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2000,6(5):473～476 ChinJApplEnvironBiol=ISSN1006-687X 2000-10-25 :2000-05-15 :2000-07-27＊2000.＊＊(Correspondingauthor)UASBEGSB＊周洪波 陈 坚＊＊ 任洪强 丁丽丽 华兆哲( 214036) 2LEGSBUASB、F420.,EGSB、UASB.UASBF420EGSB.F420.,,.2213 UASB;EGSB;;CLC TQ921.7:X703COMPARATIVESTUDYONTHEBIOLOGICALCHARACTERISTICSOFANAEROBICGRANULARSLUDGESFROMUASBANDEGSBREACTORSZHOUHongbo,CHENJian＊＊,RENHongqiang,DINGLili&HUAZhaozhe(LabofEnvironmentalBiotechnology,SchoolofBiotechnology,WuxiUniversityofLightIndustry,Wuxi,Jiangsu,214036,China)Abstract Thespecificmethaneproductionactivities,contentsofcoenzymeF420andextracelluarpolymersweredeterminedinanaerobicgranularsludgesculturedin2Lbench-scaleEGSBandUASBreactors.ResultsshowedthatinsludgefromEGSBreactor,thespecificmethaneproductionactivityinglucose,theactivitiesofacetate-utilizingmethanogensandhydrogen-producingacetogensandcontentofextracellularpolymerswereap-parentlyhigherthanthoseinsludgefromUASBreactor.However,theactivitiesofformate-utilizingandhydro-gen-utilizingmethanogens,andcontentofcoenzymeF420insludgefromUASBreactorwerehigherthanthoseinsludgefromEGSBreactor.ThecontentofcoenzymeF420couldindicatetheactivityofmethanogensinthesamekindofsludge.Insludgesdepositedfor3months,littleactivityofhydrogen-producingacetogenswasdeter-mined,whereastheactivitiesofformate-utilizingandhydrogen-utilizingmethanogenswerelessaffectedbystor-age.Fig2,Tab2,Ref13Keywords UASB;EGSB;anaerobicgranularsludge;biologicalcharacteristicsCLC TQ921.7:X703(UpflowAnaerobicSludgesBed,UASB)2070.UASB、,UASB(ρt(COD)=15kgm3d-1),10,67%[1].(ExpandedGranularSludgeBed,EGSB)UASB,:(1),;(2),,;(3)COD,,;(4)(h/d),,[2,3].,EGSB,EGSB,EGSB.UASBEGSB、,、,.、,EGSB.1 1.1 1.1.1 UASB,2LUASB2LEGSB(1).35℃,.(tHR)UASB24h,EGSB,24h..[4].,3.1.1.2 (ρ/gL-1) 2,(NH4)2CO30.04,CaCl20.004,KH2PO40.04,0.08,NH4Cl0.04,MgSO40.008,1L.[5]1mLL-1.pHNaHCO36.8～7.0.1.2 1.2.1 [6].1 Fig.1 Schmaticofexperimentalapparatusforanaerobicsludgeculture1-EGSB;2-UASB;3-;4-;5-;6-;7-;8-;9-1-EGSBreactor;2-UASBreactor;3-Feedreservior;4-Pump;5-Heater;6-Inlet;7-Outlet;8-Three-phaseseparator;9-Samplingpoint1.2.2 F420 [6].1.2.3 [7].1.2.4 VSS [8].2 2.1 (UASBEGSB)(),、、、、、.1.,,1.1,EGSBUASB,1.5.,EGSB.,UASB,EGSB474 6,COD.1UASBEGSB,UASBEGSB,50%[9].EGSB,EGSB.EGSB、UASB,EGSB.EGSB,,,,,,、[10],、.1 [Rmax(CH4)/mLg-1d-1]Table1 MaximumspecificmethaneproductionratesofgranularsludgesindifferentsubstratesSludgeGlucoseFormateAcetatePropionateButyrateValerateLactateUASB(N)200.3344.459.066.635.822.679.1EGSB(N)315.6191.165.5106.755.523.5168.1UASB(O)135.3280.644.6LLLLEGSB(O)210.4145.650.1LLLL N:;O:3;L:. N:freshsludge;O:sludgedepositedfor3months;L:Littleactivity.,、、,.,,,[11].2.2 F420 F420[12].UASBEGSBF420(2),UASBF420EGSBF420,2.,F420[12],F420,,UASB,EGSB.,UASBEGSB,F420.F420,.,F420,.Dolfing[12].2.3 ,,,,,,,[13].UASBEGSB2.2,EGSBUASB,1.5.,UASB,4755:UASBEGSB EGSB.UASBEGSB0.85mm1.78mm[4],. UASBEGSB,,,、,..2 (w/mgg-1)Table2 Contentsofextracellularpolymersindifferentgranularsludges(w/mgg-1)SludgeTotalextracellularpolymerPolysaccharideProteinNucleicacidUASB(N)67.15.935.226.0EGSB(N)94.13.451.339.4UASB(O)112.58.064.240.3EGSB(O)120.18.459.552.23 (1)EGSBUASB,315.6200.3mLg-1d-1.UASB,EGSB.,.(2)UASBF420EGSB,0.1660.1μmolg-1.UASB,F420.,F420.(3)EGSB(w)UASB,94.167.1mgg-1.,UASB,EGSB.1 RenHQ(),DingLL(),ChenJ(),LunSY().Areviewofthedevelopmentofanaerobicbioreactorforwastewatertreatment.JWuxiUniLightIndustry().1999,18(suppl):169～1722 LettingaG.Sustainableintegratedbiologicalwastewatertreatment.WatSciTech.1996,33(3):85～983 LettingaG,RebacS,ParshinaS.High-rateanaerobictreatmentofwastewateratlowtemperatures.AppEnvironMicrobiol.1999,65(4):1696～17024 RenHQ(),DingLL(),ChenJ(),LunSY().StudyonthephysicalcharacteristicsofgranularsludgefromExpandedgranularsludgebed(EGSB)(accepted).ProceedingsofChina-JapanJointSymposium(),22～24Sep,2000,Beijing,China.5 ZehnderAJB,HuserBA,WuhrmannK.Characterizationofanacetate-decarboxylating,non-oxidizingmethanebacteria.ArchMicrobi-ol.1980,124(1):1～116 ,,..:,19907 LiuZJ(),XieH(),YuYX().Selectionofmethodsforextractionanddeterminationofextracellularpolymersfromanaerobicsludge.EnvironSci().1994,15(4):23～268 .(3).:,19899 DolfingJ,BloemenWGBM.Activitymeasurementsasatooltocharacterizethemicrobialcompositionofmethanogenicenvironments.JMicrobiolMethods.1985,4:1～1210 StrongGE,Cord-RuwischR.Aninsitudissolved-hydrogenprobeformonitoringanaerobicdigestersunderoverloadingconditions.Biotechnol.Bioeng.1995,45:63～6811 ZhaoYH(),QianZS().Studyonmicrobialecologyofanaerobicfilterusedfortreatmentofslaughterwastewater.Chi-naBiogas().1987,5(2):6～1412 DolfingJan,MulderJan-Willem.ComparisonofmethaneproductionrateandcoenzymeF420contentofmethanogenicconsortiainanaero-bicgranularsludge.AppEnvironMicrobiol.1985,49:1142～114513 SchmidtJensE,AhringBrigitteK.Granularsludgeformationinupflowanaerobicsludgeblanket(UASB)reactors.BiotechnolBioeng.1996,49:229～246476 6