SBAR中同步脱氮好氧颗粒污泥的菌种特性

2005,25(2)218~221ChinaEnvironmentalScienceSBAR1,1,1,1,1,2(1.,1160232.,116029),.,,.,,,,.X17A10006923(2005)02021804CharacteristicofthestrainofsimultaneousdenitrificationbacteriaaerobicgranulesludgeinSBAR.YANGFeng-lin1,WANGFang1,ZHANGXing-wen1,LIULI-fen1,QIAi-jiu1,GONGZheng2(1.SchoolofEnvironmentalandBiologicalScienceandTechnology,DalianUniversityofTechnology,Dalian116023,China2.DepartmentofBiology,LiaoningNormalUniversity,Dalian116029,China).ChinaEnvironmentalScience,2005,25(2)218~221AbstractAerobicgranulesludgewascultivatedadoptinginternal-circulatesequencingbatchairliftreactor;andbacteriastrainpossessingdenitrificationactioninaerobicgranulessludgewerepurifiedseparatelyusingbatchwttivationtechnique.Eachbacteriastrainwasdistinguished;throughcontrollingoxygenconditioninthecultivatingprocess,thedenitrationgrowthcharacteristicsandaerobicpropertyofeachbacteriastrainwereinspected.Aerobicgranulesludgepurifiedcultivationcouldobtaindifferentlycultivatednitration-aerobicdenitrified,facultativesubritrifierandnitrifier.Theseparatingobtaineddenitrifiercouldgrowunderaerobicdenitrationcondition;therefore,themicrobeinaerobicgranulesludgepossesseddiversityandstrongeradaptability,andembodieddefinitelythecomplexityofthesludgemicrostructure.Keywordsaerobicgranularsludgeinternalcirculatesequencingbatchairliftreactorsimultaneousdenitrificationdifferentlycultivatednitration-aerobicdenitrification,[13].,,,.,,,[4,5].(IC-SBAR),,.11.1,[6].1.2[7].20040629(2002120113);(20030141022)*,,[e-mail]2SBAR2191.2.1:NaNO21.0g,Na2CO31.0g;NaH2PO40.25g;MnSO4·4H2O0.01g;MgSO4·7H2O0.03g;K2HPO40.75g;16~20g;pH7.2.:0.5g;KNO32.0g;KH2PO40.5g;MgSO40.2g;pH7.0~7.2.:(NH4)2SO41g;CaCl21.5g;FeSO4·7H2O0.03g;K2HPO41.0g;NaCl0.3g;MgSO4·7H2O0.03g;16~20g;pH7.0~7.2.1000mL.1.2.220mL,12130min;50,,;37,.1.2.3,SBAR,,30min10mL.101,102,103104.0.2mL,.3.,30.,.,4,.1.2.4,,,.1.2.5,50µL,.22.1SBAR,1.1,,,.1SEMFig.1MorphologyofaerobicgranulessurfacebySEM(a)(×4000),(b)(×60)2.22.2.13,,1.13Table1Morphologicalcharacteristicof3nitrousbacteriaYXH-1,,,,YXH-2,,,,YXH-3,,,350µL,(TEM)(2).2,YXH-1YXH-3,,[8],YXH-1YXH-3,;YXH-2,,,YXH-2.(a)(b)220252TEM(×20000)Fig.2TEMphotoofnitrousbacteria(×20000)2.2.23,,30,.,3,,,CO2,3,,30,.,YXH-1YXH-2,2.YXH-1,;YXH-2,.YXH-3,.YXH-1YXH-3,,2.2.2.32,,2.22Table2Morphologicalcharacteristicof2nitricbacteriaXH-1,,,XH-2,,,,50µL,(SEM)(3).[8],XH-1,XH-2.3SEMFig.3SEMphotoofnitricbacteria2.2.42,,,.,XH-1XH-2,2.XH-1,;XH-2,.2.2.55(3).,(a)XH-1(×10000)(b)XH-2(×10000)(a)YXH-1(b)YXH-2(c)YXH-32SBAR221,.35Table3Morphologicalcharacteristicof5denitrifyingbacteriaFXH-1,FXH-2FXH-3,FXH-4,FXH-5,2.2.65,,,.,FXH-4,FXH-4,,,.,.,,.33.1SBAR,.3.2,,,,,.[1]QinLei,TayJH,LiuYu.Selectionpressureisadrivingforceofaerobicgranulationinsequencingbatchreactors[J].Proc.Biochem.,2004,39(5):579585.[2]JangAm,YoonYoung-hua,KimInS,etal.CharacterizationandevaluationofaerobicgranulesinSBR[J].J.Biotech.,2003,105(1/2):7182.[3]YangSF,LiuQS,TayJH,etal.Growthkineticsofaerobicgranulesdevelopedinsequencingbatchreactors[J].Lett.Appl.Microbio.,2004,38(2):106113.[4],.[J].,2003,23(4):380384.[5]LiuYu,YangShu-Fang,TayJoo-Hwa.Improvestabilityofaerobicgranulesbyselectingslowgrowingnitrifyingbacteria[J].J.Biotech.,2004,108(2):161169.[6],,,.SBAR[J].,2004,24(6):725729.[7],.[M].:,2002.[8]RE,NE.[M].8..:,1984.(1944),,,,.70.,,,.,.,.⋅1960~1989,.,,2,.20050318