3912201312JOURNALOFBEIJINGUNIVERSITYOFTECHNOLOGYVol.39No.12Dec.2013SBR100124sequencingbatchreactorSBR./anoxic/oxicA/OdissolvedoxygenDOρDO0.5mg/LH.hydrossis.SBRA/OF/M0.83kg/kg·dρDO2.0mg/LsludgevolumeindexSVI300mL/g.fluorescenceinsituhybridizationFISHH.hydrossistotalnitrogenTN31.17%COD65.04%.F/M0.37kg/kg·dρDO2.0mg/LSVI150mL/g.NH+4-N100%ρNH+4-N0TNCOD70.7%73.9%.ρTNρCOD18.446.9mg/LA.SBRF/MH.hydrossisFISHX703.1A0254-0037201312-1880-072012-12-18.51108005.1973—E-mailgaochundi@bjut.edu.cn.FilamentousSludgeBulkingCauseAnalysisUnderLowDissolvedOxygenandControlMethodinSBRProcessGAOChun-diJIAOEr-longLIHaoWANGWei-xiaoPENGYong-zhenCollegeofEnvironmentalandEnergyEngineeringBeijingUniversityofTechnologyBeijing100124ChinaAbstractBytreatingrealdomesticsewagewhichislowρC/ρNinsequencingbatchreactorSBRthecauseoffilamentoussludgebulkingandtheeffectofcontrollingfilamentoussludgebulkingunderlowdissolvedoxygenwithdifferentoperationpatternswereinvestigated.ResultsshowedthatwhenthedissolvedoxygenDOvaluewas0.5mg/Linanoxic/oxicprocessA/OfilamentoussludgebulkinghappenedandH.hydrossiswasthedominantfilamentousbacteriainfilamentousbulkingsludge.DuringA/OpatterninSBRsludgevolumeindexSVIhasmaintainedbeyond300mL/gwhenorganicloadingF/Mwas0.83kg/kg·dandtheρDOwasabout2mg/Lthesludgebulkingcouldnotbeeffectivelycontrolled.TheresultoffluorescenceinsituhybridizationFISHmanifestedthatthemainfilamentousbacteriawasalsoH.hydrossisfilamentousbacteria.InthisperiodaverageremovalratesoftotalnitrogenTNandCODwere31.17%and65.04%respectively.ThenduringaerobicpatternwhenF/Mwas0.37kg/kg·dtheρDOwasalsoabout2mg/LthesludgebulkinghasbeencontrolledeffectivelyandSVIhasremainedbelow150mL/g.InthisstagetheremovalrateofNH+4-Nreached100%nearlytheρNH+4-Nineffluentwasalmost0.AverageremovalratesofTNandCODimprovedto70.7%and73.9%respectively.TheaverageρTNandρCODineffluentwere18.412SBRand46.9mg/LrespectivelyclosetothenationallevelAemissionstandard.KeywordssequencingbatchreactorSBRorganicloadingsF/MlowdissolvedoxygensludgebulkingH.hydrossisfilamentousbacteriafluorescenceinsituhybridizationFISH..1-4.2.5.6.7-9..sequencingbatchreactorSBRSBRSBR.10-11.SBRH.hydrossisfluorescenceinsituhybridizationFISH.11.1SBR1SBR8L.1/anoxic/oxicA/O250%F/M.