SBR生物反应器出水溶解态有机物性质特点研究郭瑾

31120111　　　　　　ActaScientiaeCircumstantiaeVol.31,No.1Jan.,2011:(No.50808003);(No.X0005013200801)SupportedbytheNationalNaturalScienceFoundationofChina(No.50808003)andtheDoctoralStart-upFundsofferedbyBeijingUniversityofTechnology(No.X0005013200801):(1979—),,(),E-mail:[e-mail];＊(),E-mail:[e-mail]:GUOJin(1979—),female,associatedprofessor(Ph.D.),E-mail:[e-mail];＊Correspondingauthor,E-mail:[e-mail],,,.2011.SBR[J].,31(1):20-25GuoJ,ZhangJR,ShangHL,etal.2011.CharacterizationofthedissolvedorganicmatterinthesewageeffluentofanSBR[J].ActaScientiaeCircumstantiae,31(1):20-25SBR郭瑾,张静蓉,尚会来,唐旭光,彭永臻＊北京工业大学环境与能源工程学院,北京100124:2010-04-19　　　:2010-07-18　　　:2010-08-09:SBR,,SBR.,,650Da、1700Da2000Da,(200Da)(500～3000Da)(10000Da);SBR,SBR650Da20000Da,.:;;;:0253-2468(2011)01-20-06　　　:X703.1　　　:ACharacterizationofthedissolvedorganicmatterinthesewageeffluentofanSBR　GUOJin,ZHANGJingrong,SHANGHuilai,TANGXuguang,PENGYongzhen＊SchoolofEnvironmentalandEnergyEngineering,BeijingUniversityofTechnology,Beijing100124Received19April2010;　　　receivedinrevisedform18July2010;　　　accepted9August2010Abstract:ThetransformationofdissolvedorganicmatterinsequencingbatchreactorswasinvestigatedbytreatingdomesticwastewaterinanSBRwithdifferentoperationalparameters.Theimpactofsludgeretentiontime(SRT)wasmainlyanalyzedwithrespecttothecharacterizationofeffluentorganicmatter(EfOM)insewageeffluentfromtheSBR.Theresultsrevealedthathumic-likesubstanceswithmolecularweight(MW)of650Da,1700Daand2000Dawereaccumulatedduringthebiologicaldegradationprocess.MediumMW(500～3000Da,humicsubstances)andlargeMWorganicmaterials(10000Da,SMPs)weretransformedintolowMWorganicmaterials.ThemolecularweightdistributionofEfOMinsewageeffluentwasrarelyimpactedbySRT,onlyanintensitydifferenceinMWof650Daand20,000Dawasobserved,whichmeansthatalongerSRTwashelpfulforthedegradationofhumic-likesubstancesandsolublemicrobialproducts(SMPs).Keywords:sludgeretentiontime;effluentorganicmatter;molecularweightdistribution;solublemicrobialproducts1　(Introduction)(Biologicallytreatedsewageeffluent,BTSE)(Effluentorganicmatter,EfOM).:①(Naturalorganicmatter,NOM);②;③(Solublemicrobialproducts,SMPs)(Shonetal.,2006a;JarusutthirakandAmy,2006;BarkerandStuckey,1999).EfOM,(JarusutthirakandAmy,2006):COD、BOD,、、;,;DOI:10.13671/j.hjkxxb.2011.01.0121:SBR;、(JarusutthirakandAmy,2006;Rosenbergeretal.,2006;Shonetal.,2006b;Parketal.,2006;Jarusutthiraketal.,2002);(SirivedhinandGray,2005;Chenetal.,2009).