PAC强化MBR工艺去除污水中微量药物效能与微生物群落变化研究

、[1-3]“”、[4-5]。[6-7]。ng/L～μg/L[8-13]。、72%～100%[14]。MBR[15]。MBRPAC[16]。PACMBR。SerranoPACMBRα-ALF1b、β-BET42A[16]。PACMBRCBZ、NAP、SMXNa-DCF4MBR。2。11.1498%。PACMBR100083PACMBRMBR、、4-PCR-DGGE2。MBRPACMBR425%、17%、22%16%。282α-、β-、γ-、δ-、、、PACMBR。MBRPAC。PCR-DGGEX703.1A1000-3770(2014)09-0039-[e-mail]@163.com40920149TECHNOLOGYOFWATERTREATMENTVol.40No.9Sep.,2014391.0g/L-20℃1。1.2MBR7.5LPVDF0.1μm0.03m2。/。PACMBRPAC-MBRMBRS-MBR0.15～0.2mm1.0g/L。1.31.3.1Waters2695waters2998SymmetryRP-C18150mm×4.6mm5μmWaters0.1%pH=4.0～4.5/=3:2v:v0.6mL/min30μL。1.3.2PCR-DGGEPAC-MBRS-MBR。1DNADNA、、K。DNA。DNADNATE1μLA260/A2801.8DNA。2PCRDNADNA338F/534R5'ACTCCTACGGGAGGCAGCAG3'/5'ATTACCGCGGCTGCTGG3'16SrDNAPCRDGGE5'GC。PCR94℃5min94℃45s55℃30s2572℃7min。PCR2%80V30min。3DGGEDGGED-codeBio-Rad35%～65%20μL。1×TAE90V60℃14～16h。1×SYBRGreen30minAlphaHP。4DGGE50μLTEDNAPCR。GCPCR。pGEM-TeasyPromegaJM109LB//IPTG/X-Gal37℃18h。T7/SP6PCR。NCBI。22.1PAC-MBRS-MBR4PAC-MBRS-MBRCOD、TN、TP96%、68%、54%91%、65%、52%。2PAC-MBR。2CBZ、Na-DCF、NAPSMX412。1Tab.1BasicinformationandphysicochemicalpropertiesoffourpharmaceuticalsCBZNa-DCFNAPSMX236318230253C15H12N2OC14H10Cl2NNaO2C14H14O3C10H11N3O3S298-46-415307-86-522204-53-1723-46-6CASpKalogKow13.94.154.151.7/5.62.450.703.180.601PAC-MBRFig.1RemovalefficienciesofpharmaceuticalsinthePAC-MBRprocess050100150200250300350400020406080100
%dCBZNAPNa-DCFSMX40940PAC-MBR10d4100%PACPAC460dPAC-MBRS-MBR。PAC80、160d0.8gPACPAC-MBR4S-MBR10%。S-MBRCBZ20%CBZCBZCBZ。S-MBRNa-DCF9%～46%NAP40%SMX20%～60%。PAC-MBRS-MBR243。3PAC-MBRCBZ、Na-DCF、NAPSMXS-MBR25%、16%、17%22%PAC。PAC-MBRPACPAC。2.2PAC-MBRS-MBR2.2.1PCR-DGGEPCR-DGGE4。42。。1、2、58、9、10、119、1018、19、20、26、2821、22、23、29、30。2.2.2PAC-MBRS-MBRDGGE、PCRNCBIPAC-MBRS-MBRα-、β-、γ-、δ-、、、。α-13、15、28、29、3013Mesorhizobiumsp.NL123AB636289100%153PAC-MBRS-MBRFig.3AverageremovalefficienciesofpharmaceuticalsinthePAC-MBRandS-MBRprocesses020406080100
/%CBZNa-DCFNAPSMX2S-MBRFig.2RemovalefficienciesofpharmaceuticalsintheS-MBRprocess050100150200250300350400020406080100
%d4PAC-MBRS-MBRDGGEFig.4DGGEfingerprintsofPAC-MBRandS-MBRsludgesamplesPACMBR41MethylobacteriumthiocyanatumNR-044792100%28HyphomicrobiumspFR750255100%[17]29HyphomicrobiumspFN868372100%30A/O-MBR[18]。β-4、8、10、11、20、22。4unculturedMethylovorusspJN601497100%[19]8Methylophilaceae100%1011SchlegelellaAY538708Ideonellasp.W51.KC248055100%20MethylobacillusspJF808844100%22Methylobacillussp.LF-1EU780432100%。γ-1、21、24。1PseudomonasJX502666100%Pseudomonas1-321PseudomonasplecoglossicidaJQ66054399%24PseudomonaspsychrophilaJQ968688100%SMX。