PVC纤维棉上生物膜的形成及其硝化性能研究刘飞

2007,16(2):[e-mail]2004B202010251969E-mail:[e-mail]2006-11-061.4120002.510640PVC13~15d0.3mg·L-124~26d5~6dPVC4.8~7.2×105CFU·cm-32~2.3×105CFU·cm-36.4~8.6×104CFU·cm-3“”PVC“”PVC25269.4±4.82mg·m-2·h-1168.1±13.1mg·m-2·h-1PVCX703A1672-2175200702-0394-05MediaPVCPSPEPPPVCFiberfillPVC“”10~30d[1~3]PVCPVCPVC3cm30cm×10cm840cm20.8±0.1kg·m-3(3cm×3cm)121m386mg·L-1()pH20PVC1265%361MO554MO1MO2PO3-4-P0.01mg·L-1MO30.3mg·L-1MO40.6mg·L-1MO51.0mg·L-1(AP)pHTab.1TheexperimentregimeofbiofilmformationLALOMAMOHAHO161624242929300g16g+DOI:10.16258/j.cnki.1674-5906.2007.02.025395MAMO310“”2582(Totalammonianitrogen,TAN)5mg·L-12mg·L-12h3hRTANRnitriteRTAVC⋅⋅∆R(mg·m-2·h-1)CT(mg·L-1)V(L)T(h)A(m2)pHpH315iOxi315i(WTW,)(Hach®DR-890)Tetra®[4]3Tween-800.1mL200mL30minSPSSRe-lease10.0pH1abHOMOHAMALALO1aLALO18d4.95~5.91mg·L-10.0013~0.029mg·L-115“”“”HOMO1b6~7d15.9~18.1mg·L-16dMO1213~14d65%0.3~0.5mg·L-19~11d14~15d7.46~8.01mg·L-1MO1MO316d2“”13~15dTab2ChangesofbacteriapopulationdevelopingintheprocessofbiofilmformationonPVCfiberfillCFU·cm-3MAMO1MO3757.5×1066.8×10629591625425.4×1066.1×10625.2×1055.5×1051646415.1×1063.3×1061.5×1052.1×105267.7×102**1.6×105**814.8×1057.2×10532.3×1052×105308.6×1046.4×104**MO1MO3(P0.01)a03691215182124273033/d)/mg·L-1b0246810121416182003691215182124273033mg·L-1116dFig.1TimeprofilesofTANandnitriteintheprocessofbiofilmformationThearrowindicatedtheadditionofphosphateonday1639616220073HAMA18d4.55~5.02mg·L-1NO2N=0.06~0.027mg·L-1MA32-----“”“”[2,5][2,6]CNP16dMO7.2~8.01mg·L-11bMO1MO228~29d30d65%0.05mg·L-130dMO12“”30dMO3MO4MO523~24d24d65%0.03mg·L-126dMO32MO1(t-test;P=0.005)5~6d“”24~26d0.3mg·L-1“”0.002~0.05mg·L-1[7-9]MO1MO2“”“”[9][10]0.3mg·L-15~6d“”22Michealis-MentenMonod[1]2.4~5.04mg·L-1269.4±4.82mg·m-2·h-10.32~2.01mg·L-1168.1±13.1mg·m-2·h-1[11]0.3~0.55g·m-2·d-10.44g·m-2·d-1254.98g·m-2·d-13.24g·m-2·d-13“”0501001502002503000123456ρ()/(mg•L-1)/(mg•m-2•h-1)Fig.2TANornitriteremovalratesofthebiofilmsatthedifferentconcentrationsofTANornitrite397[12]3[12][13](2)[13]1PVC2“”“”13~15d“”“”30.3mg·L-1“”24~26d5~6d“”4PVC4.8~7.2×105CFU·cm-32~2.3×105CFU·cm-36.4~8.6×104CFU·cm-35PVC25269.4±4.82168.1±13.1mg·m-2·h-16“”PVC[1],,.[M].:,2000:128-158.LIUYu,ZHAOQingliang,ZHENGXincan.WastewaterTreatmentTechniquebyBiofilm[M].Beijing:ChemicalIndustryPress,2000:128-158.[2],,,.Cd2+[J].,2005,14(5):636-639.KANGChunli,GUOJing,GUOPing,etal.BiosorptionofCd2+bytheorganiccomponentsofsurfacecoatingsinnaturalwaters[J].Ecol-ogyandEnvironment,2005,14(5):636-639.