、、、3dissolvedoxygenDOρDO.mixedliquidsuspendedsolidsMLSSρMLSS1900~3500mg/L23℃.A/O.1SBRFig.1SchematicdiagramoftheSBR1.21.1.3COD、PO3-4-P、NH+4-N、NO-3-N、NO-2-N、MLSS、suspendedsolidSS、sludgevolumeindexSVItotalnitrogenTNmultiN/C3000AnalytikJenaAG.DOWTWMulti340i.OLYMPUS2BX61S-4300.188120131Table1WastewatercharacteristicpHρCOD/mg·L-1ρNH+4-N/mg·L-1ρNO-3-N/mg·L-1ρNO-2-N/mg·L-1ρPO3-4-P/mg·L-17.0~7.8121.8~307.243.6~80.40.02~1.290.03~0.563.6~6.922.1A/OF/M0.28kg/kg·d100%150%1∶1.753.04hρDO0.5mg/LSVI500mL/g.SVI2.2SVIFig.2VariationofSVIduringmalignantbulkingunderlowdissolvedoxygenFISHMPA60、MPA223MPA645M.parvicella12HHYH.hydrossis13.H.hydrossis3.H.hydrossis141F/M0.2kg/kg·d2345200μm0.3~0.4μm.A/O.F/M0.28kg/kg·d、.ρDO0.5mg/L.H.hydrossis.3H.hydrossisFISH1000Fig.3PictureofH.hydrossisinmalignantbulkingsludgeunderlowdissolvedoxygenbyFISH1000times2.2A/O1A/O→2h→2h→、1hF/M0.83kg/kg·d50%.23℃ρDO2.0mg/L.SVI4.448SVIA/O300mL/gSVI373mL/g.FISHHHYH.hydrossis5.288112SBR4A/OSVIFig.4VariationofSVIduringA/OpatternH.hydrossis.A/OSVI.5A/OH.hydrossisFISH1000Fig.5PictureofH.hydrossisinbulkingsludgeduringA/OpatternbyFISH1000times1COD6、7.1A/OSBRNH+4-N54.94%TN31.17%COD65.04%.6A/OFig.6VariationofnitrogenremovalduringA/Opattern7A/OCODFig.7VariationofCODremovalduringA/Opattern95%15、SS16.NH+4-NTN.COD17.A/OSVI300mL/g.388120132.3.0.25~0.45kg/kg·dSVI18.Chao190.6~1.3kg/kg·d1.8kg/kg·d1F/M0.83kg/kg·d.2→2.5h→、1hF/M0.37kg/kg·d50%.23℃ρDO2.0mg/L.SVI8.8SVIFig.8VariationofSVIduringaerobic-pattern8SVI35SVI150mL/gSVI150mL/gSVI122.6mL/g.COD9、10.NH+4-N100%NH+4-NTN70.7%39.53%COD73.9%8.86%.M.Beccari20.TN.ρDO9Fig.9Variationofnitrogenremovalduringaerobic-pattern10CODFig.10VariationofCODremovalduringaerobic-patternρDO21-2223-2415N25-26.ρTN20mg/L18.4mg/LCOD50mg/L46.9mg/LρNH+4-N0A.ρDOρDO27-29..021N、30.F/M0.37kg/kg·d488112SBR....31A/O0.28kg/kg·d100%150%HRT3.04hρDO0.5mg/LSVI500mL/gH.hydrossis.2SBRF/M0.83kg/kg·dA/O→2h→2h→、1hρDO2.0mg/LH.hydrossisSVI300mL/g.FISHH.hydrossis.TNCOD31.17%65.04%.32F/M0.37kg/kg·d→2.5h→、1hSVISVI150mL/g.NH+4-N100%TNCOD70.7%73.9%.ρNH+4-N0ρTNρCOD18.446.9mg/LA.1NOVKLLARREALWANNERJetal.Non-filamentousactivatedsludgebulkinginalaboratoryscalesystemJ.WaterResearch19932781339-1346.2GUOFengZHANGTong.ProfilingbulkingandfoamingbacteriainactivatedsludgebyhighthroughputsequencingJ.WaterResearch20124682772-2782.3.J.201127171-5.LIUXu-liangPENGYong-zhenPENGZhao-xuetal.ViscousactivatedsludgebulkingcausedbyhighaerationrateJ.ChinaWater&Wastewater201127171-5.inChinese4WENQin-xueCHENZhi-qiangWANGChang-yongetal.BulkingsludgeforPHAproductionenergysavingandcomparativestoragecapacitywithwell-settledsludgeJ.JournalofEnvironmentalSciences201224101744-1752.5.J.2012244129-132.ZHANGQiang.InthebiochemicalsewagetreatmentexpandresentmentandcontrolmeasureresearchJ.NorthernEnvironment2012244129-132.inChinese6.A2/OJ.2008242333-35.WUChang-yongPEN