pH对间歇进水序批式生物反应S省略性污泥沉降性能和微生物结构的影响璩绍雷

35320163ENVIＲONMENTALCHEMISTＲYVol．35No．3March20162015731ＲeceivedJuly3120115．*07JCZDJC02100．SupportedbyAppliedBasicＲesearchProjectofTianjin07JCZDJC02100．＊＊[e-mail][e-mail]10．7524/j．issn．0254-6108．2016．03．2015073101．pHSBＲJ．2016353508-515QUShaoleiSUNBaoshengZHAOShuanghongetal．pHactivatedsludgesedimentationperformanceandthestructureofthemicrobeofSBＲtechnologyJ．EnvironmentalChemistry2016353508-515pHSBＲ*＊＊300072pHSBＲpH5．0—10．0．SVI．pH5．06．0．pHDNAPCＲShannon．pH．pHSBＲ．pHactivatedsludgesedimentationperformanceandthestructureofthemicrobeofSBＲtechnologyQUShaoleiSUNBaosheng＊＊ZHAOShuanghongZANGXiangrongLIZhijingWEIJiahongSchoolofEnvironmentalScienceandEngineeringTianjinUniversityTianjin300072ChinaAbstractThispapermainlystudiedtheinfluenceofpHonSBＲsludgebulkingandmicrobialstructurewiththepHvaluesoftheinflowwaterfrom5．0to10．0．TheresultsshowedthatthedidnotchangeinitiallyofSludgeVolumeIndexSVIvalue．HoweverthesludgebulkingwasseenovertimewhenpHvaluesbecame5．0and6．0．AtvariouspHvaluesthetotalbacterialDNAgroupswereextractedforPCＲamplificationandthenrelevantanalysesweregivenaboutShannondiversityindexclonedsequencingandphylogenetictreeofpartialdominanttotalbacteriainthemicrobialcommunities．ThisstudydemonstratedthatthestructuresandquantitiesofmicroorganismsweredifferentatdifferentpHvaluesinacidicconditionsfilamentousbacteriabecamedominantandtheyweremoreaccessibletosludgebulkinginneutralandalkalineconditionwhilezoogloeawerethedominantonesandtheactivityofsludgewasrelativelystable．KeywordspHSBＲtechnologyactivatedsludgemicrobialstructure．．1．FilamentousbulkingNon-filamentous3pHSBＲ509bulking．90%．pH6．0—8．0pH6．5．．pHpH5．0—10．0．pHSBＲpHSBＲpHSBＲpH-PCＲ-DGGEpH．1Materialandmethods1．1SBＲ14L6∶42—4mg·L－1．22℃—24℃．．．1Fig．1Textreactionequipment1．224hCOD、、MLSSSVISVI90mL·g－1COD、、85%．SBＲpH5．0—10．0．4000mL·g－1pHpH．．12hC6H12O6、NH42SO4、KH2PO3BOD5∶N∶P=100∶5∶1MgSO4、CaCl2、FeCl3．Cl－Na+2．0．5mol·L－1HClNaOHpH5．0、6．0、7．0、8．0、9．0、10．0．1COD650—51035820mg·L－17．5—8．2mg·L－140．97—49．46mg·L－145—54．5mg·L－1．1Table1ThecomponentsandconcentrationsofartificialdistributionwaterComponentC6H12O6NH42SO4KH2PO3MgSO4·7H2OCaCl2·2H2OFeCl3·6H2OConcentration/mg·L－180022036501048h4h12hpH．COD、MLSS、SV、SVI．20dpH20dpH．pH．1．3DNA1．3．1pH30mL50mL3．1．3．2DNA-//-DNA5mL4mL37℃30min10%SDS55℃1h9165r·min－110min///20．13mol·L－1－20℃2h6—10℃、9165r·min－110min70%100TEDNAA260/A280A260/A230DNA0．7%70V30min．4．1．4PCＲDNAPCＲ16SrDNAV3F357-GC5'-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCCCCTACGGGAGGCAGCAG-3'“GC”5Ｒ5185'-ATTACCGCGGCTGCTGG-3'．