安达生态塘系统细菌的季节性变化规律

40　220082　　　　　　　　　JOURNALOFHARBININSTITUTEOFTECHNOLOGY　Vol.40No.2Feb.2008　　　　　　杨长福1,2,王爱杰2,张　颖1,王宝贞2(1.,150030;2.,150090,E-mail:[e-mail])　:为了对安达生态塘污水处理系统中细菌的季节性变化进行定性和定量分析,结合生态塘中水质条件和运行参数,分析安达生态塘系统中细菌随季节和工艺条件变化的波动性和分布规律.结果表明,季节性温度变化对厌氧塘入口处和兼性塘出口处细菌总数的影响不大,对兼性塘入口处细菌总数影响较大,同一季节生态塘中各处理单元细菌总数呈下降趋势;对3个时期种属组成相似性分析表明兼性塘中的细菌组成正随着水质条件和气候条件而动态变化.:生态塘;细菌数量;细菌种群组成;相似性分析:U416:A:0367-6234(2008)02-0333-04SeasonalvariationofbacteriainAndaeco-pondsystemYANGChang-fu1,2,WANGAi-jie2,ZHANGYing1,WANGBao-zhen2(1.SchoolofResourceandEnvironment,NortheastAgricultureUniversity,Harbin150030,China;2.SchoolofMunicipalandEnvironmentalEngineering,HarbinInstituteofTechnology,Harbin150090,China,E-mail:[e-mail])Abstract:Inordertoinvestigatetherelationshipbetweenthevariationinbacterialcommunitystructuresandtheoperatingconditionsofeco-pondprocess,functionalbacteriadistributionandshiftduringdifferentseasonsinAndaeco-pondweredetectedqualitativelyandquantitatively.TheresultsshowthatinAndaeco-pond,theamountofbacteriaintheinletofanaerobicpondandintheoutletofanoxicpondhaslittleseasonalvariation,butgreatseasonalvariationoccurrsintheinletofanoxicpond.Theamountofbacteriainoneseasondecreasesgraduallyfromtheinletofanaerobicpondtotheoutletofanoxicpond.Andtheanalysisforsimilaritycoeffi-cient(Cs)ofbacterialcommunityinthedifferentseasonsindicatesthatwaterqualityandseasonshiftgreatlyaffectthebacterialcommunitystructureandtheirtransformation.Keywords:eco-pond;theamountofbacteria;communitystructureofbacteria;similaritycoefficient:2006-04-10.:(2005800612).:(1982—),,;(1972—),,,;(1932—),,,.　　[1],-,,1.-,-()、(、)(、)[2].,[3～6],,,[7～9].,[10].,.(),,,.1　1　材料与方法1.1　20041220051、200589、200510,3;、、3.,.(),12h.1.2　0.45μm,(GB18918-2002).pH、DOSenTix41WTWMultiLineP3pH/Oximeter.1.3　,()、()[11,12].2　试验结果2.1　2.1.1　,COD76.5～124.5mg/L,99.76mg/L,[13].,,COD35%,98mg/L,72.06mg/L.,30.8mg/L,17.46mg/L,NO2-NNO3-N,1～1.5mg/L;23.1mg/L,18.48mg/L,NO2-NNO3-N1.1mg/L4.7mg/L,1.58mg/L0.7mg/L.,100%《》(GB18918-2002),,.2.1.2　pH、DO、2:pH,7.38、7.62、7.66,pH.DO,1.94mg/L、6.68mg/L、7.62mg/L;,2mg/L8mg/L,,.,8910、121,()、,.2　DO、pH2.2　2.2.1　3,,106/mL105/mL;,105/mL107/mL,,200510,4.9×107/mL.,;(910),106/mL.2.2.2　,,·334·　　　　　　　　　　　　　　　　　　　　　40　,[14,15].,,.1,38、78,3(Brevibacterium)、(Alicaligenes)(Pseudomonas)3.3　1　2005-08(Acinetobacter)、(Brevibacterium)、(Bacillus)、(Alicaligenes)、(Microbacterium)、(Pseudomonas)、(Zoogloea)、(Escherichia)2005-09(Microbacterium)、(Brevibacterium)、(Pseudomonas)、(Alicaligenes)、(Bacillus)、(Acinetobacter)、(Zoogloea)2005-10(Flavobacterium)、(Alcaligenes)、(Pseudomonas)、(Bacillus)、、(Sporosarcina)、(Agrobacterium)、(Plesiomonas)　　,Sorenson(Cs).:Cs=2j(a+b)×100.:a、b2,j2[16].2.2　Cs　%Cs2005-082005-092005-102005-0810093.3337.52005-0993.33100402005-1037.50401003　讨　论,:,COD76.5～124.5mg/L,;,(3.5m),(28.04℃),,,24.8℃32.7℃,24℃,.,,,2,.,,10℃,2.,200510.COD,,pH6.4,,..,.3,,.,.389,10,8、9103,89pH、DO,10,,38910.,910810·335·2,:,..4　结　论　　1),《》(GB18918-2002),.2),,.3)20058、91038、78;3.4),,,,.参考文献:[1],.[J].,2004,23(12):20-22.[2],,.[J].,2000,13(2):74-76.[3],,.[J].,2005,25(1):88-91.[4],,,./[J].,2004,24(6):712-716.[5]CONNOLLYR,ZHAOY,SUNG,etal.Astudyonthetreatmentofleachatesfromdifferentlandfillsitesinalab-scaleverticalflowwetlandsystememployingefflu-entrecirculation[C]//6thInternationalConferenceonWasteStabilisationPondsand9thInternationalConfer-enceonWetlandSystemsforWaterPollutionControl.A-vignon:IWA,2004.[6]LANGERGRABERG,SLEYTRK,HABERLR,etal.Experiencesonusingmineralwoolfiltersasadrainagelayerforsubsurfaceverticalflowconstructedwetlands[C]//6thInternationalConferenceonWasteStabilisa-tionPondsand9thInternationalConferenceonWetlandSystemsforWaterPollutionControl.Avignon:IWA,2004.[7],.[J].,1999,19(6):672-676.[8],,.[J].,2000,20(2):283-287.[9]PENAMR,SANABRIAIJ,MARADD.Studyonthedynamicsofthemainbacterialandarchaeagroupsinthreepilotscaleanaerobicpondsfordomesticwastewatertreatment[C]//6thInternationalConferenceonWasteStabilisationPondsand9thInternationalConferenceonWetlandSystemsforWaterPollutionControl.Avignon:IWA,2004.[10].[M].:,2002.[11].[M].:,2002.[12].[M].:,1988.[13].[M].:,2005.[14].[D].:,1988.[15].[M].:,1990.[16].DGGE[J].,2005,25(Suppl.):98-101.(　刘　彤)·336·　　　　　　　　　　　　　　　　　　　　　40