SBR中异养硝化菌的分离与硝化特征姚秀清

SBR姚秀清,　韩　明(辽宁石油化工大学环境与生物工程学院,辽宁抚顺113001)　　　:　在SBR反应器中,采用模拟废水和好氧活性污泥富集培养异养硝化菌,并对分离、筛选出的菌株进行脱氮性能的检测。在进水氨氮由100mg/L逐渐增至1396mg/L的过程中,对NH3-N的去除率始终能达到100%,而对TN的去除率由初期的零逐渐增至后期的45.7%,说明在后期发生了同步硝化反硝化作用(SND),系统中可能存在异养硝化菌。在筛选出的15株硝化菌中,菌株A1和T6经过1周的好氧培养后,对COD的去除率分别为56.0%和72.3%,对氨氮的去除率分别为71.2%和85.8%,且仅检测到痕量的亚硝酸盐氮和硝酸盐氮,由此证明菌株A1和T6为异养硝化菌。　　:　SBR;　异养硝化菌;　氨氮:X703　　:C　　:1000-4602(2010)23-0083-03CharacteristicsofHeterotrophicNitrifyingBacteriaIsolatedfromSBRYAOXiu-qing,　HANMing(SchoolofEnvironmentalandBiologicalEngineering,LiaoningUniversityofPetroleumandChemicalTechnology,Fushun113001,China)　　Abstract:　Simulatedwastewaterandaerobicactivatedsludgewereusedtocultivateheterotrophicnitrifyingbacteria,andthenitrogenremovalperformanceofstrainsseparatedandscreenedwasdetected.Duringtheincreaseoftheinfluentammonianitrogenfrom100mg/Lto1396mg/L,theremovalrateofammonianitrogenisalways100%,whiletheremovalrateoftotalnitrogenisincreasedfrom0to45.7%,whichindicatesthatthereareheterotrophicnitrifyingbacteriaandsimultaneousnitrificationanddenitrifi-cation(SND)intheSBR.Among15strainsscreened,after7dayculture,theremovalratesofCODbythestrainsA1andT6are56.0%and72.3%respectively,andtheremovalratesofammonianitrogenare71.2%and85.8%respectively.TracenitrateandnitritedetectedprovethatthestrainsA1andT6areheterotrophicnitrifyingbacteria.　　Keywords:　SBR;　heterotrophicnitrifyingbacteria;　ammonianitrogen　　,,。,1894StutzerHartleb。,[1、2]。,2～3,、,,[3、4]。,,,,,·83·第26卷　第23期2010年12月　　　　　　　　　　　　中国给水排水CHINAWATERWASTEWATER　　　　　　　　　　　　　Vol.26No.23Dec.2010。SBR,。1　试验部分1.1　SBR6L,5L,1。,DO2mg/L,NaHCO3(90g/L)pH7.2～8.0。,COD1～10kg/(m3·d),0.1～1.4kg/(m3·d)。1　Fig.1　Schematicdiagramofexperimentalset-up1.2　,pH7～7.5,:Na2HPO4·7H2O,7.9g/L;KH2PO4,1.5g/L;MgSO4·7H2O,0.1g/L;,2mL;(NH4)2SO4,COD。,pH=7.0,:EDTA,50.0g/L;ZnSO4,2.2g/L;CaCl2,5.5g/L;MnCl2·4H2O,5.06g/L;FeSO4·7H2O,5.0g/L;(NH4)6Mo7O24·4H2O,1.1g/L;CuSO4·5H2O,1.57g/L;CoCl2·6H2O,1.61g/L。SBR(),SV30=30%,,24h,,DO≥2.0mg/L,1396mg/L,5。1.3　,:50mL,2.4g/L(NH4)2SO416g/L,0.5mg/L。,:5g/LK2HPO4、2.5g/LMgSO4·7H2O、2.5g/LNaCl、0.05g/LFeSO4·7H2O、0.05g/LMnSO4·4H2O,pH7.8;pH。,pH,。20g/L。SBR1mL、4mL10,30min,。100μL,(30±2)℃3d。,5dGriess-losvay。,。1.4　COD:;:;:Griess;:NEDD。Giltay。GiltayA、B。A:KNO3,1.0g;,1.0g;1%(BTB),5mL;,500mL。B:,8.5g;MgSO4·7H2O,1.0g;FeCl3·6H2O,0.05g;KH2PO4,1.0g;CaCl2·2H2O,0.2g;,500mL。A、BpH7.0～7.2,。2　结果与讨论2.1　SBRSBRNH3-N100mg/L200、300、400、532、691、833、930、11751396mg/L,NH3-N,100%,。,NH3-N≤532mg/L,NH3-N100%,TN,NH3-N;NH3-N532mg/L,,45.7%,(SND)。,,。2.2　15,NH3-N·84·第26卷　第23期　　　　　　　　　　　　　　中国给水排水　　　　　　　　　　　　。A1T6,2d,;3d,;3～4d,,,。1　Tab.1　NitrificationanddenitrificationactivityofstrainsNO-2-NNO-3-NA1++++A2+-A3+++A4--A5+-A6++-A7--A8--T1+-T2+++T3++-T4--T5+-T6++++T7+-　:　“-”0.1mg/L;“+”0.1～0.5mg/L;“++”0.5～1mg/L;“+++”1～2mg/L。2.3　,1,T6A1,COD,72.3%56.0%;NH3-N,185.8%71.2%(2)。,,,。,()。,3d,,。Giltay,T6A1,。2　Fig.2　VariationofNH3-Nconcentrationduringcultureperiod3　结论①　SBR100mg/L1396mg/L,NH3-N≤532mg/L,100%,TN;NH3-N532mg/L,NH3-N,TN,45.7%,(SND),。②　15,A1T61,COD56.0%72.3%,71.2%85.8%,,A1T6。:[1]　,.[J].,2003,25(5):283-285.[2]　,,,.[J].,2006,22(21):67-70.[3]　,,,.[J].:,2003,25(5):107-110.[4]　BrierleyEDR,WoodM.Heterotrophicnitrificationinanacidforestsoil:isolationandcharacterisationofanitrif-yingbacterium[J].SoilBiolBiochem,2001,33(10):1403-1409.E-mail:[e-mail]:2010-06-03·85·姚秀清,等:SBR中异养硝化菌的分离与硝化特征第26卷　第23期