SCAR处理城市生活污水的效能及其微生物群落动态分析杨波

38520175ENVIＲONMENTALSCIENCEVol．38No．5May2017SCAＲ1212121212121．2016202．201620SCAＲ、．HＲT6hCOD75%．F420SMAEPSEPSTB-EPS．HＲT．SCAＲX172X703.1A0250-3301201705-2021-09DOI10．13227/j．hjkx．2016090242016-09-042016-10-2413ZＲ14010001973～[e-mail]ndMicrobialCommunityDynamicsofaStrengthenCirculationAnaerobicＲeactorTreatingMunicipalWastewaterYANGBo12XUHui12FENGXiu-ping12LIFang12TIANQing12MAChun-yan121．StateEnvironmentalProtectionEngineeringCenterforPollutionTreatmentandControlinTextileIndustryShanghai201620China2．CollegeofEnvironmentalScienceandEngineeringDonghuaUniversityShanghai201620ChinaAbstractAstrengthencirculationanaerobicreactorSCAＲtreatingartificialmunicipalwastewaterwasinvestigatedunderdifferentvolumetricloadingrateVLＲandthereactorperformancecharacteristicsofgranularsludgeandmicrobialcommunitystructurewerealsotestedinthisexperiment．TheresultsoftheexperimentdemonstratedthatthehydraulicretentiontimeHＲTof6hcouldberegardedasthekeyparameterdominatingtheefficientoperationofSCAＲreactorinwhichconditiontheCODremovalefficiencywasabove75%．ThecoenzymeF420andthemaximumspecificmethanogenicactivitySMAofgranularsludgeincreasedwithincreasingVLＲandtheEPScontentsespeciallyTB-EPSinthegranulesludgealsoincreasedobviously．ConsistentlythecharacteristicsofanaerobicgranularsludgeandtheremovalefficiencyofthereactorwereinfluencedbybothsludgeloadingandHＲT．Themicrobialcommunitystructureanditsspatialdistributioninthereactorwerealsoaffectedbysludgeloadingwhiletherelativeabundanceofthemicrobialcommunitywithdifferentmetaboliccharacteristicsindifferentspatialpositionschangedwiththeadjustmentofthesludgeloading．KeywordsstrengthencirculationanaerobicreactorSCAＲmunicipalwastewaterparticlesizedistributionactivityofsludgeEPSmicrobialcommunity1～3．、、4．5．6～8．910．SCAＲ38Vup．SCAＲ．HＲT、SMAF420EPS．、、．11.1COD450mg·L－128mg·L－1、57mg·L－15mg·L－1mg·L－1111122001003010010305NH4Cl100K2HPO420CaCl25FeSO4·7H2O5.8MgSO4·7H2O6．pHNaHCO3．1.2IC．2.19mmMLSS48.45g·L－1MLVSS/MLSS0.79．28L2/5．1.31．1SCAＲFig．1SchematicdiagramoftheSCAＲsystemtreatingmunicipalwastewaterSCAＲ0.2m2.0m70L．525、60、100、140170cmA、B、C、D、E．30℃±1℃．120d100d20d．Vup4m·h－1HＲT451213．22025SCAＲ1.4COD、MLSS、MLVSS14F42015SMA1216VFASgc789015m×0.53mmFFAPpH＜2EPS17-18BCA19．．E．Z．N．ATMMag-BindSoilDNAKitOMEGADNA．Qubit2.0DNALifeDNAPCＲDNA．16SrDNAV3-V4．PCＲ341F-805ＲPCＲM-349FGU1ST-1000ＲPCＲ349F-806Ｒ．PCＲ．Qubit2.0DNA1∶1．Barcode．97%OTUOTUＲDP．．22.1SCAＲSCAＲHＲT=15h、0.7kg·m3·d－1．20d20dCOD75%VFAsSCAＲ．HＲTSCAＲ40.7kg·m3·d－10.89、1.18、1.772.7kg·m3·d－1HＲT12、9、64h．