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pHSBBR12221．4000742．400045pHSBBRDNAAOB、NOBANAMMOXPCRpH。pHSBBR。pH=6．0AOB、NOBANAMMOXpH。AOBpH=7．0～8．0pH=9．0。pH=7．0～9．0NOBAOB。pH=8．0SBBR。SBBRpHPCRX703A1000－4602201217－0036－0450608071308020CSTC2011jjA20014EffectofpHonSBBRAutotrophicNitrogenRemovalProcessQINYu12FANGFang2GUOJin-song21．SchoolofRiverandOceanEngineeringChongqingJiaotongUniversityChongqing400074China2．SchoolofUrbanConstructionandEnvironmentalEngineeringChongqingUniversityChongqing400045ChinaAbstractTheeffectsofpHonammoniaoxidizingbacteriaAOBnitriteoxidizingbacteriaNOBandanaerobicammoniumoxidizingANAMMOXbacteriaandontheoverallnitrogenremovalperformanceofSBBRwereinvestigated．SpecificPCRprimerswereusedtodeterminethepopulationoffunctionalbacteriaintheSBBRprocess．TheresultsindicatedthatthenitrogenremovalperformanceandthepopulationsofAOBNOBandANAMMOXbacteriaweregreatlyinfluencedbypH．ThepopulationsofallthreetypesofbacteriawerethelowestatpH6．0．AOBthrivedinthepHrangeof7．0to8．0butwasinhibitedatpH9．0．NosignificantchangeinNOBpopulationwasobservedinthepHrangeof7．0to9．0．ThepopulationsofallthebacteriawerethehighestatpH8．0．TheSBBRachievedmaximumni-trogenremovalperformancewhenoperatedatpH8．0．KeywordsSBBRautotrophicnitrogenremovalpHreal-timePCRAOBNOBANAMMOX·63·281720129CHINAWATER＆WASTEWATERVol．28No．17Sep．2012、。、。AOB、NOBANAMMOX312AOB、NOBANAMMOXDO、3～5。pHpH。SBBRPCRpHSBBR。11.1SBBR1。1Fig．1SchematicdiagramofSBBRreactor。NH4HCO3160mg/LFe、Mg。80%100%6。pH。1.2pHSBBRK717DNADNA。TaqDNAPCRpMD18－T7。1.3PCRSYBRGreenI。PCR1。PCR。95℃×1min55℃×1min55℃0．5℃10s8095℃。1PCRTab．1Primersandparametersofreal-timePCRAOBCTO189fA、B/RT1r95℃×5min95℃×15s60℃×45s458NOBNSR1113f/NSR1264r95℃×5min95℃×1min60℃×1min72℃×2min359ANAMM-OXAMX818F/AMX1066R50℃×2min94℃×10min94℃×15s60℃×1min401022.1GenBank。2。2GenBankTab．2PartialresultofrecombinantsequencealignmentwithgenesinGenBank/bpⅠ117FJ445022CP00045098%NitrosomonasⅡ151FJ490152AJ224046100%NitrospiraⅢ275FJ490144AF37599599%CandidatusKueneniastuttgartiensis16SrDNAAOB、NOBANA-MMOXPCRDNA。·73·．watergasheat．compHSBBR28172.2pHpH6．0～9．0。pH=8．0、7．0、9．0、6．0100d。1～21pH8．093%～100%NO－2－N75%～82%。22～51pH7．0。pHpH8．0。29NH+4－N60mg/LNO－2－N1～2mg/L20～30mg/L。NO－3－N30mg/L5mg/L。71%58%。52～83pH9．0NO－2－N818234～38mg/LNH+4－N100～130mg/L31%12%。84pH6．0150mg/L。2.3pHpHPCR3。pHAOB。pH6．0AOBpHAOB。pH7．0AOB8．25×10104．68×1010cells/g。pH8．0AOBpH1～4AOB。pH9．0FAFA52．73mg/L10mg/L11。pHNOBAOBpH6．0pH3～4pH8．0NOB。pH7．0～9．0NOBAOB。3pHTab．3BacterialpopulationunderdifferentpHcells·g－1AOBNOBANAMMOXpH=6．04．25×1071．38×1042．21×1073．66×1071．16×1042．07×107pH=7．08．25×10106．96×1074．50×10104．68×10105．71×1075．57×1010pH=8．02．75×10115．84×1084．90×10116．98×10103．23×1081．22×1012pH=9．04．94×1094．09×1073．02×1092．72×1092．46×1073．76×109ANAMMOX。pHANAMMOX107cells/g1012cells/gpH。pHANAMMOXNH3HNO2NH3NH+4HNO2NO－2pHNH3HNO2ANAMMOX。pH8．0ANAMMOX4．90×10111．22×1012cells/g。3SBBRAOB、NOBANAMMOXAOBNOBNH+4－NNO－2－NNO－3－NANAMMOXNH+4－NNO－3－NNO－2－NN2。。pHpHSBBR。pHpHFApH。pHAOB。·83·2817．watergasheat．comAOBpHpH7～8AOB。pHAOBAOBpHFA。JettenANAMMOXpH6．7～8．38．0。Egli12KueneniastuttgartiensispH6．5～9．08。13ANAMMOXpH7．5。。4pH6．0AOB、NOB、AN-AMMOXpH。pH8．0pHSBBRpH。1SliekersAODerwortNGomezJLCetal．Completelyautotrophicnitrogenremovalovernitriteinonesinglere-actorJ．WaterRes200236102475－2482．2ThirdKASliekersAOKuenenJGetal．TheCAN-ONsystemcompletelyautotrophicnitrogenremovalo-vernitriteunderammoniumlimitationinteractionandcompetitionbetweenthreegroupsofbacteriaJ．Sys-tematicandAppliedMicrobiology2001244588－596．3．DOJ．20072891975－1980．4．J．2009302191－196．5．SBRJ．2005252222－225．6．J．2010323113－118．7．J．2009466773－779．8．PCRJ．2007471136－140．9DionisiHMLaytonACHarmsGetal．QuantificationofNitrosomonasoligotropha-likeammonia-oxidizingbac-teriaandNitrospirasppfromfull-scalewastewatertreat-mentplantsbycompetitivePCRJ．ApplEnvironMi-crobiol2002681245－253．10TsushimaIKindaichiTOkabeS．Quantificationofan-aerobicammonium-oxidizingbacteriainenrichmentcul-turesbyreal-timePCRJ．WaterRes2007414785－794．11FuxCBoehlerMHuberPetal．Biologicaltreatmentofammonium-richwastewaterbypartialnitrificationandsubsequentanaerobicammoniumoxidationanammoxinapilotplantJ．JBiotechnol2002993295－306．12EgliKBosshardFWerlenCetal．Microbialcomposi-tionandstructureofarotatingbiologicalcontactorbio-filmtreatingammonium-richwastewaterwithoutorganiccarbonJ．MicrobiolEcol2003454419－432．13．J．2001172193－197．1981－。E－mailqinyu54001@163．com2012－03－22·93·．watergasheat．compHSBBR2817