O工艺污染物去除效果和污泥微生物群落的影响1

36220152ENVIＲONMENTALSCIENCEVol．36No．2Feb．2015OA/O11231*1.1000842.1000833.410082A/O．A、BO2～3mg·L－15～6mg·L－1．HＲT20hACOD72.5mg·L－1±14.8mg·L－1B68.7mg·L－1±14.6mg·L－1COD67.0%68.8%0.8mg·L－195%．BOD55mg·L－1．A/O．O454、A、B58.7%59.2%、14.7%12.7%10.8%12.4%．BNitrosomonas、NitrospiraPlanctomyces．AzospiraAcidovoraA．Novosphingobium、Comamonas、SphingobiumAltererythrobacter、、．A/O454X172X703A0250-3301201502-0604-08DOI10．13227/j．hjkx．2015．02．0312014-06-302014-09-202012ZX07201-005-06-011985～[e-mail]*[e-mail]enintheOxicPartsofA/OＲeactoronDegradationofOrganicPollutantsandAnalysisofMicrobialCommunityforTreatingPetrochemicalWastewaterDINGPeng-yuan1CHULi-bing1ZHANGNan2WANGXing3WANGJian-long1*1.InstituteofNuclearandNewEnergyTechnologyTsinghuaUniversityBeijing100084China2.SchoolofWaterＲesourcesandEnvironmentChinaUniversityofGeosciencesBeijing100083China3.CollegeofEnvironmentalScienceandEngineeringHunanUniversityChangsha410082ChinaAbstractEffectsofdissolvedoxygenDOonthebiodegradationoforganicpollutantswereinvestigatedusingA/Oreactorsforthetreatmentofactualpetrochemicalwastewater．TwoA/OreactorsDOwerecontrolledat2-3mg·L－1intheoxicpartsofreactorAand5-6mg·L－1ofreactorBwereoperatedinparallelforcomparison．ThenearlyahalfofyearoperationresultsshowedthattheeffluentCODinreactorA72.5±14.8mg·L－1wasslightlyhigherthanthatinreactorB68.7±14.6mg·L－1ataHＲTof20h．TheaverageCODremovalefficiencieswere67.0%and68.8%respectively．Theeffluentammoniumconcentrationwasmaintainedat0.8mg·L－1andapproximately95%ofammoniumremovalwasachieved．TheeffluentBOD5concentrationwaslowerthan5mg·L－1．ThisindicatedthattheorganicpollutantscouldbedegradedthoroughlybytheA/OprocesseswhichwereaffectedslightlybyDO．Ｒesultsof454pyrosequencinganalysisofthesludgeinoxicpartsshowedthatatthephylumlevelssequencesbelongedtoProteobacteriaPlanctomycetesandBacteroideteswereabundantwith58.7%and59.2%14.7%and12.7%10.8%and12.4%oftotalbacterialsequencesinreactorAandBrespectively．AmmoniumoxidationbacteriaNitrosomonasnitriteoxidizingbacteriaNitrospiraandobligateaerobicbacteriawerehighlyenrichedinreactorBwithhighDOlevelswhiletheanaerobicdenitrifiersAzospiraandAcidovorawerehighlyenrichedinreactorAwithlowDOlevels．TheidentifiedbacteriabelongedtogeneraNovosphingobiumComamonasSphingobiumandAltererythrobacterwerereportedtodegradePAHschloronitrobenzenepesticidesandpetroleumwhichcontributedtothedegradationofpetrochemicalwastewater．KeywordsdissolvedoxygenpetrochemicalwastewaterA/Oreactor454high-throughputpyrosequencingmicrobialcommunity2OA/O．