、,EfOM,,、、、pH(GuoandPeng,2008),(AquinoandStuckey,2004;KuoandParkin,1996)SMPs.,:SRT(ShinandKang,2003;Al-Halbounietal.,2008),F/M(Kimuraetal.,2005)HRT(Langenhoffetal.,2000),SVI(Garnieretal.,2005),SMPs.,,(Garnieretal.,2005;Barkeretal.,1999;Imaietal.,2002)EfOM,(Esparza-Sotoetal.,2006;WangandWu,2009),.SBR,,.2　(Materialsandmethods)2.1　水源水质情况,,1.1　Table1　PropertiesofthepracticalwastewatersourceCOD/(mg·L-1)DOC/(mg·L-1)BOD5/(mg·L-1)NH+4-N/(mg·L-1)TN/(mg·L-1)TP/(mg·L-1)pHAlkalinity/(mg·L-1,asCaCO3)240～42041.5～60.5200～28042.5～6250～703.7～8.57.21±0.40360±202.2　实验装置与运行条件SBR,-.14L,(28.0±1.0)℃,10d15d,MLSS3000mg·L-13500mg·L-1,2.,8h,5h、2h,45min15min.,1min,10L.2　SBRTable2　ParametersandoperationalconditionsoftheSBRF/MMLSS/(mg·L-1)SRT/dHRT/hSVI/(mL·g-1)SBR1#0.01230001010100SBR2#0.010350015101002.3　有机物分析方法4000r·min-110min,,0.45μm(MilliporeSwinnex-25),4℃,1.,2,2,,,,.2.3.1　DOC和SUVA　DOCTOC(MultiN/C3000,analytik-jena,),NPOC,,4℃,1.UV-7504PC,1cm254nm.SUVAUV254/DOC×100.2.3.2　碳水化合物　UV-7504PC,490nm.-,,3±10%.,(C6H10O5),21　　　　　　　　　　310.44.2.3.3　蛋白质　UV-7504PC,750nm.Lowry(Bio-RadDCProteinAssay),BSA(BovineSerumAlbumin,Bio-RadProteinAssayStandardII),3±4%.,(C16H24O5N4),0.55.2.3.4　单宁酸　UV-7504PC,700nm.5550-B(StandardMethodsfortheExaminationofWaterandWastewater),-,,.3±1%.2.3.5　分子量分布　(HPSEC),:(Waters1525),(Water2487),(Waters2475),,(WatersProtein-pak125column).(PSS,Fluka),210Da、1400Da、3400Da、13000Da、32000Da.,pH6.8,Na2SO40.1mol·L-1.20μL,0.7mL·min-1.254nm,278nm353nm,(Shonetal.,2006b;Parketal.,2006).3　(Results)3.1　SBR反应各阶段有机物分子量分布变化SBR,.1SBR.1a1b,SBR.-(MWD-UV),SBR,(MW500Da),1000Da,650Da、1700Da2000Da,(MW10000Da),,.-(MWD-FL),SBR,(MW500Da),(MW10000Da).,SBR,.1　SBRFig.1　MolecularweightdistributiontransformationofDOMduringSBRtreatment,1cSBR,EfOM-(MWD-UV)500～3000Da,221:SBR650Da、1000Da、1700Da2000Da,;500Da10000Da,.Esparza-Soto(2006),EfOM,500～3000Da.EfOM-(MWD-FL),100～2000Da10000Da,500～3000Da.EfOM(JarusutthirakandAmy,2006;Shonetal.,2006b),.SBR,,SBR2#650Da20000Da.3.2　SBR反应器出水溶解态有机物性质比较SBR,DOC、SUVA、、、.2　SBRSUVADOCFig.2　SUVAandDOCcomparisonbetweentheeffluentofSBR1#andSBR2#3.2.1　DOC和SUVA　2,SBRDOC11mg·L-19.5mg·L-1.,SBR2#(SRT=15d)SBR1#(SRT=10d),DOC,.,SUVA,,SBR2#SUVASBR1#,.,SBR2#SBR1#,(MLSS=3500mg·L-1),,.,.3.2.2　出水溶解性有机碳构成　SBR3,、、.,SBR,、SMPs70%～80%,.SMPs,,,.,SBR2#(SRT=15d)SBR1#(SRT=10d),,SMPs,.,SBR2#,,.3　SBRFig.3　DissolvedorganiccarbonconstitutentsofsewageeffluentfromSBR4　(Discussion)4.1　生物降解过程中有机物的转化3:MW10000Da(10000～30000Da),、;500DaMW3000Da,;MW200Da,23　　　　　　　　　　31(Shonetal.,2005).650Da、1000Da、1700Da2000Da,;10000Da,SMPs.650Da、1700Da2000Da(1),.,1MW1000