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3712Vol．37．No．12201812JOUＲNALOFXUCHANGUNIVEＲSITYDec．20182017－10－19PTZD20170111985—．1671－9824201812－0048－06SBＲC60230601SBＲC60．C60、、、、6hC6095．5%67．7%66．7%C60、PCＲ－DGGEC60．C60OUＲTOCX592A1－45．6．789、1011、12－14．C6015C6016．．C60SBＲPCＲ－DGGEC60．11．1C60、、、、、、、．．．COD=500mg/LNH4Cl95mg/LK2HPO4·3H2O30mg/LCaCl225mg/LMgSO420mg/LC∶N∶P=100∶5∶1．1．21．2．1C6020．70mgC6050mL100mL．10．00mL100mL．2．00、4．00、6．00、8．00、10．00mLC6025mL20．00mL．3712SBＲC60330nm．C60．1．2．2C604mgC60250mL24h0．45μm．300mL200mLC60100mL．UV－VisC60330nmC60．1．2．3C602．5L4SBＲ．24h、MLSS2000mg/L．1、2200mL48．27mg/LC603、4．0．4m3/hSBＲ20℃6hOUＲ．DNAPCＲ－DGGE．1．2．4PCＲDGGEDNADNA．F3385＇－ACTCCTACGGGAGGCAGCAG－3＇Ｒ5345＇－ATTACCGCGGCTGCTGG－3＇F3385＇40bpGC5＇－CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG－3＇16SrＲNAV3．PCＲ1%．DGGE8%30%～50%75V60℃D－CodesystemBioＲadLaboratoriesHer-culesCA14h1μg/mL20minGelDoc2000．1．2．5MLSS、VSS、SVI、NH3－N、OUＲ17TOCvarioTOCCUBEElementarζZetaNano－ZS．22．1C60C60nC60“”1819．C60UV－vis1UV－visC60．2C60100nm．UV－visnC60218、274、343nmC60400～500nmC60πnC60－20nC60．C60ζ－31．64±1．35mV．nC60nC6021．C60nC60－13．5～－50mV22．9．942018121C60UV－Vis2C602．2C60OUＲ．OUＲOUＲ23．3C60C60OUＲ．．OUＲ160mg/L/h40minOUＲ．10mg/L/h．．OUＲC60．2．3C60．410mg/L．．90min0．45mg/L95．5%．390min．．C60．34NH3－N053712SBＲC602．4C60．5TOC40min1．85mg/L/min67．7%66．7%．53、45OUＲTOC．．OUＲTOCOUＲOUＲTOC．TOC40minOUＲTOC67%OUＲ．90minOUＲ．2．5C60PCＲ－DGGE、PCＲ、24、25、26．、6hDNADNAPCＲDGGE．PCＲ－DGGE．6DGGEABCnC60．6SBＲDGGE．．3～4．a、b、c、d．C60C60．C60．Nyberg27C6090C60．Tong16C60．C60．C60．．1520181231C60SBＲ．C6090minNH3－NTOC95．5%66．7%C6095．5%67．7%．2PCＲ－DGGE、C60．C60．1ＲOBEＲTFS．NanomaterialsshowsignsoftoxicityJ．Science200330011243．2GEOFFB．NanotechnologyAlittleknowledgeJ．Nature200342417246－248．3ＲOBEＲTFS．NanotehcnologycallsriseformoreresearchontoxicologyofnanomaterialsJ．Science200531091609．4COLVINVL．ThepotentialenvironmentalimpactofengineerednanomaterialsJ．NatureBiotechnology200321101166－1170．5．J．20173611－15．6．－J．20161083977－3985．7HESSDJ．EnvironmentalreformorganizationsandundonescienceintheUnitedStatesexploringtheenvironmentalhealthandsafetyimplicationsofnanotechnologyJ．ScienceasCulture2010192181－214．8SAYESCMFOＲTNEＲJDGUOWetal．Thedifferentialcytotoxicityofwater－solublefullerenesJ．NanoLetters20044101881－1887．9SAYESCMGOBINAMAUSMANKDetal．Nano－C60cytotoxicityisduetolipidperoxidationJ．Biomaterials200526367587－7595．10LYONDYADAMSLKFALKNEＲJCetal．AntibacterialactivityoffullerenewatersuspensionseffectsofpreparationmethodandparticlesizeJ．Environ．Sci．Technol．200640144360－4366．11KANGSMAUTEＲMSELIMELECHM．