、、．、1．/23．、/A/O、4．A/O．O．60%～80%5．6．7A2/OODO4.0mg·L－11.0mg·L－1COD．Guo8DO1.5～2.4mg·L－1A/O．DODO．．Ｒocher4549、、、．10．DOA/ODOA/O．11.11．A/OA．A、B．AO1、O245LA、O1O21∶2∶228L．AO．AODO2～3mg·L－1B5～6mg·L－123～26℃．1Fig．1Schematicdiagramoftheexperimentalset-up1.2、．3∶1．、、、．1．CODTNTP．11Table1CharacteristicsoftheinfluentCODTOCBOD5UV254237.3±67.654.8±5.676.6±23.520.0±3.90.68±0.628.6±5.01.7±0.61.5±0.21UV254mg·L－1．201342448h．HＲT30h100%．COD70%．2013520201394HＲT30h2013952013116HＲT20h．SＲT50d．506362～3d、COD．、BOD5、TN、TPCOD．0.45μm．O．、SMP、、3D-EEM．1.3BOD5、COD、、、11．TOCTNTOC-VCPH/CPN．．MLSS．DOYSI-550A．3D-EEMHitachiF-700012Ex200～400nmEm240～500nm5nm1200nm·min－1PMT700V．454．1.3.1DNADNAE．Z．N．A．DNAOMEGA．1.3.2PCＲDNA16SrＲNA27F533ＲV1～V3．5'-GCCTTGCCAGCCCGCTCAGAGAGTTTGATCCTGGCTCAG-3'454B27F．5'-GCCTCCCTCGCGCCATCAGNNNNNNNNNNTTACCGCGGCTGCTGGCAC-3'454A10PCＲ533Ｒ．PCＲ20μL0.4μmol·L－11μLDNA200nmol·L－1dNTP1×FastPfuBuffer．DNAPCＲ．PCＲ94℃2min95℃30s55℃30s72℃30s2572℃5min．2%．AxyPrepTMAXYGEN．1.3.3、DNAQuant-iTPicoGreendsDNAInvitrogenDNA30μLTE2XPicoGreen60μL．465～485nm515～575nmTurnerBiosystemsTBS-380．DNA．454GSFLXTitanium．0.03OTU．MOTHUＲUsearch4.0.38．Silva106．22.1DOA/OA/O．A、BAOMLSS3～4g·L－15～6g·L－1MLVSS/MLSS71%～75%．23、COD．HＲTA、B2．CODCODA/O．BOD55mg·L－1A/O．HＲT20hCOD30h．HＲT20hACOD72.5mg·L－1±14.8mg·L－1B68.7mg·L－1±14.6mg·L－1COD2A、BTable2AnalysisoftheeffluentfromreactorAandBHＲT/hCOD/mg·L－1BOD5/mg·L－1/mg·L－1/mg·L－1/mg·L－1/mg·L－1UV254A3077.2±14.52.7±1.11.2±1.49.2±2.111.7±2.10.8±0.30.61±0.022072.5±14.84.9±0.30.8±0.314.4±3.022.0±1.01.2±0.20.67±0.07B3078.9±17.03.4±2.41.0±0.610.1±2.312.5±2.91.0±0.30.63±0.022068.7±14.62.8±0.60.8±0.515.5±3.423.4±1.61.1±0.20.70±0.076062OA/O2、CODFig．2TimecourseofCODconcentrationsintheinfluentandeffluentandCODremovalefficiency3、Fig．3TimecourseofNH+4-NconcentrationsintheinfluentandeffluentandNH4-Nremovalefficiency67.0%68.8%TOC64.4%69.1%．DO．DOA．37063690%．2.2A、BCOD、NH+4-N、TNTP4．A、B．100%ACODA．CODO．OCOD．OTNAB4A、BCOD、NH4-N、TNTPFig．4ChangesinCODNH+4-NTNandTPconcentrationsatdifferentpartsofreactorsATNA．OO．ATN31%B26%BOATN．TN0.07mg·L－1．．A/O、．BTPA39%34%．1.0mg·L－1《》．2.3A/O、SMP13．SMPCOD5、SMPFig．5ChangesinSMPcompositionintheinfluentandeffluent30%SMPCOD70%～75%．A、BSMP5．8062OA/O．61.3%48.9%16.5%．A、BDOAB20%．3D-EEM3、SMP14．A/OSMP．AB．2.4DOO3A、BO454．AceChao1OUT．ShannonSimpsonOUT．ShannonSimpson．3DOBDO．31Table3CharacteristicsofbacterialcommunitydiversitybasedondiversityindicesＲeadsOTUsAceChao1ShannonSimpsonA174232331496441926.310.007B152002408563743926.500.005197%OTUsO6．Proteobacteria、Planctomycetes、Bacteroidet