UASB生物制氢反应系统生物强化作用研究龙敏南

47　5厦门大学学报(自然科学版)Vol.47　No.5　20089JournalofXiamenUniversity(NaturalScience)Sep.2008　·快　讯·UASB龙敏南,史继祥,徐惠娟,邬小兵,胡　忠,徐方成(,,361005)　　:2008-03-18:(C0410002),(2005I016,2006H0091),Email:[e-mail]:(UASB),,.HP1,.:30.0gVSS/L、6.0kgCOD/(m3·d)、(HRT)9h、3%,25%,,63%,40%～52%,4.52L/d.:UASB;;;:Q935;Q936　　:A　　　　　:0438-0479(2008)05-0620-04　　(Bioaugmentation),.,,,,,,.Saravance[1],COD,COD88.5%.Guiot[2],36d,100%,171d.[3](CSTR),,,.,,(UpflowAnaerobicSludgeBed,UASB),,.(Klebsiellaoxytoca)HP1UASB,.1　1.1　(KlebsiellaoxytocaHP1),[4].1.2　　1　UASBFig.1　SchematicdiagramoftheUASBbiohydrogenpro-ductionreactor,、、,COD2000mg/L,3,,.1.3　2,6.7L,1.,、、,...1.4　,,V(CO):V(N):V(P)1000:5:1,N、P.1.5　COD:[5].(VSS):[5],.:102G,,;99.999%.:VARIANCP-3800.30m×0.32mm,DF0.25μmcp8843;()2mL/min;()30mL/min;()300mL/min.300℃,300℃,1μL.1.6　III,IHP1,;IIHP1,3%,10d.,30.0gVSS/L,6.0kgCOD/(m3·d),6.0kgCOD/(m3·d),30.0kgCOD/(m3·d),HRT9h,5d.2　2.1　,,.UASB,2.,,10d,21～30d,9.22L/d.　2　◇　I;—◆—IIFig.2　Changesofbiohydrogen,biogasandhydrogencontentduringreactorstart-up,,,.15d,,,,4.52L/d,4.14L/d,25%.,,40%～52%,44%,8%.,10d,,.,.3.·621·5　　　　　　　　　　　　　:UASB　3　Fig.3　Effectofbioaugmentationonbiohydrogenpro-ductionability,.,18.38mL/(gVSS·d),12.77mL/(gVSS·d).,,44%.2.2　pH12.、、、,.,.,,20d,.,.,63.40mmol/L,40.34mmol/L,63.6%.,53.47mmol/L,33.51mmol/L,62.7%.,,1.2.,　4　pHFig.4　EffectofbioaugmentationonpHvalueduringre-actorstart-up.pH,pH4.,pHpH.pH4.0～4.3,pH4.4～4.61　　Tab.1　Compositionoffermentationproductsduringstart-upwithoutaugmentation/d/(mmol·L-1)04.395.444.394.342.3556.458.195.795.603.48109.2610.256.618.344.11159.3813.717.329.935.14209.2816.455.9013.944.56259.2718.895.8713.364.75309.6719.795.6213.724.672　　Tab.2　Compositionoffermentationproductsduringstart-upwithaugmentation/d/(mmol·L-1)04.616.402.794.571.3656.188.864.756.402.51108.4110.756.098.823.35159.0515.037.1811.324.77209.8515.886.3813.654.54259.7419.266.2115.694.953011.0323.076.9117.275.12·622·()　　　　　　　　　　　　　　　　　　2008,,,,,pH.3　　(1)UASB,25%,8%.44%.(2)UASB,,.,,1.2.(3)HP1UASB,,,HP1UASB.:[1]　SaravananeR,MurthyDVS,KrishnaiahK.Bioaugment-ationandtreatmentofcephalexindrug-basedpharmaceu-ticaleffluentinanupflowanaerobicfluidizedbedsystem[J].BioresourceTechnology,2001,76:279-281.[2]　GuiotSR,Tawfiki-HajjiK,LepineF.Immobilizationstrategiesforbioaugmentationofanaerobicreactorstrea-tingphenoliccompounds[J].WaterScienceandTechnolo-gy,2000,42:245-250.[3]　,,.[J].,2004,25(6):113-116.[4]　MinnanL,JinliH,XiaobinW,etal.Isolationandcharac-terizationofahighH2-producingstrainKlebsiellaoxyto-caHP1fromahotspring[J].ResearchinMicrobiology,2005,156:76-81.[5]　.[M].3.:,1989.BioaugmentationStudyonUASBBiohydrogenProductionSystemLONGMin-nan,SHIJi-xiang,XUHui-juan,WUXiao-bing,HUZhong,XUFang-cheng(SchoolofLifeSciences,ResearchInstituteofEnergy,XiamenUniversity,Xiamen361005,China)Abstract:BioaugmentationwithhydrogenproducingstrainKlebsiellaoxytocaHP1inup-flowanaerobicsludgebed(UASB)re-actorwasstudied.Theoptimumstart-upconditionforreactorwasobtainedas:sludgeinoculantsof30.0gVSS/L,initialorganicloadingrateof6.0kgCOD/(m3·d),hydraulicretentiontimeof9h,andbacteriadosinginoculantsof3%.Undertheoptimalcondi-tions,thehydrogenproductionincreasedby25%,thehydrogencontentinthebiogaswasabout40%～52%,themaximalhydrogenratewas4.52L/d.Keywords:UASB;biohydrogenproduction;hydrogenproducingstrain;bioaugmentation·623·5　　　　　　　　　　　　　:UASB