氨氮测定方法的对比研究陈一辉1

　　　201129氨氮测定方法的对比研究＊陈一辉1　李伟民1　伍　培2　刘火安3　张　勤1(1.　,400045;2.　,401331;3.　,401331)　:用酶法和纳氏试剂分光光度法对跌水曝池生物滤池的进、出水中的氨氮进行了测定,用数理统计中的t检验法对两种方法的测定值进行了比较,结果表明:酶法具有测定结果准确、测定成分浓度范围宽、干扰少等优点,此快速、简便的方法适用于水中氨氮的测定。:氨氮;酶法测定;纳氏试剂分光光度法;t验检COMPARISONOFDETERMINATIONMETHODFORAMMONIA-NITROGENChenYihui1　LiWeimin1　WuPei2　LiuHuoan3　ZhangQin1(1.SchoolofUrbanConstructionandEnvironmentalEngineering,ChongqingUniversity,Chongqing400045,China;2.SchoolofArchitectureandCivilEngineering,ChongqingCollegeofTechnology,Chongqing401331,China;3.SchoolofChemistryandChemicalEngineering,ChongqingCollegeofTechnology,Chongqing401331,China)Abstract:Ammonia-nitrogenininfluentandeffluentofbiologicalfallingwateraeratedfilterweredeterminedbyenzymaticdeterminationandN-reagentspectrophotometrymethod.Thecomparisonsbetweenthetwore-sultsweremadebymeansofttestinmathematicalstatistics,whichindicatedthattheenzymaticdeterminationhadadvantagesduetoitsaccuracy,simplicity,rapidity,widerangeofmeasurementandlessinterference,whichwassuitablefordeterminingtheammonia-nitrogeninwastewater.Keywords:ammonia-nitrogen;enzymaticdetermination;N-regentspectrophotometrymethod;ttest＊(2008ZX07315-005);(200877)。　　,、。,。,“”。、、、、—、、、、、、、—、、[1-3],,,,,、、、、,,[4]。,、,、、,,[5-6]。,、,。1　1.1　仪器与试剂UV-9100(),pHS-2D(),(),WH-1()。:105℃0.125mol/L,;[GLDH]:0.2g/L;[NADH]:0.05mol/L;a-:0.5mol/L;[Tris]:0.05234DOI:10.13205/j.hjgc.2011.s1.096　　　201129mol/L;[EDTA]:0.2mol/L;,。1.2　测定原理1.2.1　,400～500nm,。1.2.2　:NH+4+α-+NADHGLDH+NAD+H2O(NADH),NH4Cl,。1.3　样品的测定1.3.1　,50mL,50mL50mL,,,1.0mL,,1.5mL,,10min,420nm,20nm,,。1.3.2　0.05mol/LTris-HCl3mL,0.125mol/L20μL,10μL0.5mol/Lα-10μL0.05mol/LNADH,10μL0.2g/LGLDH,343nm(■A)(15s,10),。V(μmol/min)=ΔA×(mL)Δt(min)×ξ(L/mmol·cm)×b(cm):1/V=Km/(Vmax[NH4+])+1/Vmax,NH4+。2　:Y=5.2551X+0.004,R2=0.9999;1/V=0.7158/[NH4+]+0.1104,r=0.997。。2.1　不同方法的测定法果对比分析,B、(1、2、3、4、5),,t[7]。1。(2)t。1　123451234565.663.961.959.356.150.365.063.762.159.156.050.064.561.458.254.550.345.164.661.458.054.250.345.068.765.561.957.753.449.369.166.161.857.253.649.359.356.152.448.243.538.260.356.252.448.043.238.054.550.847.242.437.733.054.051.047.042.438.033.067.162.456.650.845.138.767.562.456.651.045.538.578.270.360.851.443.035.178.469.960.551.443.235.372.966.659.351.344.037.273.267.060.051.644.037.082.473.464.055.647.739.883.073.564.255.347.840.079.270.862.453.545.137.279.070.662.553.245.137.12　∑65.664.568.759.354.567.178.272.982.479.265.064.669.160.354.067.578.473.283.079.0X0.6-0.1-0.4-1.00.5-0.4-0.2-0.3-0.60.2-0.17X20.360.010.161.000.250.160.040.090.360.042.47　　X=1n∑ni=1xi=110∑10i=1xi=0.017,235　　　201129S=∑xi2-(∑xi)2nn-1=2.47-(-0.017)2109=0.524,t=X-0Sn=0.0170.52410=0.103。t,t0.05(10)=2.228,t=0.1032.228,,。3　1)t,,。2)0.025～2mg/L,0.31mg/L,。3),HgCl2,。HgCl2,,,。,,。,,,,。,,,,。[1]SMeseguerLloret,JVerdúAndrés,CMolinsLegua,eta1.Deter-minationofammoniaandprimaryaminecompoundsandKjeldahlni-trogeninwatersampleswithamodifiedRoth'sfluorimetricmethod[J].Talanta,2005(65):869-875.[2]AAminot,DSKirkwood,RKérouel.Determinationofammoniainseawaterbytheindophenol-bluemethod:EvaluationoftheICESNUTSI/C5questionnaire[J].MarineChemistry,1997(56):59-75.[3].[M].4.:,2002.[4],,.[J].,2010,32(5):27-33.[5]BagirovaNA,ShekhovtsovaTN,HuysteeRB.Enzymaticdeter-minationofphenolsusingpeanutperoxidase[J].Talanta,2001(55):1151-1164.[6]AntunesF,MarinhoHS,PihtoRE,etal.Diagnosisofenzymeinhi-tionbasedonthedegreeofinhibition[J].BiochimicaetBiophysicaAeta.2003,16(24):11-20.[7],.[M].:,1988.　　401331　503E-mail　[e-mail]2011-07-04(233),。[1].[M].:,2002:125.[2],,.[J].,1994,13(6):21-23.[3],.[J].,1992(4):7-10.[4].[M].:,1990.[5],R.D.Davls.[J].,1998,24(9):25-29.[6],,.[J].,2003(3):98-99.[7],,,.[J].,2003,23(11):2464-2474.[8],,,.[J].,2006,15(5):974-978.[9],,,.[J].,2001,20(4):273-276.[10],,,.[J].,2002,23(5):52-56.[11],,,.[J].,2003,23(5):561-569.[12],,.[J].,1994,25(3):126-129.[13],,,.[J].,1997,10(3):46-49.[14],.[J].,2009,28(2):64-69.[15],.[J].,2003(3):116-119.[16].[J].,1994,13(5):204-209.[17].[J].,1992(5):32-37.[18].[D].:,2006.[19].[D].:,2007.　　410007　34★1101E-mail　[e-mail]2011-03-28236