UASB反应器厌氧氨氧化菌的脱氮特性研究朱明石

2　1环境工程学报Vol.2,No.120081ChineseJournalofEnvironmentalEngineeringJan.2008UASB朱明石　周少奇　曾　武(,510006)　　UASB(ANAMMOX),,,pH7.4～7.8,(32±1)℃。270～450d180d,NH+4-NNO-2-N99.9%,90%,NO-3-N20～30mg/L。,UASB,NH+4-N、NO-2-NTN,NO-2-NNH+4-N。UASB,ANAMMOX,,。pH8.5,pH。　　　　　X703.3　　　A　　　1673-9108(2008)01-0011-05CharacteristicsofanaerobicammoniumoxidationbacteriafromUASBreactorZhuMingshi　ZhouShaoqi　ZengWu(CollegeofEnvironmentalScienceandEngineering,SouthChinaUniversityofTechnology,Guangzhou510006)Abstract　Theanaerobicammoniumoxidation(ANAMMOX)processandoperationalcharacteristicsinaupflowanaerobicsludgeblanket(UASB)reactorwerestudied.Thenitrificationbio-filmwascultivatedwithacti-vatedsludgefromamunicipalsewagetreatmentplant.TheUASBreactorwasfedwiththesyntheticwastewaterscontainedhighconcentrationsofammoniumnitrogen(NH+4-N)andnitritenitrogen(NO-2-N),thepHvaluewasfixedat7.4to7.8andtemperatureat(32±1)℃.TheUASBreactorwasoperatedduringaround180daysfromdays270to450ofoperationinthisstudy.TheremovalratesofNH+4-NandNO-2-Nwereboth99.9%;o-ver90%ofthetotalnitrogen(TN)wereremoved,andthenitratenitrogen(NO-3-N)productionratewasbe-tween20to30mg/L.ThestudyindicatedthattheefficiencyofANAMMOXreactioninUASBreactorwassignifi-cant,ithadhighremovalratesofNH+4-N,NO-2-NandTN.TheratioofNO-2-NtoNH+4-NcouldindicatetheoperationoftheANAMMOXreactors.Atthestablestageofthereaction,theeffectoftheinflowratewasnotsig-nificant,ANAMMOXbacteriaperformedwell.TheeffluentpHwaskeptaround8.5andastheindicationsignoftheoperation.Keywords　nitrification;ANAMMOX;UASB;biologicalnitrogenremoval:(20377013);(020959);:2007-07-21;:2007-10-09:(1982～),,,。E-mail:[e-mail]　　,,。,,。、、、。,、。,、、———(anaerobicammoniaoxidation,ANAMMOX)。Broda,1977[1]。1995,Mulder[2]。2NO-2,(ANAMMOX)(N2)[3]。[4～8],[9～13]。(upflowanaerobicsludgeblanket,UASB),,。1　1.1　,3.2L,2.28L,0.92L。1[14]。1　Fig.1　Experimentalapparatusandprocess1.2　,1。1　Table1　Compositionofthefeedingmineralmediamg/LNH4ClNaNO2MgSO4300KH2PO430CaCl2136NaHCO3500Ⅰ(ml/L)1.0Ⅱ(ml/L)1.02。2　Table2　Compositionoftraceelementsinsolutionmg/LⅠFeSO45000ⅡZnSO4·7H2O430CoCl2·6H2O240MnCl2·4H2O990CuSO4·5H2O250NiCl2·6H2O190H3BO3141.3　3。3　Table3　AnalyticalmethodsNH+4-NNO-2-NN-(1-)-NO-3-NpH2　2.1　、,。270d。(32±1)℃,NaHCO3pH7.4～7.8。,。10d(23),NH+4-N、NO-2-N,,NH+4-NNO-2-N,,,。30d,,,。121:UASB2　、Fig.2　VariationofNH+4-NandNO-2-Nduringtheoperation3　、NO-3-NFig.3　VariationofNO-3-Nduringtheoperation　　2,,NH+4-NNO-2-N99.9%,90%,NO-3-N20～30mg/L,NH+4-NNO-3-N0.26[15],,。130～180d,,,NO-3-N,,(SND)[16],NO-3-N,,。2.2　2.2.1　NH+4-N,NO+3-N的影响NO-2-NNH+4-N。