[3]FONTENOTQ,BONVILLAINC,KILGENM,etal.Effectsoftem-perature,salinity,andcarbon:nitrogenratioonsequencingbatchreac-tortreatingshrimpaquaculturewastewater[J].BioresourceTechnology,2007,98(9):1700-1703.[4],,.[M].:,2001:199-201.XUYatong,SHIJialiang,ZHANGMing.MicrobiologicalEngineer-ingforControllingPollution[M].Beijing:ChemicalIndustryPress,2001:199-201.[5],,,.[J].,1999,7(5):43-51.WANGWenjun,WANGWenhua,HUANGYabing,etal.ProgressofBiofilmStudies[J].TechniquesandEquipmentforEnvironmentPollu-tionControl,1999,7(5):43-51.[6]STOODLEYLH,STOODLEYP.Developmentalregulationofmi-crobialbiofilms[J].CurrentOpinioninBiotechnology,2002,13:228-233.[7]BARAKY,VANRJ.Biologicalphosphateremovalinaprototyperecirculatingaquaculturetreatmentsystem[J].AquaculturalEngineer-ing,2000,22:121-136.[8]LIUF,HANWY.Reusestrategyofwastewaterinprawnnurserybymicrobialremediation[J].Aquaculture,2004,230:281-296.[9]DAUMERML,BÉLINEF,GUIZIOUF,etal.InfluenceofpHandbiologicalmetabolismondissolvedphosphorusduringbiologicaltreatmentofpiggerywastewater[J].BiosystemsEngineering,2007,96(3):379-386.[10]GUISASOLAA,PETZETS,BAEZAJA,etal.Inorganiccarbonlimitationsonnitrification:Experimentalassessmentandmodel-ling[J].WaterResearch,2007,41:277-286.[11]LEKANGOI,KLEPPEH.Efficiencyofnitrificationintricklingfiltersusingdifferentfiltermedia[J].AquaculturalEngineering,2000,21:181-199.[12]HENZEM,HARREMOESP,JANSEnJ,etal.Wastewatertreatment:BiologicalandChemicalProcesses[M].2nded.Berlin,Germany:Springer,1997:56.[13]TANJIY,SAKAIR,MIYANAGAK,etal.Estimationoftheself-purificationcapacityofbiofilmformedindomesticsewerpipes[J].BiochemicalEngineeringJournal,2006,31(1):96-101.01234560369121518212427303336/h()/(mg•L-1)()()Fig.3TimeprofileofTANandnitritelevelsinevaluatingbiofilmnitrification39816220073StudyonbiofilmformationandnitrificationperformanceofPVCfiberfillLIUFei1,2,LIXiaolong1,ZHAOQiangzhong2,ZHAOMouming21.KeyLabofGreenPackagingandbiologicalNanotechnology,HunanUniversityofTechnology,Zhuzhou412000,China;2.CollegeofLightindustryandFoodSciences,SouthchinaUniversityofTechnology,Guangzhou510640,ChinaAbstracts:TheinfluenceofseveralfactorsonbiofilmformationonPVC(Polyvinylchloride)fibrefillanditsnitrificationperform-ancewerestudiedwhenitwasusedasmediainthebiofilmtechnique.Intheprocessofformingbiofilm,theexistenceoforganicswasinfavorofestablishingNitrosomonasonthefiberfill.ThematuretimeofNitrosomonasonfiberfillwas13~15days.ThegivenlevelofphosphatewasadvantageoustodevelopNitrobacteronmedia.Whenthephosphatelevelsweremaintainedabove0.