pH对高氨氮渗滤液短程生物脱氮反硝化过程动力学的影响

30420104ActaScientiaeCircumstantiaeVol.30,No.4Apr.,2010:(No.50978003);(No.8091001)SupportedbytheNationalNaturalScienceFoundationofChina(No.50978003)andtheBeijingNaturalScienceFoundation(No.8091001):(1976),,,E2mail:[e-mail];3(),E2mail:[e-mail]:SUNHongwei(1976),male,Ph.D.,E2mail:[e-mail];3Correspondingauthor,E2mail:[e-mail],,,.2010.pH[J].,30(4):742-748SunHW,WangSY,WeiDY,etal.2010.TheeffectofpHonthekineticsofdenitritationinnitrogenremovalvianitritefromlandfillleachatewithhighammoniaconcentration[J].ActaScientiaeCircumstantiae,30(4):742-748pH1,1,2,1,1,1,1,31.,1001242.,510655:2009207227:2009212213:2010202226:pHNO-22N,/UASB2SBR,SBR(120d),SBRNO-22N(51020406080100mgL-1)pH(6.57.08.08.5),,pHNO-22N.,pH,pH6.57.08.5(k)pH8.049%61%63%;4pH,NO-22NMonod,pHMonod,(Ks)(k),pH8.0Ksk,15.8mgL-10.435gg-1d-1.:;;;;pH:025322468(2010)042742207:X703.1:ATheeffectofpHonthekineticsofdenitritationinnitrogenremovalvianitritefromlandfillleachatewithhighammoniaconcentrationSUNHongwei1,WANGShuying1,WEIDongyang2,SHIXiaoning1,ZHANGShujun1,YANGQing1,PENGYongzhen1,31.KeyLaboratoryofBeijingWaterQualityScienceandWaterEnvironmentRecoveryEngineering,BeijingUniversityofTechnology,Beijing1001242.SouthChinaInstituteofEnvironmentalSciences,SEPA,Guangzhou510655Received27July2009;receivedinrevisedform13December2009;accepted26February2010Abstract:TheeffectofpHonthekineticsofdenitritationusingnitriteaselectronacceptorwasinvestigatedinthisstudy.Alab2scaleUASB2SBRsystemwasoperatedtoachievenitrogenremovalvianitritefromlandfillleachate.StablenitritationwassuccessfullyachievedintheSBRafter120days.Subsequently,batchtestswerecarriedouttoassesstheeffectofpHonkineticconstantsofdenitritationusingthesludgefromSBRatdifferentNO-22Nconcentrations(5,10,20,40,60,80and100mgL-1)andatdifferentpHgradients(6.5,7.0,8.0and8.5).ThepHhasasignificantinhibitoryeffectontheactivityofdenitrifyingbacteria.Comparedtothemaximumspecificnitritereductionrate(k)atpH8.0,thenitritereductionratesatpH615,7.0and8.5decreasedapproximately49%,61%and63%,respectively.TherelationbetweenthenitritereductionrateandtheinitialnitriteconcentrationfollowedtheMonod2typeequation.Thelengthofthefirst2orderportionintheMonod2typecurvewasdistinct,resultingindifferentKsandkatpH6.5,7.0,8.0and8.5.ThehighestKsandkatpH8.0werecalculatedtobe15.8mgL-1and0.435gg-1d-1,respectively.Keywords:landfillleachate;partialbiologicalnitrogenremoval;denitritation;kinetic;pH1(Introduction),,(Garridetal.,1997).,:(DO)(Garridetal.,1997;Ruizetal.,2003);(SRT