δ-5unculturedPolyangiaceaeFJ933315100%MBR。2、3、1923BacteroidetesbacteriumDQ337558)EU283401100%3MBR[20]19HM197738100%。725。7JX491360100%25SphingobacterialesbacteriumJN67920099%2MBR。27Arthrobactersp.Ib12JF928556100%。6、9、12、14、16、17、18、2326。69121416PAC1823[21]。PAC-MBRS-MBR13MBR18-2142832127/30。PAC-MBRS-MBR6、14、16、22、23、24。3PACMBRCBZ、NAP、Na-DCFSMXMBRPACMBRSMX。PACMBR。[1]Auerbach,EricA,Seyfried,etal.Tetracyclineresistancegenesinactivatedsludgewastewatertreatmentplants[J].WaterRes.,2007,41(5):1143-1151.[2]Bound,JonathanP,Kitsou,etal.Householddisposalof40942pharmaceuticalsandperceptionofrisktotheenvironment[J].EnvironmentalToxicologyandPharmacology,2006,21(3):301-307.[3]AuerbachEA,EESeyfried,KDMcMahon.Tetracyclineresistancegenesinactivatedsludgewastewatertreatmentplants[J].WaterRes.,2007,41(5):1143-1151.[4]Glassmeyer,STFurlong,EdwardTKolpin,etal.Transportofchemicalandmicrobialcompoundsfromknownwastewaterdischarges:Potentialforuseasindicatorsofhumanfecalcontamination[J].EnvironmentalScience&Technology,2005,39(14):5157-5169.[5]RichardsonSD,TATernes.Wateranalysis:emergingcontaminantsandcurrentissues[J].AnalyticalChemistry,2005,77(12):3807-3838.[6]Kasprzyk-Hordern,Barbara,Dinsdale,etal.Theremovalofpharmaceuticals,personalcareproducts,endocrinedisruptorsandillicitdrugsduringwastewatertreatmentanditsimpactonthequalityofreceivingwaters[J].WaterRes.,2009,43(2):363-380.[7]Lindqvist,Niina,Tuhkanen,etal.Occurrenceofacidicpharmaceuticalsinrawandtreatedsewagesandinreceivingwaters[J].WaterRes.,2005,39(11):2219-2228.[8]Gabet-GiraudV,etal.Occurrenceandremovalofestrogensandbetablockersbyvariousprocessesinwastewatertreatmentplants[J].SciTotalEnviron.,2010,408(19):4257-4269.[9]ZoritaS,LMartensson,LMathiasson.OccurrenceandremovalofpharmaceuticalsinamunicipalsewagetreatmentsysteminthesouthofSweden[J].SciTotalEnviron.,2009,407(8):2760-2770.[10]VienoNT,Tuhkanen,LKronberg.EliminationofpharmaceuticalsinsewagetreatmentplantsinFinland[J].WaterRes.,2007,41(5):1001-1012.[11]YingGG,RSKookana,DWKolpin.Occurrenceandremovalofpharmaceuticallyactivecompoundsinsewagetreatmentplantswithdifferenttechnologies[J].JEnvironMonit,2009,11(8):1498-1505.[12]ChangH,etal.Occurrenceofandrogensandprogestogensinwastewatertreatmentplantsandreceivingriverwaters:comparisontoestrogens[J].WaterRes.,2011,45(2):732-740.[13]SuiQ,etal.Occurrenceandremovalofpharmaceuticals,caffeineandDEETinwastewatertreatmentplantsofBeijing,Chi