[17],NO-2-NNH+4-Nσ,TN,[σ1,σ2],σ1σσ2。,ANAMMOX,TN,σ=σTNmax,。ANAMMOXNO-2-NNO-3-N,NO-2-N2,NO-3-N,NH+4-N[18]。,NO-2-NNH+4-N。4,NO-2-NNH+4-Nσ1.5,1.3～1.8,90%。50dNO-2-NNH+4-Nσ,3.0,,,,,,85%。,NO-2-NNH+4-Nσ,1.5,,,。,。,NO-2-NNH+4-Nσ=1.5,[1.3,1.8],。4　NO-2-NNH+4-NFig.4　RatioofNO-2-NtoNH+4-NandTNremovalrate2.2.2　容积负荷的影响。,。(DO),DOAN-132AMMOX[19],。5,,NH+4-N、NO-2-NTN,,TN0.3kg/(m3·d)。UASB,,。5　Fig.5　Variationofnitrogenremovalrateandloads2.2.3　pH值的影响pH。pH,;,pH,。,UASB,pH,pH。6,pH8.5,,pH8.0[20]。,pHNH+4-N、NO-2-NTN。,TNpH,pHTN。pH[6]。3　(1)UASB,270～450d180d,NH+4-N、NO-2-N99.9%,90%6　TNpHFig.6　VariationofpHvalueandTNremovalrate,NO-3-N20～30mg/L。(2)NO-2-NNH+4-Nσ=1.5,[1.3,1.8],。(3)UASB,,,。pH8.5,pH。UASB,,。、、DO、。[1]BrodaE.Twokindsoflithotrophsmissinginnature.Z.AllgMicrobiol.,1977,17:491～493[2]MulderA.,vandeGraafA.A.,RobertsonL.A.,etal.Anaerobicammoniumoxidationdiscoveredinadenitrifyingfluidizedbedreactor.FEMSMicrobiol.Ecol.,1995,16(3):177～183[3]VandeGraafA.A.,deBruijnP.,RobertsonL.A.,etal.Metabolicpathwayofanaerobicammoniumoxidationonthebasisof15Nstudiesinafluidizedbedreactor.Microbi-ol.,1997,143(7):2415～2421[4]MikeS.M.Jetten.Newpathwaysforammoniaconversioninsoilandaquaticsystems.PlantandSoil,2001,230:9～19[5]MikeS.M.Jetten,MichaelWagner,JohnFuerst,etal.Microbiologyandapplicationoftheanaerobicammonium141:UASBoxidation(anammox)process.CurrentOpinioninBiotech-nology,2001,12:283～288[6]TohS.K.,WebbR.I.,AshboltN.J.Enrichmentofauto-trophicanaerobicammonium-oxidizingconsortiafromvari-ouswastewaters.Microb.Ecol.,2002,43:154～167[7]ThirdK.A.,PaxmanJ.,StrousM.,etal.EnrichmentofanammoxfromactivatedsludgeanditsapplicationintheCANONprocess.Microb.Ecol.,2005,49:236～244[8]KimWindey,IngeDeBo,WillyVerstraete.Oxygen-limit-edautotrophicnitrification-denitrification(OLAND)inarotatingbiologicalwastewater.WaterResearch,2005,39:4512～4520[9]..(),2000,28(11):16～19[10],,.UASB.,2003,22(10):665～669[11],,,.Anammox.,2005,25(4):545～551[12],,,..,2005,24(6):629～631[13],..,2005,56(10):1826～1831[14],.UASB.,2005,24(6):1～5[15]StrousM.,HeijnenJ.J.,KuenenJ.G.,etal.These-quencingbatchreactorasapowerfultoolforthestudyofslowlygrowinganaerobicammonium-oxidizingmicroorgan-isms.Appl.Microbiol.Biotechnol.,1998,50(5):589～596[16]..(),2006,34(5):1～4[17],,.ANAM-MOX.,2006,32(5):27～33[18],,..,2005,26(2):137～141[19]XiaodiHao,JosephJ.Heijnen,MarkC.M.VanLoos-drecht.Model-basedevaluationoftemperatureandinflowvariationsonapartialnitrification-ANAMMOXbiofilmprocess.WaterResearch,2002,36:4839～4849[20]EgliK.,FangerU.,AlvarezP.J.J.,etal.Enrichmentandcharact