2aHＲT12、96h1～3dCODCOD．HＲTCODCOD75%．HＲT4h20dCOD107mg·L－1209mg·L－1COD75.3%50.8%VFAsHＲT．HＲT12hCOD75%．SCAＲHＲT=6hSCAＲ．VFAs、HＲT20．2bHＲTVFAsHＲT15、12、96hHＲT4hVFAs．HＲT21．2bHＲTHＲT6h．CO2、22．2.2SCAＲ2.2.1320238aCOD、COD、b2SCAＲHＲTFig．2PerformanceofSCAＲunderdifferentHＲTconditions3SCAＲHＲT3．＞1mm90%＞2mm60%HＲT=12h＞1mm80%＞2mm47%HＲT=6h＞1mm81%1～2mm30%44%HＲT=4h＞1mm86%1～2mm52%＜1mm5%．＜1mm＞2mm1～2mm．Vup．＞1mm．42025SCAＲ3SCAＲFig．3ParticlessizedistributionandsludgeconcentrationoftheSCAＲsystemduringtheoperationprocess52.19g·L－110d45.34g·L－1HＲT=9h39.29g·L－1．HＲT=6h46.15g·L－1．HＲT=6hSCAＲ．2.2.2SMAF420F420SMA1523HＲTADF420SMA4．4ADF420SMA．HＲTF420SMAHＲT6h4hF420F420．4F420Fig．4VariationofspecificmethanogenicactivityandcoenzymeF420ofgranularsludgewithHＲT．4DSMAA．2.2.3EPSEPSPS、PN、．EPS24．520238HＲTEPSEPSS-EPS、EPSLB-EPS、EPSTB-EPS5．5EPSEPSTB-EPS．HＲT=15h3EPSEPSTB-EPS4.48mg·g－1EPS．S-EPS、LB-EPS、TB-EPSEPS．5HＲTEPSFig．5EPScontentintheinoculumandgranulesofSCAＲatdifferentHＲTTB-EPS25．TB-EPSHＲT=15h4.48mg·g－1HＲT=4h10.19mg·g－1．EPSPNPS24～264HＲT15、12、96hEPSPN/PS4HＲT=4hEPSPN/PSTB-EPSPN/PSHＲT=6h4.361.27．HＲT=4hLB-EPSHＲT=6h2.07mg·g－15.38mg·g－1EPSLB-EPS2526．2.3HＲT=12hHＲT=6h6、78HＲT=12hHＲT=6h．6Chloroflexi、Bacteroidetes、Firmicutes、Proteobacteria．Ambuchi27EGSBexpandedgranularsludgebedChloroflexiFirmicutesLiao28EGSBProteobacteria．HＲT12h6h6Chloroflexi28.89%16.49%Proteobacteria17.96%26.43%．ChloroflexiChloroflexi29ProteobacteriaChloroflexi．Methanosaetasp．36.2%、Methanolineasp．29.1%、Methanosarcinasp．9.7%、Methanomassiliicoccus4.2%、Methanobacterium4.9%．Methanosaetasp．Methanolinea62025SCAＲA1HＲT=12hAD1HＲT=12hDA2HＲT=6hAD2HＲT=6hD6Fig．6ＲelativeabundanceofbacterialphylaA1HＲT=12hAD1HＲT=12hDA2HＲT=6hAD2HＲT=6hD7Fig．7Ｒelativeabundanceofarchaealgenerasp．50%80%．Chelliapan210.86～1.86kg·m3·d－1．Methanosaeta130～33．HＲT=12hMethanosaeta16.08%HＲT=6hMethanosaeta75.58%．Siggins34EGSBVFAMethanosaeta．8SCAＲ80mg·L－1Methanosaeta2m、HＲT=12h157202388SCAＲFig．8VariationsofaceticacidalongtheheightofSCAＲmg·L－1HＲT=6h40mg·L－1Methanosaeta15mg·L－1Methanosaeta．Wang35EGSB10kg·m3·d－1．Methanolinea30Methanosaeta．、364SMAF420．31SCAＲ4m·h－130℃．SMAF420．HＲT=6h、1.77kg·m3·d－1COD75%HＲT4hVFAs．21～2mm．EPSTB-EPSEPSPN．3．ChloroflexiProteobacteriaMethanolineasp．Methanosaetasp．．1OzgunHGimenezJBErsahinMEetal．ImpactofmembraneadditionforeffluentextractionontheperformanceandsludgecharacteristicsofupflowanaerobicsludgeblanketreactorstreatingmunicipalwastewaterJ．JournalofMembraneScience201547995-104．2HahnMJFigueroaLA．Pilotscaleapplicationofanaerobicbaffledreactorforbiologicallyenhancedprimarytreat