20119275JournalofShenyangJianzhuUniversityNaturalScienceSep.2011Vol.27No.52011-02-282009-k7-92010-k7-21960—.2095-1922201105-0952-05SBBR11121.1101682.110142SBBR.SBR/NO-3.32#、3#5#75.82%、78.26%62.48%.SBBR.X703AStudyonSeparationandIdentificationofDenitrifyingPhosphorus-RemovingBacteriainSBBRLIYafeng1HAOYing1ZHANGXiaoning1JINYanqiu21.SchoolofMunicipalandEnvironmentalEngineeringShenyangJianzhuUniversityShenyangChina1101682.SchoolofEnvironmentalandBiologicalEngineeringShenyangHuagongUniversityShenyangChina110142AbstractThestudyaimedtoprovetheabundantexistenceofphosphorus-accumulatinganddenitrifyingstrainsandtoinvestigatetheirdenitrifyingandphosphorusremovingperformancesinSBBRsystem.AddingpolyurethanecarriersinSBRtheexperimentusednitrateaselectronicreceptorstostudydenitrifyingphos-phatationunderanaerobic/anoxicconditions.Theactivatedsludgewasextractedinstabilizationcondition.Theexperimentuseddilutioncoatingmethodandflatcrossedmethodtoseparatethedenitrifyingphosphorusremovingbacteriaandalsousedmetachromaticgranulesdyeingmethodandnitratereducingtesttoidentifythescreeningbacteria.Theresultsshowedthatthreestrainswereextractedwhichhadbothdenitrificationandphosphorusremovingefficacy.TheyallhadmetachromaticgranulesandtheGram'sdyeingwasallneg-ative.Thenitrateremovalratesof2#3#and5#strainswererespectively75.82%78.26%and62.48%.LargeamountofphosphorusremovingbacteriaexistintheSBBRsystemandtheyperformgooddenitrifyingandphosphorusremovingeffect.Keywordsdenitrifyingphosphorusremovingbacteriaflatcrossedsequencingbacthbiofilmreactormeta-chromaticgranulesdyeingnitratereduction27SBBR9531.Kuba21994———DPBDenitrifyingPhosphorusremo-vingBacteriaPAOsO2NO-XPHB3.COD4-7CODCO2CO28.、9-10.1112.SBBR1314.SBBR..、SBBR15.11.11.1.1①、、、、②、③、④、、、.1.1.2①20g20g2g2g0.5g1000mLpH7.2~7.4.②NO-21g2.42g1g1g20g1000mL.1.1.3①0.15g0.2g1mL95%2mL100mL2g3g300mL.②95%14.1.2SBBRSBBR16....22.1SBBR10mL250mL90mL10-120min.10-11mL9mL10-29542710-21mL9mL10-52100.5mL40℃..10、30℃3d、.510-410-1、10-210-310-510-410-461.110-4Table1Growthcharacteristicofthecolonyindiluentplatingmedium1#、、、2#、、、3#、、、4#、、5#、、、6#、、2.2Albert142.2.1ATP....31.12#、3#5#Fig.1Metachromaticgranulesdyeingresultof2#3#and5#strainsPoly-P2#、3#5#.2.2.23.2#、3#5#...322.27SBBR95522#、3#5#Fig.2Gramstaindyeingresultsof2#3#and5#strains22#、3#、5#Table2Dyeingresultsof2#3#and5#strains2#3#5#2.31~7d17.8mL20min.30℃18.7d.31#、2#3#2#、3#5#.32#、3#5#Fig.3Nitratereducingtestresultsof2#3#and5#strains33.3.2#、3#5#333、57d123.3Table3Removaleffectofnitratereducingtest/%3d5d7d2#49.1567.8375.823#55.7170.5078.265#30.6246.6162.4831SBBR6.22#、3#5#.3.7d2#75.82%3#78.26%5#62.48%.1.J.2009253535-540.JiaoZhongzhiLiXiangkunZhangLichengetal.IsolationandcharacteristicofdenitrifyingphosphorusaccumulatingorganismJ.JournalofShenyang95627JianzhuUniversityNaturalScience2009253535-5402KubaTVanLoosdrechtMCMHerjnenJJ.Effectofcyclicoxygenexposureontheactivityofdenitrif-yingphosphorusremovingbacteriaJ.WatSciTech19963433-40.3.SBRJ.200840121927-1931.ShiYu-mingMaFangWangQiangetal.Isola-tionofPAOsandefficiencyofphosphorousaccumu-lationinSBRsystemJ.JournalofHarbinInstituteofTechnology200840121927-19314KubaTVanLoosdrechtMCMHeijnenJJ.Phos-phorusandnitrogenremovalwithminimalCODre-quirementbyintegrationofdenitrifyingdephosphata-tionandnitrificationinatwo-sludgesystemJ.Wat.Res.19963071702-1710.5KubaTSmoldersGVanLoosdrechtMCMetal.Biologicalphosphorusremovalfromwastewaterbyanaerobic-anoxicsequencingbatchreactorJ.Wat.Sci.Tech.1993275/6241-252.6MerzoukiMBernetNDelgenesJPetal.Biologi-caldenitrifyingphosphorusremovalinSBReffectofaddednitrateconcentrationandsludgeretentiontimeJ.Wat.Sci.Tech.2001433191-194.7Kerrn-JespersenJPHenzeM.BiologicalphosphorusuptakeunderanoxicandaerobicconditionJ.Wat.Res.1993274617-624.8.J.2007283517-521.DaiWenchenZhangHanminXiaoJingnietal.EnrichmentofdenitrifyingphosphateaccumulatingorganismsinsequencingbatchmembranebioreactorsJ.EnvironmentalScience2007283517-5219.J.199824368-73.HaoXiaodi.Europeanwaterenvironmentcontrolphosphorusstrategyandwastewaterdephosphoriza-tiontechnologyUpJ.Waterandwastewateren-gineering199824368-7310.D.2008.ChenLei.StudyonisolationandcharacteristicofdenitrifyingphophateaccumulatingbacteriaD.JiNanSchoolofenvironmentalscienceandengineer-inginShandonguniversity2008.11.J.2007286631-635.MaFangWangChunliWangLili.Screeningofdenitrifyingpolyphosphate-accumulatingorganismsandtheirbiologicalcharacteristicsJ.JournalofHarbinEngineeringUniversity2007286631-63512OehmenALemosPCCarvalhoGetal.Advancedinenhancedbiologicalphosphorusremovalfrommi-crotomacroscaleJ.WaterResearch2007412271-2300.13.SBBRJ.20115138-42.LiYafengYaoJingboHaoYingetal.Studyofaddingpolyurethanefoamasmicro-organismsimmo-bilizedcarriersinsequencingbatchbiofilmreactortoremovenitrogenandphosphorusJ.ChineseJournalofEnvironmentalEngineering20115138-4214.M.1990.QiuWenfang.Environmentalmicrobiologytechni-calmanualM.BeijingAcademypress1990.15TsunedaSOhnoTSoejimaKetal.Simultaneousnitrogenandphosphorusremovalusingdenitrifyingphosphate-accumulatingorganismsinasequencingbatchreactorJ.Biochemicaleng