)(Hellingaetal.,1998);4:pH(Hellingaetal.,1998;Fuxetal.,2002;Mosquera2Corraetal.,2005;vanDongenetal.,2001);(FA)(FNA)(vanDongenetal.,2001;Anthonisenetal.,1976;Kimetal.,2006;Vadiveluetal.,2006;Balmelleetal.,1992;Vanetal.,2004;Fuxetal.,2003;Yamamotoetal.,2006);(Pengetal.,2007;Gaoetal.,2003).SHARON(Fuxetal.,2002)SBR(Yangetal.,2007).,,,.,,,,,,(max),(YN),(Ks),(Ko2)(k)(MetcalfEddy,2003)..(NO-22NN2),(NH+42NNO-22N),.,IWAASM,20,pH,,,,.,,,,,.,/UASB2SBR,,SBR,pHNO-22N.,pH,NO-22N,,Monod,,pH.2(Materialsandmethods)2.1/UASB2SBR,1.1/UASB2SBRFig.1Schematicoftheanoxic/anaerobicUASB2SBRbiologicalsystem,/UASB,SBR.SBRUASB,.UASBSBR,2.,UASB:302,(HRT)1d,6.5kgm-3d-1,31.SBR:(15.231.4),,DO,ORPpH,:30min,:30min.DO1.0mgL-1,.SBR4,(3.1).,.,SBR7L,72L.NO-22N.NaNO210mgL-1NO-22N,,NO-22N51020406080100mgL-1,,34730COD/NO-22N56.015molL-1HClpHpH0.05.2.2,:pH7.88.9;COD10388091mgL-1;TN10052444mgL-1;NH+42N8902360mgL-1.:100mL,0.45mMilliporefilter,105,MLSS.600,MLVSS.NH+42NNO-22NCOD(APHA,1995).WTW(pH/Oxi340i,WTW)DOORPpH.rDN,pH/rDN,8.0,pH8.0,pHpH8.0(Zhouetal.,2007).3(Results)2SBRFig.2NitriteaccumulationratioattheendofSBRnitrification3.1SBR2,SBR36d(4258),43.6%94.5%,.3(58728),90%,SBR..FISH,(AOB)3.6%,(NOB)013%,AOB(Yangetal..,2007;Pengetal.,2008).,SBRAOB,.3.2NO-22NpH(6.57.08.08.5)NO-22N(51020406080100mgL-1),NO-22N,.,pH.:(270.4),VSS(1.250111)gL-1.3NO-22N60mgL-1,4pH,NO-22N.,pH0.05,,,,4pHNO-22N(rDN),1.1pHNO-22NTable1ThespecificdenitritationrateofnitriteatfourdifferentpHpH6.57.08.08.5rDN/(gg-1d-1)0.2280.2840.4350.292:NO-22N60mgL-1,4pH,NO-22N;pHrDN.pH=8.0,rDN,0.435gg-1d-1,rDNpH.,SBR,,SBRpH7.59.0(),pH.(2006)UASB+A/O,A/O1pHUASB28.128.68,100%.,.,,20,pH7.0,NO-22N0.216gg-1d-1(,2002),pH8.0rDN50%.4pH.pH,NO-22N,NO-22N,4474:pH34pHNO-22N(NO-22N60mgL-1)Fig.3NO-22NconcentrationovertimeatfourdifferentpHvalues(initialNO-22Nconcentrationis60mgL-1).NO-22N,.,pH6.57.08.5rDN49%61%63%(pH8.0),pH,pH.4pHFig.4EffectofpHontheactivityofnitritereductionbacteria3.3pHNO-22N3.2,pH6.57108.08.5rDNNO-22N,5.,4pH,rDNNO-22N2,NO-22N,,rDN,,rDN,Monod(Monod,1949).5pHNO-22NFig.5ThekineticcurvesofdenitrificationusingnitriteaselectronacceptoratdifferentpH(2007)pH8.0,rDNNO-22NMonod,,rDNNO-22N.,pH8.03pH,54730NO-22N(Ks)(k).pH8.0,Ksk.,4pH,pH8.0pH.pH6.5,,.2pHTable2ThekineticequationsofdenitrificationatdifferentpHpHR26.5y=-2944-0.182exp(-x/10.45)+2944exp(-x/6.63)0.9857.0y=0.353-0.117exp(-x/3.55)-0.242exp(-x/48.72)0.9998.0y=0.459-0.043exp(-x/0.122)-0.413exp(-x/25.49)0.9988.5y=2028-0.194exp(-x/6.91)-2028exp(-x/1.84)0.986:yNO-22N(gg-1d-1);xNO-22N(mgL-1)3.