4℃4%PFA2～3h．100W、50%4min50%、80%98%3min．FISH0.9mol·L－1NaCl、20mmol·L－1Tris/HCl、0.01%SDSFA2．46℃2h222～24．48℃18min．20．OLYMPUSBX61Image-ProPlus6.0．2FISH1Table216SrRNA-targetedoligonucleotideprobesusedinFISHanalysis5'-3'/%NIT3CCTGTGCTCCATGCTCCG40Cy3NitrobacterNSO1225CGCCATTGTATTACGTGTGA35Cy3Ammonia-oxidizingβ-ProteobacteriaNtspa662GGAATTCCGCGCTCCTCT35Cy3NitrospiraPAO462CCGTCATCTACWCAGGGTATTAAC35Cy3AccumulibacterPAO651CCCTCTGCCAAACTCCAG35Cy3AccumulibacterPAO846GTTAGCTACGGCACTAAAAGG35Cy3AccumulibacterEUB338GCTGCCTCCCGTAGGAGT—FITCEubacteriaEUB338ⅡGCAGCCACCCGTAGGTGT—FITCEubacteriaEUB338ⅢGCTGCCACCCGTAGGTGT—FITCEubacteria1EUB-mixPAOmixEUB-mixEUB338∶EUB338Ⅱ∶EUB338Ⅲ=1∶1∶1FAFAPAOmixPAO462∶PAO651∶PAO846=1∶1∶15153331.5MUCT73．5～11523～28℃Ⅵ1118℃．DO0.3～0.5mg·L－1．hydraulicretentiontimeHRT．sludgeretentiontimeSRTmixedliquidsuspendedsolidMLSS2000～3000mg·L－1．3MUCTTable3ExperimentalschemeofMUCTprocesstreatingdomesticwastewater/L·h－1HRT/hR1/%R2/%R3/%/℃SRT/dⅠ1～14d5.831210010020023～2810～15Ⅱ15～42d51410010025023～2825±5Ⅲ43～57d6.6710.510010030023～2825±5Ⅳ58～86d8.75810012030023～2825±5Ⅴ87～121d8.7588012030023～2825±5Ⅵ122～15111.6768012030018～2325±5Ⅶ152～1808.7588012030018～2325±5MUCT105d171d2526DPBsPAOs．Kaxmax/KomaxMUCTDPBsPAOs．．1MUCT4.5L25LSBR4.5L150min．231.5L．1DO＞4mg·L－12NaNO2NO－2-N15mg·L－13NaNO3NO－3-N15mg·L－1．325℃±1℃pH7.2～8.0．22.1MUCTMUCTNO－2-NNAR2．3．2、3180d72MUCTNO－2-NNARFig．2VariationsofNO－2-NineffluentandNARintheMUCTprocess．ⅠNAR2MUCTHRTHRTHRTnitriteoxidizingbacteriaNOB．38%31%3．Ⅱ、HRTSRT347%38%．．ⅢNARHRT10.5hNAR11%．ⅣHRT8h615310MUCT3MUCTNH+4-NTNFig．3RemovalofNH+4-NandTNint