Microbialcytotoxicityofcarbon－basednanomaterialsimplicationsforriverwaterandwastewatereffluentJ．Environ．Sci．Technol．2009432648－2653．12FANWLIUYXUZetal．ThemechanismofchronictoxicitytoDaphniamagnainducedbygraphenesuspendedinawatercolumnJ．EnvironmentalScienceNano201631405－1415．13OBEＲDＲSTEＲE．ManufacturednanomaterialsfullereneC60induceoxidativestressinthebrainofjuvenilelargemouthbassJ．Environ．HealthPersp．2004112101058－1062．14OBEＲDＲSTEＲEZHUSQBLICKLEYTMetal．Ecotoxicologyofcarbon－basedengineerednanoparticleseffectsoffullereneC60onaquaticorganismsJ．Carbon20064461112－1120．15JOHANSENAPEDEＲSENALJENSENKAetal．EffectsofC60fullerenenanoparticlesonsoilbacteriaandprotozoansJ．Environ．Toxicol．Chem．2008271895－1903．16TONGZHBISCHOFFMNIESLetal．ImpactoffullereneC60onasoilmicrobialcommunityJ．Environ．Sci．Technol．2007412985－2991．17．M．4．2002．18MCHEDLOV－PETＲOSSYANNOKLOCHKOVVKANDＲIEVSKYGV．ColloidaldispersionsoffullereneC60inwa-terSomepropertiesandregularitiesofcoagulationbyelectrolytesJ．J．Chem．Soc．FaradayTrans1997934343－4346．19DEGUCHISALAＲGOVAＲG．TSUJIIK．StabledispersionsoffullerenesC60andC70inwaterJ．Preperationandcharacterization．Langmuir2001176013－6017．253712SBＲC6020ANDＲIEVSKYGVKLOCHKOVVKBOＲDYUHABetal．Comparativeanalysisoftwoaqueous－colloidalsolutionsofC60fullerenewithhelpofFTIＲreflectanceandUV－VisspectroscopyJ．ChemicalPhysicsLetters20023648－17．21LUDWIGＲAPPELHAGENA．Calculationofclathrate－likewaterclustersincludingH2O－buckminsterfullereneJ．Angew．Chem．Int．Ed．200544811－815．22DHAWANATAUＲOZZIJSTAＲABAＲAVVetal．StablecolloidaldispersionsofC60fullerenesinwaterevidenceforgenotoxicityJ．Environ．Sci．Technol．200640237394－7401．23SUESCUNJIＲIZAＲIOSTOLAZAXetal．DissolvedoxygencontrolandsimultaneousestimationofoxygenuptakerateinactivatedsludgeplantsJ．WaterEnviron．Ｒes．1998703316－322．24GＲIFFITHSＲIWHITELEYASO＇DONNELLAGetal．InfluenceofdepthandsamplingtimeonbacterialcommunitystructureinanuplandgrasslandsoilJ．FEMSMicrobiologyEcology20034335－43．25NICOLAISENMHＲAMSINGNB．DenaturinggradientgelelectrophoresisDGGEapproachestostudythediversityofammonia－oxidizingbacteriaJ．JournalofMicrobiologicalMethods200250189－203．26YAOYLGUANJTANGPetal．AssessmentoftoxicityoftetrahydrofuranonthemicrobialcommunityinactivatedsludgeJ．BioresourceTechnology20101015213－5221．27NYBEＲGLTUＲCOＲFNIESL．AssessingtheimpactofnanomaterialsonanaerobicmicrobialcommunitiesJ．Envi-ron．Sci．Technol．2008421938－1947．EffectsofC60ontheActivatedSludgeProcessinSBＲＲeactorsGUOHongxiaDean’sOfficeAnhuiSanlianUniversityHefei230601ChinaAbstractTheeffectsofshort－termtreatmentoffullereneC60aqueoussuspensiononbiochemicalcharacte