SBR短程脱氮系统中亚硝酸盐积累对生物除磷的影响曾薇

31220112　　　　　　ActaScientiaeCircumstantiaeVol.31,No.2Feb.,2011:(No.50878005);(No.8102005);(No.121076)SupportedbytheNationalNaturalScienceFoundationofChina(No.50878005),theBeijingNaturalScienceFoundation(No.8102005)andtheFokYingDongEducationFoundation(No.121076):(1974—),,E-mail:[e-mail];＊(),E-mail:[e-mail]:ZENGWei(1974—),female,E-mail:[e-mail];＊Correspondingauthor,E-mail:[e-mail],,,.2011.SBR[J].,31(2):236-244ZengW,YangYY,LiL,etal.2011.Effectofnitritefromshort-cutnitrificationonbiologicalphosphorusremovalinanSBRprocesstreatingdomesticwastewater[J].ActaScientiaeCircumstantiae,31(2):236-244SBR曾薇,杨莹莹,李磊,王向东,彭永臻＊北京工业大学,环境与能源工程学院,环境工程研究所,北京100124:2010-05-11　　　:2010-06-29　　　:2010-07-02:SBR,DO,,.,,NO-2-N10mg·L-1,,1mg·L-1;NO-2-N20mg·L-1,5mg·L-1,,.(COD200mg·L-1),20d.,25～30mg·L-1.,,(PHA),,.,,30mg·L-1;,30mg·L-1PHA,,PHA.:;;;;:0253-2468(2011)02-236-09　　　:X703.1　　　:AEffectofnitritefromshort-cutnitrificationonbiologicalphosphorusremovalinanSBRprocesstreatingdomesticwastewaterZENGWei,YANGYingying,LILei,WANGXiangdong,PENGYongzhen＊DepartmentofEnvironmentalEngineering,CollegeofEnvironmentalandEnergyEngineering,BeijingUniversityofTechnology,Beijing100124Received11May2010;　　　receivedinrevisedform29June2010;　　　accepted2July2010Abstract:Alab-scaleanaerobic/aerobicsequencingbatchreactor(SBR)wasoperatedtoinvestigateitsperformanceinbiologicalnutrientremovalfromdomesticwastewater,particularlyregardingtheeffectofnitriteaccumulationresultingfromshort-cutnitrificationonbiologicalphosphorusremoval(BPR).Short-cutnitrificationwassuccessfullyachievedbycontrollinglowDOconcentration(0.5～1.0mg·L-1)andaerobicduration.Theresultsshowedthatlessthan10mg·L-1ofnitritehadnoeffectonphosphateuptakeandreleasewithPO3-4-Pconcentrationsintheaerobiceffluentofbelow1mg·L-1;whereasasignificantdecreaseofphosphateuptakeandreleasewiththeeffluentPO3-4-Pconcentrationof5mg·L-1wasobservedwhennitriteaccumulationreached20mg·L-1,indicatingdeteriorationofBPR.Afteraddingexternalcarbonsources(initialCODconcentrationof200mg·L-1),BPRperformancerecoveredafter20days.Theresultssuggestthatthe25～30mg·L-1ofnitriteaccumulatedduringtheaerationperioddidnotinhibitaerobicphosphateuptake.Themainreasonforpoorphosphorusremovalwasthatthepresenceofnitriteintheanaerobicperiodcauseddenitrifyingbacteriatocompetewithphosphateaccumulatingorganisms(PAOs)forlimitedcarbonsources.Loweredpoly-β-hydroxyalkanoate(PHA)synthesisinPAOsledtodecreasedphosphateuptakeintheaerobicstage.BatchexperimentswereperformedtoinvestigatetheeffectofnitriteonPAOsunderanaerobicconditions,whichindicatedthatnitriteasaninhibitorataconcentrationof30mg·L-1wouldnotinhibittheanaerobicphosphaterelease.Whensufficientcarbonsourcewasprovided,nitriteof30mg·L-1hadnoimpactonPHAstorage;contrarily,whenthecarbonsourcewasinsufficient,denitrifyingDOI:10.13671/j.hjkxxb.2011.02.0042:SBRbacteriacompetingforcarbonsourcewithPAOsresultedinadecreaseofPHAsynthesisandphosphaterelease.Keywords:nitrite;short-cutnitrification;phosphateaccumulatingorganisms(PAOs);biologicalphosphorusremoval(BPR);domesticwastewater1　(Introduction)、,.,(Bernetetal.,2005;Pengetal.,2004).,,(vanHulleetal.,2007;Vadiveluetal.,2006).,,,,.(Enhancedbiologicalphosphorusremoval,EBPR),,(Phosphateaccumulationorganisms,PAOs),(Volatilefattyacid,VFA),PHA;,PAOsPHA,,(Oehmenetal.,2007).,EBPR,Meinhold(1999)EBPR,,NO-2-N8mg·L-1.Saito(2004)//SBRPAOs,PAOs,PAOs.,(Yoshidaetal.,2004;Zhouetal.,2004).,EBPR.,SBR,,,,.2　(Materialsandmethods)2.1　试验装置及污泥,.2SBR.SBR1:,11L,1/2,/,3,180d.,1.SBR2:,7L,1/2,/,3,7h(2h,4h,1h)..1　SBR1Table1　SBR1operationforpartialnitrificationtonitrite/d/h/h/h-/h/(mg·L-1)0～3023110.5～1.031～6524110.5～1.065～18025110.5～1.02.2　试验用水,.SBR,:COD194.5mg·L-1,NH+4-N69.44mg·L-1,PO3-4-P5.71mg·L-1.,(1L):180mgMgSO4·7H2O,21mgCaCl2·2H2O,3mg,0.6mL.,(1L):1.5gFeCl3·6H2O,0.15gH3BO3,0.03gCuSO4·5H2O,0.18gKI,0.12gMnCl2·4H2O,0.06gNa2MoO4·2H2O,0.12gZnSO4·7H2O,0.15gCoCl2·6H2O,10gEDTA.237　　　　　　　　　　312.3　静态试验SBR24L,341.5LSBR,1.5L,1.5LSBR0.45g1.8g,COD200mg·L-1800mg·L-1.,,40、10、2030mg·L-1.pH,0.5mol·L-1HCl0.5mol·L-1NaOHpH,pH=7.50±0.05,(25.0±0.5)℃.SBR23.2.4　分析测试方法(COD、NH+4-N、NO-2-N、PO3-4-P);PHA(Brauneggetal.,1978)((PHB)(PHV))(Chenetal.,2005),6890N;pHWTWMultiLine340i.(FreeNitrousAcid,FNA)pH,(1)(Anthonisenetal.1976).FNA=[NO-2-N]Ka×10pH(1),Ka,Ka=e-2300/(273+T),,T(℃).3　(Results)3.1　亚硝酸盐积累对生活污水生物除磷系统的影响SBR1/180d,,4.1:1～65d;2:66～92d;3:93～113d;4:114～180d.1、.1,1,0.72mg·L-19.55mg·L-1,,1mg·L-1.,.2,,,26.56%57.55%,,9.55mg·L-120mg·L-1,95.,5mg·L-1.3,1.6g,COD200mg·L-1.20mg·L-1,10mg·L-1,109d,6.9mg·L-1.41　NO-2-NPO3-4-PNH+4-NFig.1　NO-2-N、PO3-4-PattheendofaerationphaseandNH+4-NremovalefficiencyinSBR12382:SBR97%,20mg·L-1,2.27mg·L-1,1mg·L-1.,10mg·L-1,,,(2008).2.2,2,.3,COD200mg·L-1,.4,,1,29.21mg·L-132.08mg·L-1,,.VFA,PHA.2,VFA,PHA.2　Fig.2　AnaerobicphosphorusreleaseandaerobicphosphorusuptakeinSBR13.2　亚硝酸盐对聚磷菌厌氧代谢的影响3SBR1.3,,,.4,,12.,,.,2.①;②,VFAPHA.:1200mg·L-1;2800mg·L-1,,.2SBR2,,99%.SBR1SBR2,.3.2.1　碳源不足情况下亚硝酸盐对聚磷菌厌氧代谢的影响　41SBR(HAc)、、PHA,120min.4a,10、2030mg·L-1,90、5050min,.4b4c120minPHA.4b,,30mg·L-150min239