pH和温度对氯胺消毒给水管网硝化作用的影响周玲玲

32620116ENVIRONMENTALSCIENCEVol．32No．6Jun．2011pH12*222131.2000922.2000923.150090RABpH．15℃30℃．15℃pH30℃pH．pH7.5±0.229.3±0.240.76mg/LAOB0.6lgMPN/cm21.4lgMPN/mL98CFU/mL．2pH43．15℃pH7.4±0.159.3±0.26、．pHX52TU991.25A0250-3301201106-1627-052010-06-052010-09-06508781642008ZX07422-005YRWEY1001201004806261976～[e-mail]*[e-mail]reonNitrificationinaBench-ScaleDrinkingWaterDistributionSystemDuringChloraminationZHOULing-ling1ZHANGYong-ji2SONGZheng-guo2ZENGGuo2YEHe-xiu2GAONai-yun1LIGui-bai31.StateKeyLaboratoryofPollutionControlandResourcesReuseCollegeofEnvironmentalScience＆EngineeringTongjiUniversityShanghai200092China2.KeyLaboratoryofYangtzeRiverWaterEnvironmentMinistryofEducationTongjiUniversityShanghai200092China3.SchoolofMunicipalandEnvironmentalEngineeringHarbinInstituteofTechnologyHarbin150090ChinaAbstractTheeffectofpHandtemperatureonthecontrollingofnitrificationwasevaluatedusingrotatingannularbioreactorsRABswhichwereappliedtosimulatethedrinkingwaterdistributionsystem．TheresultsshowedthatelevatedpHhadlittleeffectonnitrificationoccurrenceatlowtemperature15℃howeverthepHcontrollingeffectonnitrificationwasobviousathightemperature30℃．WithpHincreasingfrom7.5±0.22to9.3±0.24athightemperature30℃theresidualchloraminesconcentrationincreasedto0.76mg/LandtheAOBconcentrationinthebiofilmandbulkwaterreduced0.6lgMPN/cm2and1.4lgMPN/mLrespectivelytheheterotrophicbacteriaconcentrationinbulkwaterreduced98CFU/mL．WithpHincreasingfrom7.4±0.15to9.3±0.26comparedwiththeinfluentnitriteconcentrationtheeffluentnitriteconcentrationincreasedfourandthreetimesrespectivelyatdifferentpHlevels．Theeffluentresidualchloraminesnitritenitrogenammonianitrogenconcentrationsvariedslightlycomparedwithinfluentatlowtemperature15℃andtheeffluentheterotrophicbacteriaconcentrationwasalsostable．KeywordspHchloraminesdrinkingwaterdistributionsystemnitrificationammonia-oxidizingbacteriaAOB12．、．63%．HPC3．AOBAOB、、、pH4．pHAnnArborpH9.35．pHAOBDOI:10.13227/j.hjkx.2011.06.01832AOBpH56．RABpHAOB2．11.1RAB7．RAB800mL53～160min5～15mL/min．RAB2018.2cm250～150r/min．10mg/L24h3．1.21.2.1MPN11∶5RAB4．．1MPNTable1CompositionoftheMPNenumerationmediumNH42SO4/g0.50KH2PO4/g0.20MgSO4·7H2O/g0.04CaCl2·2H2O/g0.04Fe-EDTA1/mL10.5%/1/mL10001EDTA1.2.2NaClODPDNH4ClNH+4-NN．pH=8.0NaClONH4Cl3∶115minDPD．1.2.32RAB415℃30℃83.5mg/L．pH7.5±0.44pH9.3±0.34pH1mol/LNaOH．pH、、、AOBHPC2．2、pHTable2Two-levelfactorialexperimentaldesigntemperaturepH/℃pH=7.5±0.4pH=9.0±0.330R1R115R2R21.31.3.1HPCHPCR2A7．1.3.2AOBAOBSorianoWalker14MPN8．