阿维菌素废水工业化UASB颗粒污泥产甲烷菌群分析1

31320103ENVIRONMENTALSCIENCEVo.l31,No.3Mar.,2010UASB1,1,2,1*,1,1(11,050018;21,100862):,(fluorescenceinsituhybridization,FISH)UASB.,(),(methanogens)(Methanobacteriales)(Methanosarcinales),.;;(25150?8163)%~(48167?8187)%;110~210mm,(47108?8126)%(48167?8187)%..11311~11562g/(g#d),COD,,.:;;;;:X172:A:0250-3301(2010)03-0725-06:2009-05-11;:2009-06-08:(50878072);(E2008000694,E2009000709):(1976~),,,,,E-mai:[e-mail]*,E-mai:[e-mail]CommunityofAnaerobicGranularSludgeinaFul-lscaleUASBTreatingAvermectinWastewaterLIUChun1,MAJun-ke1,WUGen2,YANGJing-liang1,GUOJian-bo1,LIZa-ixing1(11SchoolofEnvironmentalScienceandEngineering,HebeiUniversityofScienceandTechnology,Shijiazhuang050018,China;21BasicResearchServiceCenter,MinistryofScienceandTechnology,Beijing100862,China)Abstract:Methanogensisconsideredtobeimportantfunctionalmicrobialpopulationinanaerobicgranularsludge.Fluorescenceinsituhybridizationwasusedtoanalyzemethanogensofanaerobicgranularsludgeinaful-lscaleUASBtreatingavermectinwastewater.Theresultsindicatedthatthedistributionformsofmethanogens,MethanobacterialesandMethanosarcinaleswereidenticalonthesurfaceandinnerfaceofgranularsludgesamplesindifferentformationstages(withdifferentdiameters),althoughtherelativeabundancesofthesemethanogensweredifferent.Therelativeabundancesofmethanogensontheinnerfacewerelargerthantheseonthesurfaceofgranularsludgesamples.TherelativeabundancesofMethanobacterialeswerelargerthantheseofMethanosarcinales.Therelativeabundancesofmethanogensinallgranularsludgesamplesrangedfrom(25150?8163)%to(48167?8187)%.Themaximumrelativeabundancesofmethanogenswereobtainedinmaturegranularsludgewithdiameterof110-210mm,(47108?8126)%onthesurfaceand(48167?8187)%ontheinnerface,respectively.Theavermectinresidueinwastewatershowedpossibleinhibitioneffectonmethanogens.Themaximumspecificmethanogenicactivitiesofgranularsludgesamplesrangedfrom11311to11562g/(g#d),varyingassameasCODremovalratiosandrelativeabundancesofmethanogens,implyingthestrongcorrelationofmethanogenswithbioactivityofgranularsludge.Keywords:anaerobicgranularsludge;methanogens;fluorescenceinsituhybridization(FISH);relativeabundance;avermectinwastewater,,[1~4].,.,(FISH),[5,6].[7~9]FISH,,,.Montero[10]FISH..,31[11,12][13].,,,.Chen[14];Liu[15]PCPPCP.,.,.,,.UASB,FISH,.1111UASB(500m3),VSS/SS01941.UASB016mm016~110mm110~210mm210mm4,1418%513%6013%1916%,VSS/SS01905019400195701945.110~210mm,;016~110mm;210mm;016mm.112FISH112114:UNIV1392MPB1MEB859MSSH859,,1.(Takara).11212250LL115mL,34%.4e1h,10000r/min5min,,1@PBS3,,50%80%100%,3min.1LL011nmol/LL9LL(019mol/LNaC,l0101%SDS,0102mol/LTris-HC,l,pH712),,(UVP,HB-1000,),46e5h.1FISHTable1ProbesusedinFISH(5c-3c)UNIV1392ACGGGCGGTGTGTAC5cFITC[8]MPB1CATGCACCACCTCTCAGC5cFITC[12]MEB859GGACTTAACAGCTTCCCT5cFITC(5c)[16]MSSH859CTCACCCATACCTCACTCGGG5cFITC[16]46e(0101%SDS,0102mol/LTris-HC,lNaC,lpH712),46eddH2O3,.:,.11213(Motic,BA200,),30FISH(Moticam2206).MoticFluo110,,,(1)[17].(%)=@100%(1)113:(VSS)4,35e,.,7263:UASB:1%1%0125%0125%0105%NH4Cl0102%K2PO401005%MgCl2(),pH712,COD4000~5500mg/L[18],116~212kg/(m3#d).,K,(2)A[19].A=24@K@fV@S(2),A,g/(g#d);K,L/h;f,g/L,21574;V,L;S,g/L.COD:8hCOD,.2211211113(Methanogens)(Methanobacteriales)(Methanosarcinales),.,,.1110~210mm.;,[1(d)].24..,,.,,3.,,,110~210mm,210mm.,016mm016~110mm110~210mm210mm4(25150?8163)%(39148(a);(b);(c);(d)()()1110~210mmFig.1Distributionsofdifferentmethanogensonthesurfaceofsludgesamplewithdiameterof110-210mm(a)016mm;(b)016~110mm;(c)110~210mm;(d)210mm2Fig.2DistributionofMethanogensonthesurfaceofsludgesampleswithdifferentdiameters?9105)%(47108?8126)%(43169?9126)%.,,;110~210mm,;,.,,.110~210mm,(43168?7198)%(35131?6124)%,727313Fig.3Relativeabundancesofdifferentmethanogensonthesurfaceofgranularsludge(47108?8126)%..,110~210mm1123,,.21112110~210mm210mm,FISH,,.4110~210mm.,,5.,.110~210mm210mm,110~210mm210mm(48167?8187)%(45101?8197)%;,,110~210mm(44194?8178)%(37187?6158)%,1118,210mm.,,110~210mm(37187?6158)%(35131?6124)%.Jupraputtasri[12]FISH,(5017?619)%;Liu[8](Slo-tblothybridization)rRNA,rRNA6214%..,40mg/L[20],..(a);(b)4110~210mmFig.4DistributionofMethanosarcinalesontheinnerfaceandsurfaceofsludgesamplewithdiameterof110-210mm5Fig.5Relativeabundancesofdifferentmethanogensontheinnerfaceofgranularsludge212,,.4COD,,2.,COD,,110~210mm,11562g/(g#d),COD7283:UASB88164%,210mm..,,.,110~210mm11231118,,.,.2CODTable2CODremovalratioandmaximumspecificmethanogenicactivityofsludgesampleswithdifferentdiameters/mm016016~110110~210210/g#(g#d)-111311113941156211467COD/%761318112188164851813(1)(),.(2)():(25150?8163)%~(48167?8187)%;110~210mm;;..(3)11311~11562g/(g#d),COD,,.:[1]ChangYJ,NishioN,NagaiS.CharacteristicsofgranularmethanogenicsludgegrownonphenolsyntheticmediumandmethanogenicfermentationofphenolicwastewaterinaUASBReactor[J].JFermentBioeng,1995,79(4):348-353.[2]FukuzakiS,NishioN,NagaiS.Highrateperformanceandcharacterizationofgranularmethanogenicsludgesinupflowanaerobicsludgeblanketreactorsfedwithvariousdefinedsubstrates[J].JFermentBioeng,1995,79(4):3