240bp．50μL10—100ng25μL2×TaqPCＲMasterMix1μL25μmol·L－1、50μL．PCＲ6．1．5PCＲDGGEC．B．S．SCIENTIFICDGGE－2001PCＲ7．1．6Shannon-WeaverQuantityOneDGGEShannonSBＲ．HH=－∑si=1PilgPi=－∑si=1ni/N()lgni/N()Pi=ni/NniN．2Ｒesultsanddiscussion2．1Ｒob83pHSBＲ511、3．SVIpH．SVI70—100mL·g－1．pHEPS9．pHpH．2SBＲSVIpH5．06．070mL·g－120dSVI170mL·g－1、．pHpH7．0—10．0SVI67—85mL·g－1pH=10．0SVIpH5．06．0．、、pH5．06．0．pH．pHpH．2Fig．2ThechangeofsludgeconcentrationandsludgevolumeindexpHpH5．06．0pH=7．0pH8．0—10．0pH5．0、6．0．EPS9．2．2DGGEpH20dpHpHpHpHpH．pH=10．0C1pH=9．0C2pH=8．0C3pH=7．0C4pH=6．0C5pH=5．0C6．pHPCＲDGGE3pHpH．512353pHDGGEFig．3DGGEprofilesoftotalbacterialunderdifferentpHpHDGGE32．2pHDGGETable2DGGEprofilesbandclassificationoftotalbacterialunderdifferentpHGraphfeatureTypicalstripepHBand156910pHBand171819pHBand234pHBand111214pHBand7131516pHBand83pH．pHBand6Band17、18、19pHBand2、3、4pHBand12pHpHBand16α-Band8．2．333Uncu-lturedbacterium．Band4Band18Ｒhodanobactersp．．Band3、12、16、17、19ProteobacteriaProteoba-cteria．Band3、3pHSBＲ51312、19γ-Gammaproteobac-teriumBand16α-alphaprote-obacteriumα-FISHα-10—1213—15pH=5．0．Band6Chloroflexi16．1718pH=6．0Band6pH．16SrDNA97%94%．5691%90%Unculturedbacteriumclone11-43UnculturedChlor-oflexibacteriumisolateDGGEgelBand4．DNAMANGenbank4．316SrDNATable316SrDNASequencinganalysisofpartofthemicrobialcommunityofthedominantbacteriaBandlength/bpGenbankGenbankcomparethesequenceaccessionnumbersimilarspecieshomologyBand2123GQ8711UnculturedbacteriumcloneUTFS-Ｒ12-214-10699%Band3123JX51225Unculturedgammaproteobacteriumisolate98%DGGEgelbandPHAs-29Band4149KF4474Ｒhodanobactersp．7B-39399%Band5154GU5606Unculturedbacteriumclone11-4390%Band6132HM1640UnculturedChloroflexibacteriumisolateDGGEgelBand491%Band8162EU6383UnculturedZoogloeasp．cloneSHBZ77599%Band9161HQ6579UnculturedＲhodocyclaceaebacteriumclonehrb-11899%Band10116GQ8791UnculturedbacteriumcloneUTFS-Ｒ12-214-9597%Band12125HQ8268UnculturedgammaproteobacteriumcloneN1199%Band13133CU9148UnculturedTM7bacterium97%Band14150JN67916UnculturedXanthomonassp．clone5．17m24100%Band16125GQ3540UnculturedalphaproteobacteriumcloneMS11-2098%Band17122EU2916UnculturedproteobacteriumcloneGASP-KC2S2_C0698%Band18149KF7365UnculturedＲhodanobactersp．clone11．17_7_898%Band19122EU2806UnculturedgammaproteobacteriumcloneAS13798%2．4Shannon-WienerpH．C1—C6Shannon-WienerC1=0．942C2=0．943C3=1．06C4=0．946C5=0．928．pHpH8．0Shannon1．06pH7．0、9．0、10．0ShannonpH=6．0pH=5．0pH7．0、9．0、10．03pH．3Conclusion1pH．pH．．514354DNABLASTFig．4Phylogenetictreeoftotalbacteriasystemaccordingtoth