12345mL1、0.10.01mL28℃21d9．0.6%-α-0.8%300μL5minNO－2-NAOB2．1.3.3NH+4-NNO－2-NN-1--752N．DPDNN--14-10．22.1pH130℃15℃pH7.59.32．1pH30℃15℃．30℃pHpH7.59.30.098±0.031mg/L0.068±0.018mg/L2pH43．0.05～0.5mg/L82616pH0.05mg/L1112．R1pH．15℃R20.05mg/L．pH．1pHNO－2-NFig．1EffectsofpHandtemperatureonNO－2-Nineffluencewater2.2pH2pHR1R2．2pH30℃pH7.59.30.59±0.23mg/L1.35±0.15mg/L15℃1.01±0.11mg/L1.15±0.10mg/L．pHpH9.3．．．pH9.3pHpH．2.3pHAOB30℃pHAOB3．pH7.5±0.222pHFig．2EffectsofpHandtemperatureoneffluentchloraminesconcentrationAOB2.0lgMPN/cm21.5lgMPN/mL4AOB0.1lgMPN/cm20.3lgMPN/mL．pH9.3±0.24AOBpH7.5±0.220.6lgMPN/cm21.4lgMPN/mL．12pHAOBpH13～17．3pHAOBFig．3EffectsofpHonbiofilmandeffluentAOBconcentration2.4pH3pH．15℃2pH．30℃pHpH7.50.04mg/LpH9.30.26mg/L．、pH．pH926132pHpH18．．3NH+4-N/mg·L－1Table3AverageNH+4-NconcentrationofinfluentandeffluentunderdifferentpHconditions/mg·L－115℃30℃pHpHpHpHpHpHpHpH0.320.240.330.280.540.510.580.772.5pHHPC4pHHPC．4HPC．2pHHPC261±94CFU/mL193±67CFU/mL134±34CFU/mL129±38CFU/mL．pHHPCpHHPC．2pHHPC．4pHHPCFig．4EffectsofpHandtemperatureoneffluentHPCconcentration3AOBAOB30℃20℃2～35℃20℃1/3AOB．pHAOBpHAOBpH1920pHAOBAOB5．15℃pHpH．pH7.59.3pHAOBpHAOB21～24AOBpHAOB．pHAOBAOBpH．41．2pHpH．3pHHPCpHHPC．4pH．1CunliffeDA．BacterialnitrificationinchloraminatedwatersuppliesJ．AppliedandEnvironmentalMicrobiology199157113399-3402．2WolfeRLLieuNIIzaguirreGetal．Ammonia-oxidizingbacteriainachloraminateddistributionsystemseasonaloccurrencedistributionanddisinfectionresistanceJ．AppliedandEnvironmentalMicrobiology1990562451-462．3OdellLHKirmeyerGJWilczakAetal．ControllingnitrificationinchloraminatedsystemsJ．AmericanWaterWorksAssociationJournal199688786-98．4WilczakAJosephGJacangeloJPetal．OccurrenceofnitrificationinchloraminateddistributionsystemsJ．AmericanWaterWorksAssociationJournal199688774-85．03616pH5SkadsenJ．EffectivenessofhighpHincontrollingnitrificationJ．AmericanWaterWorksAssociationJournal200294773-84．6HarringtonGWNogueraDRKandouAIetal．Pilot-scaleevaluationofnitrificationcontrolstrategiesJ．AmericanWaterWorksAssociationJournal2002941178-89．7．J．200829123372-3375．8SorianoSWalkerN．Isolationofammonia-OxidizingautotrophicbacteriaJ．JournalofAppliedBacteriology1968314493-497．9APHA．StandardMethodsfortheExaminationofWaterandWastewaterM．20thed．WashingtonDCUSAAmericanPublicHealthAssociation1998．10．M．．2002.172-177．11ReganJMHarringtonGWNogueraDR．Ammoniaandnitrite-oxidizingbacterialcommunitiesinapilot-scalechloraminateddrinkingwaterdistributionsystemJ．AppliedandEnvironmentalMicrobiology200268173-81．12KirmeyerGJOdellHJJac